DPPH and FRAP assays for different extracts of in vitro and in vivo grown plantlets of Bacopa monnieri L.

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00547-4
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Keywords: Micro-propagation, Plant growth regulators, Methanol, Ethanol, Absorbance, Antioxidant, Radicals


Overproduction of reactive oxygen species can induce oxidative damage to all biomolecules which may lead to numerous health hazards including cancer and neurological disorders. Plant based antioxidant compounds are very essential for averting these degenerative reactions produced by free radicals and reactive oxygen species. If the plant based antioxidants are extracted from in vitro grown plantlets there shall be less dependency on natural flora. Keeping it in view, in the present study the antioxidant potentials of field grown (S1) and micropropagated (S2) plants was intended. It dealt with DPPH and FRAP bioassay methods for evaluating the free radical scavenging activity and reducing power of in vivo and in vitro grown plantlets of Bacopa monnieri L. DPPH, which is a stable free radical pairs with a hydrogen donor antioxidant and is reduced to DPPH-H form. In FRAP assay, ferric- tripyridyltriazine (FIII-TPTZ) is reduced to ferrous-tripyridyltriazine (FII-TPTZ) producing a blue-colored product in presence of antioxidant compound of Bacopa monnieri L.These two tests have been used to evaluate the free radical scavenging activity of the extracts of medicinal plants having antioxidant properties. In vitro grown plantlets were raised on MS media supplemented with different concentration and combination of plant growth hormones like NAA, KN, 2,4,D, adenine sulphate etc.

For evaluating the antioxidant properties of both the samples (S1 and S2), aqueous, ethanolic (30%) and methanolic (30%) extracts were screened. Highest level of antioxidant activity was observed in the methanolic extract of sample 2 of Bacopa monnieri L. which can be considered as new source of natural antioxidant.

Micro-propagation, Plant growth regulators, Methanol, Ethanol, Absorbance, Antioxidant, Radicals



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Author is thankful to the Joint Secretary UGC, Eastern Regional office, Kolkata for providing financial Support in the form of Minor Research Project. Author is also thankful to Dr. Madhupurna Banerjee for giving assistance during plant tissue culture work and Mr. Debashish Sahoo for technical support for biochemical studies.

Author Information

Mehta Anita
Department of Botany, Ranchi University, Ranchi, India