Vegetos
(An International Journal of Plant Research & Biotechnology)
Detection of antimicrobial resistant bacterial pathogens in the raw chicken meat samples in North India
*Article not assigned to an issue yet
Kumar Sunil, Rani Anju, Yadav Mukesh, Sehrawat Nirmala, Singh Manoj, Sharma Anil
Research Articles | Published: 31 December, 2024
First Page: 0
Last Page: 0
Views: 1815
Keywords: Chicken meat, Food-borne illness, MALDI-TOF MS, Pathogenic bacteria
Abstract
Meat and meat products offer a favorable environment for the growth and multiplication of various microorganisms because of the high nutrient content. The aim of the present study was to detect the level of microbiological contamination of retail chicken meat samples in the Mullana (Ambala) territory of Haryana, India. A total of fifty raw chicken meat samples were randomly collected from various retail butcher shops (n = 10) in the Mullana area of Ambala district. All the isolates were confirmed by MALDI-TOF MS and screened for antibiotic susceptibility testing by Kirby Bauer disk diffusion method for a set of antimicrobials. The results showed a total of eight genera of contaminating bacteria and a total of 38 bacterial isolates were retrieved from the collected 50 meat samples. The total aerobic bacterial count range was between 2.93 × 103 CFU/gm and 3.48 × 106 CFU/gm. Nearly 44% of chicken meat samples were contaminated with P. mirabilis. The majority of isolates were resistant to amoxicillin–clavulanic acid and trimethoprim–sulfamethoxazole (73%, 16/22). This study further substantiates the potential of new bacterial species found in contaminated chicken meat samples as a source of food poisoning, which is a global public health concern.
References
Abdallah HM, Al Naiemi N, Elsohaby I, Mahmoud AFA, Salem GA, Vandenbroucke-Grauls C (2022) Prevalence of extended-spectrum beta-lactamase-producing enterobacterales in retail sheep meat from Zagazig city. Egypt BMC Vet Res 18:191. https://doi.org/10.1186/s12917-022-03294-5
Askari N, Momtaz H, Tajbakhsh E (2019) Acinetobacter baumannii in sheep, goat, and camel raw meat: virulence and antibiotic resistance pattern. AIMS Microbiol 5:272–284. https://doi.org/10.3934/microbiol.2019.3.272
Bakkar F, Zubair M (2019) Molecular study based on Proteus mirabilis contamination in chicken meat in selected districts of Kerala, India. Int J Dev Res 9:28367–28369
Bantawa K, Rai K, Subba Limbu D, Khanal H (2018) Food-borne bacterial pathogens in marketed raw meat of Dharan, eastern Nepal. BMC Res Notes 11:618. https://doi.org/10.1186/s13104-018-3722-x
Bhardwaj DK et al (2021) Phenotypic and genotypic characterization of biofilm forming, antimicrobial resistant, pathogenic Escherichia coli isolated from Indian dairy and meat products. Int J Food Microbiol 336:108899. https://doi.org/10.1016/j.ijfoodmicro.2020.108899
Bhutia MO, Thapa N, Tamang JP (2020) Molecular characterization of Bacteria, detection of enterotoxin genes, and screening of Antibiotic susceptibility patterns in traditionally processed Meat products of Sikkim. India Front Microbiol 11:599606. https://doi.org/10.3389/fmicb.2020.599606
Cheesbrough M (2006) District Laboratory Practice in Tropical Countries, 2 edn. Cambridge University Press, Cambridge, UK
Chen L, Sun L, Zhang R, Liao N, Qi X, Chen J (2022) Surveillance for foodborne disease outbreaks in Zhejiang Province, China, 2015–2020. BMC Public Health 22:135. https://doi.org/10.1186/s12889-022-12568-4
CLSI (2017) Performance standards for antimicrobial susceptibility testing; 27th informational supplement. Clinical and Laboratory Standards Institute M100-27, Wayne, USA
