Vegetos
(An International Journal of Plant Research & Biotechnology)
Effect of TiO2 and ZnO nanoparticles on culture establishment in micropropagation of pineapple (Ananas comosus)
Research Articles | Published: 23 April, 2025
First Page: 875
Last Page: 882
Views: 443
Keywords: Antimicrobial, Pineapple, Surface sterilization, TiO2 nanoparticles, ZnO nanoparticles
Abstract
Micropropagation of pineapple is used to produce a large number of true-to-type propagules. Mercuric chloride (HgCl2) has been commonly used in the past for eliminating in vitro contaminations in most field-grown explants by commercial growers. However, it is a toxic chemical and its sale is highly restricted. Therefore, this study attempted to find an alternative material for this purpose in place of HgCl2. Hence, the possibility of using environmentally friendly nanoparticles possessing antimicrobial activity for the surface sterilization of pineapple was explored. Therefore, we evaluated the potential effects of TiO2 (100 mgl− 1, 200 mgl− 1and 300 mgl− 1) and ZnO (300 mgl− 1, 400 mgl− 1 and 500 mgl− 1) nanoparticles on the survival, mortality, and contamination percentage of pineapple shoots at the culture establishment stage of micropropagation. The results showed that surface sterilization of explants with 100% Clorox (5.25% NaOCl) for 20 min followed by 400 mgl− 1 ZnO nanoparticles for 30 min resulted in the highest survival percentage (85.7%) with minimum contamination percentage (14.3%), compared to 100% survival and zero mortality obtained with immersion in 0.2% HgCl2 for 30 min. The 500 mgl− 1 ZnO nanoparticles resulted significant mortality. The use of TiO2 nanoparticle treatment, both at 100 mgl− 1 and 200 mgl− 1 concentration showed the highest survival percentage (83.3%) with zero mortality showing a promising potential for future applications.
References
Ahmad NS, Abdullah N, Yasin FM (2019) Antifungal activity of titanium dioxide nanoparticles against Candida albicans. BioRes 14(4):8866–8878
Kotb O, Atawia A, Abd El-Latif F, El-Gioushy S, Sherif S (2016) Studies on micropropagation of pineapple (Ananas comosus L). Middle East J Agric Res 5(2):224–232
Das MP, Rebecca LJ, Sharmila S, Chatterjee S (2012) Study on the effect of mercury (II) chloride as disinfectant on mixed culture. J Chem Pharm Res 4:4975–4978
Fernando SSN, Gunasekara T, Holton J (2018) Antimicrobial nanoparticles: applications and mechanisms of action, Sri Lankan. J Infect Dis 8(1):2–11. https://doi.org/10.4038/sljid.v8i1.8167
He L, Liu Y, Mustapha A, Lin M (2011) Antifungal activity of zinc oxide nanoparticles against Botrytis cinerea and Penicillium expansum. Microbiol Res 166(3):207–215. https://doi.org/10.1016/j.micres.2010.03.003
Helaly MN, El-Metwally MA, El-Hoseiny H, Omar SA, El-Sheery NI (2014) Effect of nanoparticles on biological contamination of’in vitro’cultures and organogenic regeneration of banana. Aust J Crop Sci 8(4):612–624
Jan A, Bhat KM, Bhat SJA, Mir MA, Bhat MA, Imtiyaz A, Rather JA (2013) Surface sterilization method for reducing microbial contamination of field grown strawberry explants intended for in vitro culture. Afr J Biotechnol, 12(39)
Khajuria AK, Negi A, Bisht NS, Mayura V, Kandwal A (2019) Green synthesis, characterization and antimicrobial activity of synthesized zinc oxide nanoparticles using root Ex.ract of viola canescens wall. Ex. Roxb. Asian J Chem 31(3):551–554. https://doi.org/10.14233/ajchem.2019.21738
Kim DH, Gopal J, Sivanesan I (2017) Nanomaterials in plant tissue culture: the disclosed and undisclosed. RSC Adv 7(58):36492–36505. https://doi.org/10.1039/C7RA07025J
Kiss E, Kiss J, Gyulai G, Heszky LE (1995) A novel method for rapid micropropagation of pineapple. HortScience 30(1):127–129. https://doi.org/10.21273/HORTSCI.30.1.127
