Cytokinin influence on in vitro shoot induction and genetic stability assessment of Dendrocalamus latiflorus Munro: a commercially important bamboo in Manipur, North-East India

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00392-5
First Page: 1085
Last Page: 1095
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Keywords: DNA markers, Somaclonal variation, Monomorphism, Cytokinins, Genetic stability


In vitro propagation of Dendrocalamus latiflorus is another alternative method to natural propagation which is limited by poor seed production and irregular and prolonged flowering cycles. The effect of BAP (6-benzylaminopurine) and KN (kinetin) was tested for bud break, shoot initiation, and multiplication during in vitro stage. Successful bud break and higher shoot formation were observed at the elevated level of KN (8.0 mg/L) or BAP (8.0 mg/L). Multiple marker systems were employed to determine genetic variation that could appear at the shooting stage due to high cytokinin concentration. A high level of monomorphism was detected among the in vitro clones with inter simple sequence repeats (ISSR) and start codon targeted polymorphism (SCoT) markers detecting 98.4% and 98% monomorphism, respectively. The inter-primer binding site (iPBS) and CAAT box-derived polymorphism (CBDP) markers also recorded 100% monomorphism. Nei’s genetic distance from the pooled ISSR, SCoT, CBDP and iPBS data showed close genetic distance values ranging from 0.000 to 0.006, disclosing high genetic identity among the genotypes. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) based dendrogram generated from combined marker data revealed a closed genetic association of the mother plant (MP) and in vitro clones (R1-R7). Principal coordinate analysis (PCoA) further confirmed the grouping of the genotypes as per the dendrogram cluster. The high monomorphism (99.1%) and close clustering patterns between the genotypes indicated the maintenance of high genetic stability at the shooting stage despite elevated levels of BAP and KN in the medium.

DNA markers, Somaclonal variation, Monomorphism, Cytokinins, Genetic stability

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The authors would like to thank the SERB, Department of Science and Technology (DST), New Delhi, India, for providing financial assistance for the research work.

Author Information

Tikendra Leimapokpam
Department of Biotechnology, Manipur University, Canchipur, India

Dey Abhijit
Department of Life Sciences, Presidency University, Kolkata, India

Jamir Imlitoshi
Department of Biotechnology, Nagaland University, Dimapur, India

Sahoo Manas Ranjan
ICAR-Indian Institute of Horticultural Research (ICAR-IIHR), Bhubaneswar, India

Nongdam Potshangbam
Department of Biotechnology, Manipur University, Canchipur, India