Electrophoretic Characterization of the Cowpea Mutants for Seed Storage Proteins [Vigna unguiculata (L.)Walp]

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Research Article | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/2229-4473.2018.00104.0
First Page: 126
Last Page: 129
Views: 1723


Keywords: Electrophoretic characterization, cow pea, seed, storage protein, Vigna unguiculata


Abstract

An investigation was carried out on 38 EMS derived M6 generation mutants of two cowpea varieties RC-19 and RC-101 to determine their seed storage protein subunit profile variation through Sodium Dodecyl Sulphate - Polyacrylamide Gel Electrophoresis (SDS-PAGE). These mutants have deviated from their respective parents for their seed coat color, size and shape. The proteins extracted from cotyledons represented both water and salt soluble proteins. The results of the SDS-PAGE revealed 18 protein subunit bands in the range of 97.4 kD to ~14 kD molecular weight (MW). The protein bands could invariably be placed in five distinct regions. Only certain bands of region III and IV were polymorphic. The binary data generated from the polymorphic bands over the genotypes ( parents and mutants) were used to compute Jaccard’s similarity coefficients using NTSYS-pc software. The similarity matrix thus prepared was used to construct a dendrogram by UPGMA. The dendrogram distributed the 30 mutants of RC 101 and 8 mutants of RC 19 into 11 and 5 minor clusters, respectively. However, for both group of mutants two major clusters were apparent. One mutant in each group assumed unique place. The cotyledonary protein content of the mutants was invariably reduced as compared to their parents and ranged from 21-30.3 %.

Electrophoretic characterization, cow pea, seed, storage protein, Vigna unguiculata


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References

  1. Egbadzor  KP,  Yaboah  M,   Gamedoagbal  DK,  Offei,  SK, Danqual  EY  and Ofori K  (2014). Inheritance of seed coat color in cowpea [Vigna unguiculata (L.) Walp]. Int  J  Plant  Breed   Genet  8(1): 35-43.
  2. Girija M,  Dhanavel,  D  and  Gnanamurthy  S  (2013). Gamma rays and EMS induced flower color and seed mutants in cowpea [Vigna unguiculata (L.) Walp]. Adv  Appl   Sci  Res   4(2):134-139.
  3. Hammed  A, Saddiqa  A,  Nadeem S,  Iqbal V,  Atta  BM  and  Shah TM (2012). Genotypic and mutant identification in cicer arietinum by seed storage protein profiling. Pak  J  Bot   44 (4): 1303-1310.
  4. Jaccard  P (1908). Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles   44: 223-270.
  5. Laemmli  UK (1970). Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature  227: 680 – 685.
  6. Mehrotra R,   Kumar S,  Mehrotra S and Sing  BD (2008). Seed storage protein gene regulation. Ind   J  Biotech  8:147-158.
  7. Odeigah  PGC   and Osanyinpeju  AO (1998). Evaluating the genetic biodiversity of Bambara groundnut accessions from Nigeria using SDS-PAGE.  Biol  Sci  University of  Lags  45 (5): 451-458.
  8. Ram  Hari Har (2012). Vegetable Breeding : Principles & Practices, 3rd Revised Enlarged Edition. Kalyani Publishers, New Delhi, pp: 463-464.
  9. Reda M  Gaafar,  Marwa Hamouda,   Abdelfattah  B. (2016). Seed coat color, weight and eye pattern inheritance in gamma-rays induced cowpea M2-mutant line.  J   Genet   Engg   Biotech   14 : 61–68
  10. Rohlf  FJ (2000). NTSYS-pc: Numerical Taxonomy System. Ver. 2.1. Exeter Software, Setauket, NY, USA. 29-34.
  11. Singh  VV,  Ram Krishna  K  and Arya  RK (2006). Induced chemical mutagenesis in cowpea (Vigna unguiculata (L.) Walp.). Ind   J   Genet  66 (4): 312-315.
  12. Sharma  DB  and K  Ram Krishna (2017). Genetic diversity in cowpea [Vigna unguiculata (L.) Walp.] accessions using protein profiling. Int  J  Pure  App  Biosci  5(2): 491- 496.
  13. Tchiagam  JBN,   Bell  JM,  Nassourou  AM,  Njintang  NY   and Youmbi  E  (2011). Genetic analysis of seed proteins contents in cowpea (Vigna unguiculata L. Walp.). Afri  J  Biotech  10(16):  3077-3086.
  14. Tripathy  SK,  Sardar  SS  and Mishra  PK (2010). Analysis of seed storage protein pattern: a  method for studying genetic variation and diversity among  Vigna  genotypes.  Ind  J  Genet  70(2): 140-144.  
  15. Yap  IV  and Nelson  RJ  (1996). WinBoot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. IRRI Disc. Ser. No. 14. I.R.R.I, Manila, Philippines.

 


Acknowledgements


Author Information

Madhu Choudhary
Department of Plant Breeding and Genetics, SKN College of Agriculture, SKN Agricultural University, Jobner 303329 (Jaipur), Rajasthan, India ,

K . Ram Krishna*
Department of Plant Breeding and Genetics, SKN College of Agriculture, SKN Agricultural University, Jobner 303329 (Jaipur), Rajasthan, India ,
kramkrishna4@gmail.com

Vijay Veer Singh
Directorate of Rapeseed & Mustard Research, Sewar, Bharatpur, Rajasthan