Determination of antioxidant and antibacterial properties of Tradescantia spathacea root extracts

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00558-1
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Keywords: Commelinaceae, n Tradescantia spathacean , Phytochemical screening, DPPH, FRAP


Tradescantia spathacea Sw. (T. spathacea) is known for its antioxidant properties as it was used as a traditional medicine from the 16th to 19th century, and little research has been done on its phytochemical analysis on the roots. In this study, hexane and ethyl acetate root extracts of T. spathacea were analyzed for phytochemical, antioxidant and antibacterial properties, such as total phenolic content (TPC), total flavonoid content (TFC), free radical scavenging (DPPH), ferric reducing antioxidant power (FRAP), disc diffusion (DF) and broth microdilution (MD) assay. The highest concentration of TPC and TFC calculated from the ethyl acetate extract was 243 ± 11 mg GAE/g DW and 101 ± 4 mg CE/g DW, respectively. The ethyl acetate extract screened for the DPPH assay showed the highest EC50 of 88 ± 16 µg/mL. Similarly, a FRAP assay was conducted giving the highest value of 6.13 ± 0.87 mmol Fe2+/g DW from the ethyl acetate extract of T. spathacea roots. Both extracts were screened for antibacterial activity against four species of bacterium. The root extracts of T. spathacea contain a sizeable amount of antioxidant properties, proposing a potential use of this plant as a source of natural antioxidants.

Commelinaceae, n              Tradescantia spathacean            , Phytochemical screening, DPPH, FRAP

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This study was supported by Tunku Abdul Rahman University College Publication Incentive (Votehead No. 86000-4923).

Author Information

Lim Bryan Chi Wah
Department of Physical Science, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia

Keng Xin-Yi
Department of Physical Science, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia

Loh Khye-Er
Department of Bioscience, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia

Tan Siow-Ping
Department of Physical Science, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia