Keywords: Organic inputs, White root rot, Mycelial growth inhibition, Disease control
The present study describes the efficacy of three organic formulations namely Sanjivak, Jeevamrit and Cow Urine Decoction (CUD) against Dematophora necatrix, inciting white root rot of apple. Under laboratory studies, all the formulations were tested at five concentrations ranging from 2.5 to 20.0 per cent. Among all, CUD was found most effective and significantly superior over treatments exhibiting 75.93 per cent mycelial inhibition in growth of D. necatrix followed by Sanjivak (70.22%). However, Jeevamrit was found to be least effective with mycelial growth inhibition of 63.48 per cent. CUD at 20 per cent concentration provided complete (100%) mycelial growth inhibition of D. necatrix. Under pot conditions, CUD at 40 and 50 per cent and Sanjivak at 50 per cent concentrations were most effective and provided cent per cent control of the disease. Furthermore, in case of virgin and sick nursery conditions, maximum control of disease was recorded with CUD at 50 per cent concentration which provided 93.83 and 88.51 per cent disease control, respectively. The results obtained suggested that organic formulations used in this study have tremendous potential for controlling the root rot disease in apple and therefore could be deployed as efficient green way for management of white root rot disease in future.
Alabouvette C, Olivain C, Steinberg C (2006) Biological control of plant diseases: the European situation. Eur J Plant Pathol 114:329–341
Anonymous (1962) Melia the wealth of India: a dictionary of Indian raw materials and industrial products. LM, vol 6. CSIR, New Delhi, pp 323–325
Anonymous (2009) http://www.nhb.gov.in
Badadani MS, Babu SV, Shetty KT (2007) Optimum conditions of autoclaving for hydrolysis of proteins and urinary peptides of prolyl and hydroxyprolyl residues and HPLC analysis. J Chromatogr 847:267–274
Basak AB, Lee MW (2005) In vitro inhibitory activity of cow urine and cow dung on Fusarium solani f. sp. cucurbitae. Mycobiol 30:51–54
Danish M, Hisamuddin Robab MI (2015) In vitro studies on phytochemical screening of different leaf extracts and their antifungal activity against seed borne pathogen Aspergillus niger. J Plant Pathol Microbiol 6:320
Dorothy ET, Frisvad JC (2002) Eupenicillium bovifimosum, a new species from dry cow manure in wyoming. Mycologia 94:240–246
Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley, New York, p 680p
Hansen HN, Thomas HE (1937) The connection between Dematophora necatrix and Rosellinia necatrix. Hilgardia 10:561–565
Huang N, Wang W, Yao Y, Zhu F, Wang W, Chang X (2017) The influence of different concentrations of bio-organic fertilizer on cucumber Fusarium wilt and soil microflora alterations. PLoS One 12:1–17
Ito SI, Nakamura N (1984) An outbreak of white root rot and its environmental conditions in the experimental arboretum. J Jpn SocHortic Sci 66:262–267
Johri JK, Balasubrahmanyam VR, Misra G, Nigam SK (1994) Botanicals for management of betelvine diseases. Natl Acad Sci Lett 17:7–8
Kekuda PTR, Vivek MN, Manasa M, Kambar Y, Nawaz NAS, Raghavendra HL (2014) Antifungal effect of cow urine extracts of selected plants against Colletotrichum capsici isolated from anthracnose of chilli. Int J Agric Crop Sci 7:142–146
Kurosaki N, Yamato O, Sasamato Y, Mori F, Imoto S, Kojima T, Maede Y (2007) Clinico-pathological finding in peripartum dairy cows fed anions salts lowering the dietary cation-anion difference: Involvement of serum inorganic phosphorus, chloride and plasma estrogen concentration in milk fever. Jpn J Vet Res 55:3–12
Manikandan G (2005) Organic methods of pest and disease management. Centre for Indian Knowledge System, Chennai, pp 18
Mbong G, Nde T, Manju E, Kinge T, Foncha F, Mbong S (2021) Inhibitory activities of sole cow urine and combined cow dung/cow urine against the blight disease of ribwort (Plantago lanceolata) at the cistercian monastery in Mbengwi, Cameroon. Agric Sci 12:1307–1320
Muhammad A (2003) In vitro inhibition of growth of some seedling blight inducing pathogens by compost inhibiting microbes. Afr J Biotechnol 2:161–164
Naik MK (2003) Challenges and opportunities for the research in soil borne plant pathogens with special reference to Fusarium species. J Mycol Plant Pathol 33:1–4
Pal J (2019) Studies on management of white root rot (Dematophora necatrix Hartig) of apple in Himachal Pradesh. Ph. D. Thesis, Dr. YS Parmar University of Horticulture and Forestry, Solan (HP), India, pp 202
Parween T, Jan S, Mahmooduzzafar S, Fatma T, Siddiqui ZH (2016) Selective effect of pesticides on plant—a review. Crit Rev Food Sci Nutr 56:160–179
Pathak RK, Ram RA (2013) Bio-enhancers: a potential tool to improve soil fertility, plant health in organic production of horticultural crops. Progress Hortic 45:237–254
Patil PV (2007) Eco-friendly approaches for the management of soybean rust in Karnataka. Nation. Symp. Microbial. Diver. Pl. Health. Organized by Indian Mycological Society, Culcutta, (Kolkata) and Department of Plant Pathology, Nadia, pp 45.
Rani A, Bhat MN, Singh BP (2006) Efficacy of neem formulations against late blight of potato in subtropical plains. Crop Res 31:179–180
Shanthi S, Elamathy S, Panneerselvam A, Radha N (2011) Antixanthomonas activity of Pongamia pinnata linn leaves- cow urine extract a natural cost effective ecofriendly remedy to bacterial leaf blight of paddy (BLB). J Pharm Res 4:650–652
Sharma JN Sharma RC (2008) Soil borne diseases of temperate fruits and their management. In: Naik MK, Devika Rani GS (eds) Advances in soil borne plant diseases. New India Publishing Agency, New Delhi, pp 219–231
Sharma SK, Sharma M, Gupta M (2005) Role of bacterial antagonists in the management of white root rot in apple. Integrated plant disease management. In: Challenging problems in horticultural and forest pathology Solan, India 39–46
Sharma IM, Negi HS, Sharma S (2014) Integrated management of collar rot in apple caused by Phytophthora cactorum. Indian Phytopathol 67:168–173
Shwetha GS, Hegde YR (2012) Management of Sclerotium wilt of Stevia rebaudiana through biorationals. Int J Plant Prot 5:248–251
Singh S (2001) Cow urine has anti Leshmania donovani effect in vitro. Int J Cow Sci 1:72–73
Sreenivasa MN, Naik N, Bhat SN (2011) Nutrient status and microbial load of different organic liquid manures. Karnataka J Agric Sci 24:583–585
Upadhyay RK, Dwivedi P, Ahmad S (2010) Antimicrobial activity of photoactivated cow urine against certain pathogenic bacterial strains. Afr J Biotechnol 9:518–522
Vijaylakshmi K, Arumugasmy S, Subhashini S (2005) Organic methods of pest and disease management, Centre for Indian Knowledge Systems, pp 8.
Vincent JM (1947) Distortion of fungal hyphae in the presence of certain inhibitors. Nature 159:850–850
Department of Plant Pathology, Dr. Y. S. Parmar University of Horticulture and Forestry, Solan, India