DNA barcoding and antioxidant study of four Dendrobium species found in Manipur

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00517-w
First Page: 1313
Last Page: 1325
Views: 1388


Keywords: n Dendrobiumn , DNA barcoding, n matK gene, Antioxidant activity


Abstract


Dendrobium species are epiphytic orchids known for their aesthetic beauty however they are also reported to manifest a diversity of medicinal effects. It has been assigned to a large number of pharmacological activities. Proper use of medicinal herbs increases the efficacy of the herbal drug. The present article studies the DNA Barcoding of four Dendrobium species and their antioxidant properties. In this study, we have used the matK gene as a marker for DNA barcoding to differentiate the species of Dendrobium. The amplified PCR product was purified and sequenced. Analysis of the obtained sequencing reads using computational tools validated the presence of the matK gene in all four Dendrobium species. Results of the sequence homology search studies using the BLAST tool showed a high degree of percent similarity of sequenced matK gene against reported matK gene sequences. Also, the matK gene sequence of four Dendrobium species exhibited  a high degree of conservation as revealed by multiple sequence alignment. The antioxidant studies of polysaccharides obtained from Dendrobium showed that all the species exhibit antioxidant properties with the most significant IC50 value of 69.41 µg/ml in DPPH assay and 222.6 µg/ml in NO radical scavenging assay of D. palpebrae.


n              Dendrobiumn            , DNA barcoding, n                     matK gene, Antioxidant activity


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Acknowledgements


The authors are grateful to the Department of Life Sciences (Botany), Manipur University; Ramjas College, University of Delhi, and SGTB Khalsa College, University of Delhi for providing the necessary requirements for conducting the experiments.


Author Information


Sunanda Yumnam
Department of Life Sciences (Botany), Plant Physiology Section, Manipur University, Canchipur, India

Bhardwaj Ankur R.
Department of Botany, Ramjas College, University of Delhi, New Delhi, India


Mikawlrawng Khaling
Department of Life Sciences (Botany), Plant Physiology Section, Manipur University, Canchipur, India


Pandey Ritu
Department of Botany, SGTB Khalsa College, University of Delhi, New Delhi, India


Sarangthem Kananbala
Department of Life Sciences (Botany), Plant Physiology Section, Manipur University, Canchipur, India
kananbala_s@rediffmail.com