Usman IS, Abdulmalik MM, Sani LA, Muhammad AN (2013) Development of an efficient protocol for micropropagation of pineapple (Ananas comosus L. Var. Smooth cayenne). Afr J Agric Res 8(18):2053–2056. https://doi.org/10.5897/AJAR11.1746
Maheepala GHN, de Silva D, Kaliyadasa PE, Perera GYADD (2018) Effect of growth regulators on In-vitro shooting of Calathea Ornata for commercial cultivation. J Food Agric 11(2). https://doi.org/10.4038/jfa.v11i2.5214
Mandeh M, Omidi M, Rahaie M (2012) In vitro influences of TiO2 nanoparticles on barley (Hordeum vulgare L.) tissue culture. Biol Trace Elem Res 150(1–3):376–380. https://doi.org/10.1007/s12011-012-9480-z
Mhatre M (2007) Micropropagation of pineapple, Ananas comosus (L.) Merr. In: Jain SM, Häggman H (eds) Protocols for micropropagation of Woody trees and fruits. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6352-7_45
Nasirujjaman K, Uddin MS, Zaman S, Reza MA (2005) Micropropagation of turmeric (Curcuma longa Linn.) through in vitro rhizome bud culture. J Biol Sci 5(4):490–492. https://doi.org/10.3923/jbs.2005.490.492
Padmavathy N, Vijayaraghavan R (2008) Enhanced bioactivity of ZnO nanoparticles—an antimicrobial study. Sci Technol Adv Mater 9(3):035004. https://doi.org/10.1088/1468-6996/9/3/035004
Plaksenkova I, Kokina I, Petrova A, Jermaļonoka M, Gerbreders V, Krasovska M (2020) The impact of zinc oxide nanoparticles on cytotoxicity, genotoxicity, and MiRNA expression in barley (Hordeum vulgare L.) seedlings. https://doi.org/10.1155/2020/6649746. The Scientific World Journal
Rupasinghe RADS, Jayasinghe HASL, Rathnayake RMPS, Silva TAP (2016) Determinants for contribution of pineapple growers for export volume in Gampaha district in Sri Lanka. Curr Agric Res J 4(1):69. https://doi.org/10.12944/CARJ.4.1.07
Safavi K (2014) Effect of titanium dioxide nanoparticles in plant tissue culture media for enhance resistance to bacterial activity. Bull Environ Pharmacol Life Sci 3(5):163–166. http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1066.9008&rep=rep1&type=pdf
Science Buddies (2012) Interpreting Plates. https://www.sciencebuddies.org/science-fair-projects/references/interpreting-agar-plates. Accessed 20 April 2021
Sirelkhatim A, Mahmud S, Seeni A, Kaus NHM, Ann LC, Bakhori SKM, Hasan H, Mohamad D (2015) Review on zinc oxide nanoparticles: antibacterial activity and toxicity mechanism. Nano-micro Lett 7(3):219–242. https://doi.org/10.1007/s40820-015-0040-x
Xu X, Chen D, Jiang YZ, Wang M, Zhou L, Fan Z, Wang X Y and, Hui D (2013) Antimicrobial mechanism based on H2O2 generation at oxygen vacancies in ZnO crystals. Langmuir 29(18):5573–5580. https://doi.org/10.1021/la400378t
Yamamoto O (2001) Influence of particle size on the antibacterial activity of zinc oxide. Int J Inorg Mater 3(7):643–646. https://doi.org/10.1016/S1466-6049(01)00197-0
Zakharova OV, Gusev AA (2019) Photocatalytically active zinc oxide and titanium dioxide nanoparticles in clonal micropropagation of plants: prospects. Nanotechnol Russia 14:311–324. https://doi.org/10.1134/S1995078019040141
Zhiyuan L, Shuili Y, Heedeung P, Qingbin Y, Guicai L, Qi L (2016) Impact of titanium dioxide nanoparticles on the bacterial communities of biological activated carbon filter intended for drinking water treatment. Environ Sci Pollut Res 23(15):15574–15583. https://doi.org/10.1007/s11356-016-6742-x
Jahan S, Alias YB, Bakar AFBA, Yusoff IB (2018) Toxicity evaluation of ZnO and TiO2 nanomaterials in hydroponic red bean (Vigna angularis) plant: physiology, biochemistry and kinetic transport. J Environ Sci 72:140–152. https://doi.org/10.1016/j.jes.2017.12.022
Author Information
Department of Biotechnology, Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Gonawila (NWP), Makandura, Sri Lanka