Differential in vitro morphology exhibited by pigeonpea seedlings in response to plant growth regulators

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00352-z
First Page: 656
Last Page: 662
Views: 1772

Keywords: Pigeonpea, Apical dominance, In vitro, Growth regulators, Morphology


The effect of benzyl aminopurine (BAP), thidiazuron (TDZ), and indole butyric acid (IBA) on seedling morphology in two pigeonpea varieties, viz ‘Vipula’ and ‘Phule Rajeshwari’ were studied. Primary shoot growth suppression occurs at higher growth regulator concentration, leading to the outgrowth of axillary buds. Prevention of axillary bud initiation in auxin supplemented medium emphasizes the role of auxin in apical dominance. The adventitious shoot buds, bordered by a spiral of meristematic tissue, were formed at the pre-existing axillary bud in a cytokinin supplemented medium. The highest multiple shoot formation (5.7 per explants) was observed on 0.2 mg/l BAP supplemented MS medium. Plant growth regulators induced many shoot and root morphology variations in both cultivars. The numbers of roots per seedling varied from 13.2 to 1. A higher shoot abnormality was recorded in seedlings grown in TDZ supplemented medium. The current study highlighted how plant growth regulators influence the morphological response of in vitro regenerated plants.

Pigeonpea, Apical dominance, In vitro, Growth regulators, Morphology

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Author Information

Pawar Bhausaheb
State Level Biotechnology Centre, Mahatma Phule Agricultural University, Rahuri, India