Determination of antioxidant and antibacterial properties of Tradescantia spathacea root extracts

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00558-1
First Page: 1475
Last Page: 1482
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Keywords: Commelinaceae, n Tradescantia spathacean , Phytochemical screening, DPPH, FRAP


Abstract


Tradescantia spathacea Sw. (T. spathacea) is known for its antioxidant properties as it was used as a traditional medicine from the 16th to 19th century, and little research has been done on its phytochemical analysis on the roots. In this study, hexane and ethyl acetate root extracts of T. spathacea were analyzed for phytochemical, antioxidant and antibacterial properties, such as total phenolic content (TPC), total flavonoid content (TFC), free radical scavenging (DPPH), ferric reducing antioxidant power (FRAP), disc diffusion (DF) and broth microdilution (MD) assay. The highest concentration of TPC and TFC calculated from the ethyl acetate extract was 243 ± 11 mg GAE/g DW and 101 ± 4 mg CE/g DW, respectively. The ethyl acetate extract screened for the DPPH assay showed the highest EC50 of 88 ± 16 µg/mL. Similarly, a FRAP assay was conducted giving the highest value of 6.13 ± 0.87 mmol Fe2+/g DW from the ethyl acetate extract of T. spathacea roots. Both extracts were screened for antibacterial activity against four species of bacterium. The root extracts of T. spathacea contain a sizeable amount of antioxidant properties, proposing a potential use of this plant as a source of natural antioxidants.


Commelinaceae, n              Tradescantia spathacean            , Phytochemical screening, DPPH, FRAP


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References


Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 237:70–76


Chan YS, Khoo KS, Sit NWW (2016) Investigation of twenty selected medicinal plants from Malaysia for anti-Chikungunya virus activity. Int Microbiol 19:175–182. https://doi.org/10.2436/20.151.01.275


Cho KH, Tan SP, Tan HY et al (2022) Morphinan alkaloids from leaves of Alphonsea cylindrica and their antibacterial properties. Planta Med 88:1–7. https://doi.org/10.1055/a-1797-0548


Clinical and Laboratory Standards Institute (CLSI) (2012) Performance standards for antimicrobial disk susceptibility tests: approved standard–11th edition. Clinical and Laboratory Standards Institute, Wayne


Clinical and Laboratory Standards Institute (CLSI) (2014) Performance standards antimicrobial susceptibility testing twenty-fourth informational supplement. Clinical and Laboratory Standards Institute, Wayne


De la Rosa LA, Moreno-Escamilla JO, Rodrigo-Garcia J, Alvarez-Parrilla E (2019) Chapter 12 phenolic compounds. Postharvest physiology and biochemistry of fruits and vegetables. Elsevier, Amsterdam, pp 253–271. https://www.researchgate.net/profile/Armando-Carrillo-Lopez/publication/329414342_Postharvest_Physiology_and_Biochemistry_of_Fruits_and_Vegetables/links/5c074a4992851c6ca1ff2124/Postharvest-Physiology-and-Biochemistry-of-Fruits-and-Vegetables.pdf. Accessed 16 Dec 2022


European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2022) EUCAST reading guide for broth microdilution version 4.0


Ghafar F, Nazrin TNNT, Salleh MRM et al (2017) Total phenolic content and total flavonoid content in Moringa Oleifera seed. Sci Herit J. 1:23–25. https://doi.org/10.26480/gws.01.2017.23.35


Global Cancer Observatory (2021) Malaysia. Available via https://gco.iarc.fr/today/data/factsheets/populations/458-malaysia-fact-sheets.pdf. Accessed 16 Dec 2022


Blois MS (1958) Antioxidant determinations by the use of a stable free radical. Nature 181:1199–1200. https://doi.org/10.1038/1811199a0


Halimatussakdiah AU, Tan SP et al (2015) In vitro cytotoxic effect of indole alkaloids from the roots of Kopsia singapurensis Ridl. against the human promyelocytic leukemia (HL-60) and the human cervical cancer (HeLa) cells. Int J Pharm Sci Res 31:89–95


Haminiuk CWI, Plata-Oviedo MSV, De Mattos G et al (2014) Extraction and quantification of phenolic acids and flavonols from Eugenia pyriformis using different solvents. Food Sci Technol 51:2862–2866. https://doi.org/10.1007/s13197-012-0759-z


Klaunig JE (2020) Carcinogenesis. An introduction to interdisciplinary toxicology, 1st edn. Elsevier, Amsterdam, pp 97–110.  https://www.sciencedirect.com/science/article/pii/B9780128136027000089. Accessed 16 Dec 2022


Kyaw EH, Kato-Noguchi H (2021) Assessment of allelopathic activity of Tradescantia spathacea Sw. for weed control. Biol Futura 72:489–495. https://doi.org/10.1007/s42977-021-00098-w


Langeland KA, Burks KC (1998) Identification and biology of non-native plants in Florida’s natural areas Gainesville. University of Florida, Florida, p 165


Lobo V, Patil A, Phatak A et al (2010) Free Radicals, antioxidant and functional foods: Impact on human health. Pharmacogn Rev 4:118–126. https://doi.org/10.4103/0973-7847.70902