Durgadevi R, Abirami G, Alexpandi R, Nandhini K, Kumar P, Prakash S, Veera Ravi A (2019) Explication of the potential of 2-Hydroxy-4-Methoxybenzaldehyde in hampering Uropathogenic Proteus mirabilis Crystalline Biofilm and. Virulence Front Microbiol 10:2804. https://doi.org/10.3389/fmicb.2019.02804
Eshamah HL, Naas HT, Garbaj AM, Azwai SM, Gammoudi FT, Barbieri I, Eldaghayes IM (2020) Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market. Open Vet J 10:276–288. https://doi.org/10.4314/ovj.v10i3.6
Gautam V, Sharma M, Singhal L, Kumar S, Kaur P, Tiwari R, Ray P (2017) MALDI-TOF mass spectrometry: an emerging tool for unequivocal identification of non-fermenting gram-negative bacilli. Indian J Med Res 145:665–672. https://doi.org/10.4103/ijmr.IJMR_1105_15
Guo S, Aung KT, Tay MYF, Seow KLG, Ng LC, Schlundt J (2019) Extended-spectrum beta-lactamase-producing Proteus mirabilis with multidrug resistance isolated from raw chicken in Singapore: genotypic and phenotypic analysis. J Glob Antimicrob Resist 19:252–254. https://doi.org/10.1016/j.jgar.2019.10.013
Hassan M, Vittal R, Raj JM, Chakraborty G (2022) Loop-mediated isothermal amplification (LAMP): a sensitive molecular tool for detection of Staphylococcus aureus in meat and dairy product. Braz J Microbiol 53:341–347. https://doi.org/10.1007/s42770-021-00659-0
Jain S, Gaind R, Kothari C, Sehgal R, Shamweel A, Thukral SS, Chellani HK (2016) VEB-1 extended-spectrum beta-lactamase-producing multidrug-resistant Proteus mirabilis sepsis outbreak in a neonatal intensive care unit in India: clinical and diagnostic implications. JMM Case Rep 3:e005056. https://doi.org/10.1099/jmmcr.0.005056
Jiang X, Yu T, Liu L, Li Y, Zhang K, Wang H, Shi L (2017) Examination of Quaternary Ammonium Compound Resistance in Proteus mirabilis isolated from Cooked Meat products in. China Front Microbiol 8:2417. https://doi.org/10.3389/fmicb.2017.02417
Khater DF, Lela RA, El-Diasty M, Moustafa SA, Wareth G (2021) Detection of harmful foodborne pathogens in food samples at the points of sale by MALDT-TOF MS in Egypt. BMC Res Notes 14:112. https://doi.org/10.1186/s13104-021-05533-8
Klaharn K, Pichpol D, Meeyam T, Harintharanon T, Lohaanukul P, Punyapornwithaya V (2022) Bacterial contamination of chicken meat in slaughterhouses and the associated risk factors: a nationwide study in Thailand. PLoS ONE 17:e0269416. https://doi.org/10.1371/journal.pone.0269416
Kumar S, Anwer R, Sehrawat A, Sehrawat N, Yadav M, Sharma A (2022a) Isolation and characterization of pathogenic bacteria from drinking water in North India. Int J Environ Sci Technol 19:12605–12610. https://doi.org/10.1007/s13762-021-03774-5
Kumar S, Anwer R, Sehrawat A, Yadav M, Sehrawat N (2021a) Assessment of bacterial pathogens in drinking water: a serious safety concern. Curr Pharmacol Rep 7:206–212. https://doi.org/10.1007/s40495-021-00263-8
Kumar S, Anwer R, Yadav M, Sehrawat N, Kumar V, Sharma A K (2021b) Isolation and Characterization of Acinetobacter baumannii from Chicken Meat Samples in North India. Asian J Biol Life Sci 10:462–468 https://doi.org/10.5530/ajbls.2021.10.61
Kumar S, Saifi Z, Sharma A, Upadhyay S (2020) Rapid Identification of Clinical isolates of Klebsiella pneumoniae using MALDI-TOF MS from North India. Bull Pure Appl Sci (Zoology) 39:194–199. https://doi.org/10.5958/2320-3188.2020.00022.4
Kumar S, Yadav M, Devi A, Uniyal M, Kumar V, Sehrawat N, Singh R (2022b) Assessment of pathogenic micro-organisms Associated with Vegetable salads. Asian J Biol Life Sci 11:1–7. https://doi.org/10.5530/ajbls.2022.11.1