Nafiah MA, Tan SP, Khoo JHC et al (2016) A new aporphine alkaloid from the leaves of Alseodaphne corneri Kosterm (Leuraceae). Tetrahedron Lett 57:1537–1539. https://doi.org/10.1016/j.tetlet.2016.02.085


Noor NAM, Nafiah MA, Johari SATT et al (2019) Anticancer effect of Hypophyllanthin, Niranthin and Lintetralin from Phyllanthus amarus on HeLa cells and NIH/3T3 cells. Int J Recent Technol Eng 8:106–110


Pedersen B, Koktved DP, Nielsen LL (2013) Living with side effects from cancer treatment - a challenge to target information. Scand J Caring Sci 27:715–723. https://doi.org/10.1111/j.1471-6712.2012.01085.x


Phuyal N, Jha PK, Raturi PP, Rajbhandary S (2020) Total phenolic, flavonoid contents, and antioxidant activities of fruit, seed, and bark extracts of Zanthoxylum armatum DC. Sci World J. https://doi.org/10.1155/2020/8780704


Prajapati T, Shukla A, Modi N (2020) Tradescantia spathacea Sw.: a review of its pharmacological and ethnopharmacological properties. Int J Res Cult Soc 4:52–56


Prieto JM (2012) Dr. Prieto’s DPPH Microplate Protocol. https://www.researchgate.net/file.PostFileLoader.html?id=503cd1c9e39d5ead11000043&assetKey=AS%3A271744332435456%401441800305338. Accessed 16 Dec 2022


Rose PG, Bundy BN, Watkins EB et al (1999) Concurrent cisplatin-based radiotherapy and chemotherapy for locally advanced cervical cancer. N Engl J Med 340:1144–1153. https://doi.org/10.1056/NEJM199904153401502


Rusell AD (1999) Bacterial resistance to disinfectants: present knowledge and future problems. J Hosp Infect 43:57–58. https://doi.org/10.1016/S0195-6701(99)90066-X


Sachett A, Gallas-Lopes M, Conterato GMM et al (2021) Antioxidant activity by FRAP assay: in vitro protocol. Available via https://www.protocols.io/view/antioxidant-activity-by-frap-assay-in-vitro-protoc-btqrnmv6. Accessed 16 Dec 2022


Santos Sánchez NF, Salas-Coronado RS, Villanueva C, Hernández-Carlos B (2019) Antioxidant compounds and their antioxidant mechanism. Antioxidants pp 1–28


Shaikh JR, Patil M (2020) Qualitative tests for preliminary phytochemical screening: an overview. Int J Chem Stud 8:603–608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834


Talip MA, Azziz SSSA, Wong CF et al (2017) New azafluorenone derivative and antibacterial activities of Alphonsea cylindrica barks. Nat Prod Sci 23(3):151–156


Tan JBL, Lim YY, Lee SM (2010) Antioxidant and antibacterial activity of Rhoeo spathacea (Swartz) Stearn leaves. J Food Sci Technol 52:2394–2400. https://doi.org/10.1007/s13197-013-1236-z


Tan SP, Lim SM, Wong MK et al (2020) Chemical constituents of Murraya koenigii berries. Chem Nat Compd 56:962–963. https://doi.org/10.3390/antiox9020101


Tan SP, Tan ENY, Lim QY et al (2021) Steroids and terpenoids from the bark of Phyllanthus acidus. Chem Nat Compd 57:385–387


Vo QH, Nguyen PH, Zhao BT et al (2015) Protein tyrosine phosphatase 1B (PTP1B) inhibitory constituents from the aerial parts of Tradescantia spathacea Sw. Fitoterapia 103:113–121. https://doi.org/10.1016/j.fitote.2015.03.017


WHO (2021) Noncommunicable diseases. https://www.who.int/health-topics/noncommunicable-diseases#tab=tab_1. Accessed 16 Dec 2022


WHO (2022) The annual health-care cost of cardiovascular diseases, diabetes and cancer in Malaysia exceeds RM 9.65 billion. https://www.who.int/malaysia/news/detail/09-08-2022-the-annual-health-care-cost-of-cardiovascular-diseases--diabetes-and-cancer-in-malaysia-exceeds-rm-9.65-billion. Accessed 16 Dec 2022


Wiegand I, Hilpert K, Hancock REW (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3(2):163–175. https://doi.org/10.1038/nprot.2007.521


Yan Y, Li X, Zhang C et al (2021) Research Progress on antibacterial activities and mechanisms of natural alkaloids: a review. Antibiotics 10:318. https://doi.org/10.3390/antibiotics10030318

 


Acknowledgements


This study was supported by Tunku Abdul Rahman University College Publication Incentive (Votehead No. 86000-4923).


Author Information


Lim Bryan Chi Wah
Department of Physical Science, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia

Keng Xin-Yi
Department of Physical Science, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia


Loh Khye-Er
Department of Bioscience, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia


Tan Siow-Ping
Department of Physical Science, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Kuala Lumpur, Malaysia

tansp@tarc.edu.my