Ma Y, Su XZ, Lu F (2020) The roles of type I Interferon in Co-infections with parasites and viruses, Bacteria, or other. Parasites Front Immunol 11:1805. https://doi.org/10.3389/fimmu.2020.01805
Mathew B, Nanu E, Sunil B (2014) Assessment of bacterial quality of market beef. Int J Basic Life Sci 2:1–7
Milton AAP et al (2021) Development of novel visual detection methodology for Salmonella in meat using saltatory rolling circle amplification. J Appl Microbiol 131:2361–2371. https://doi.org/10.1111/jam.15099
Peruzy MF et al (2022) Presence of enteric bacterial pathogens in meat samples of wild boar hunted in Campania region, southern Italy Ital. J Food Saf 11:9967. https://doi.org/10.4081/ijfs.2022.9967
Sanches MS et al (2021) Proteus mirabilis from community-acquired urinary tract infections (UTI-CA) shares genetic similarity and virulence factors with isolates from chicken. beef pork meat Microb Pathog 158:105098. https://doi.org/10.1016/j.micpath.2021.105098
Srey S, Jahid I, Ha S (2013) Biofilm formation in food industries: a food safety concern. Food Control 31:572–585. https://doi.org/10.1016/j.foodcont.2012.12.001
Stanborough T, Fegan N, Powell SM, Singh T, Tamplin M, Chandry PS (2018) Genomic and metabolic characterization of spoilage-associated Pseudomonas species. Int J Food Microbiol 268:61–72. https://doi.org/10.1016/j.ijfoodmicro.2018.01.005
Tieland M, Borgonjen-Van den Berg KJ, van Loon LJ, de Groot LC (2012) Dietary protein intake in community-dwelling, frail, and institutionalized elderly people: scope for improvement. Eur J Nutr 51:173–179. https://doi.org/10.1007/s00394-011-0203-6
Vangchhia B, Blyton MDJ, Collignon P, Kennedy K, Gordon DM (2018) Factors affecting the presence, genetic diversity and antimicrobial sensitivity of Escherichia coli in poultry meat samples collected from Canberra. Australia Environ Microbiol 20:1350–1361. https://doi.org/10.1111/1462-2920.14030
Wardhana DK, Haskito AEP, Purnama MTE, Safitri DA, Annisa S (2021) Detection of microbial contamination in chicken meat from local markets in Surabaya, East Java. Indonesia Vet World 14:3138–3143. https://doi.org/10.14202/vetworld.2021.3138-3143
WHO (2020) Clinical consortium on healthy aging 2019: report of consortium meeting held 21–22 November 2019. World Health Organization, Geneva, Switzerland
WHO (2021) Global launch of FAO/WHO Food Control System Assessment Tool. World Health Organization, Geneva, Switzerland
Yang L et al (2021) Nosocomial outbreak of carbapenemase-producing Proteus mirabilis with two novel Salmonella genomic island 1 variants carrying different bla (NDM-1) gene copies in China. Front Microbiol 12(800938). https://doi.org/10.3389/fmicb.2021.800938
Yang R, Xu G, Wang X, Qing Z, Fu L (2020) Establishment and application of a dual TaqMan Real-Time PCR method for Proteus Mirabilis and Proteus Vulgaris. Pol J Microbiol 69:293–300. https://doi.org/10.33073/pjm-2020-032
Yari AR, Mohammadi MJ, Geravandi S, Doosti Z, Matboo SA, Jang SA, Nazari S (2018) Assessment of microbial quality of household water output from desalination systems by the heterotrophic plate count method. J Water Health 16:930–937. https://doi.org/10.2166/wh.2018.082
Zhang Y, Zhang J, Chang X, Qin S, Song Y, Tian J, Ma A (2022) Analysis of 90 Listeria monocytogenes contaminated in poultry and livestock meat through whole-genome sequencing. Food Res Int 159:111641. https://doi.org/10.1016/j.foodres.2022.111641
Zheng Y et al (2021) RPA-SYBR green I based instrument-free visual detection for pathogenic Yersinia enterocolitica. meat Anal Biochem 621:114157. https://doi.org/10.1016/j.ab.2021.114157
Author Information
Department of Microbiology, Graphic Era (Deemed to be University), Dehradun, India