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Bisen Mansi Singh, Tiwari Supriya, Agrawal Shashi Bhushan, Shiddamallayya N., Arya Jagdish Chandra, Gaur Sugriv Kumar, Pandey-Rai Shashi
Keywords: Salparni, Secondary metabolites, Phytochemicals, In vitro propagation, Hydroponics, Elicitation, Secondary metabolite enhancement, Ayurvedic pharmaceutics
Desmodium gangeticum (“Salparni”) is a pharmacologically significant Ayurvedic plant rich in bioactive secondary metabolites. However, industrial up-scaling is hindered by an over reliance on wild populations and unoptimized cultivation workflows, while existing literature details its phytochemistry and pharmacology, a comprehensive framework integrating traditional applications with modern metabolic and culture optimization is lacking. This review addresses this gap by evaluating phytochemical; diversity, therapeutic applications, and advanced biotechnological cultivation strategies specifically in-vitro propagation, hydroponics, and elicitor-mediated secondary metabolite enhancement. Furthermore, the molecular and genetic mechanisms regulating gene expression and metabolic pathways that govern biomass and bioactive compound accumulation have been been emphasized. By providing actionable insights into scalable standardized production, the review outlines the development of reliable agro-industrial value chains to maximize the commercial and pharmacological potential of D. gangeticum.
Alahmad S, Dinglasan E, Leung KM, Riaz A, Derbal N, VossFels KP, Able JA, Bassi FM, Christopher J, Hickey LT (2018) Speed breeding for multiple quantitative traits in durum wheat. Plant Methods 14(1):1–15
Alqahtani YS, Chidrawar VR, Shiromwar S, Singh S, Maheshwari R, Chitme H, Chilamakuru NB, Mohite P, Aljameeli AM, Khateeb MM (2024) A multi-modal approach to investigate Desmodium gangeticum’s influence on stress-induced male infertility: in vivo, in vitro, and in silico assessments. Biomed Pharmacother 173:116358. https://doi.org/10.1016/j.biopha.2024.116358
Aparna S, Ved DK, Lalitha S, Venkatasubramanian P (2012) Botanical identity of plant sources of Daśamūla drugs through an analysis of published literature. Anc Sci Life 32(1):3–10
Armstrong W (1980) Aeration in higher plants. Adv Bot Res 7:225–332
Azani N, Babineau M, Bailey CD, Banks H, Barbosa AR, Pinto RB, Boatwright JS, Borges LM, Brown GK, Bruneau A, Candido E, Cardoso D, Chung F, Clark RP, Conceição S, Crisp M, Cubas P, Delgado-Salinas A, Dexter KG (2017). A new subfamily classification of the Leguminosae based on a taxonomically comprehensive phylogeny: the Legume Phylogeny Working Group (LPWG). TAXON, 66(1): 44–77. https://doi.org/10.12705/661.3
Benke K, and Tomkins B (2017). Future food-production systems: vertical farming and controlled-environment agriculture. Sustainability: Science, Practice and Policy, 13(1), 13–26. https://doi.org/10.1080/15487733.2017.1394054
Bernardo R (2016) Bandwagons I, too, have known. Theo Appl Genet 129:2323–2332. https://doi.org/10.1007/S00122-016-2772-5
Bernardo R, Yu J (2007) Prospects for genomewide selection for quantitative traits in maize. Crop Sci 47(3):1082–1090. https://doi.org/10.2135/cropsci2006.11.0690
Bhattacharjee A, Shastry S, and Saha S (2013) Phytochemical and ethno-pharmacological profile of D. gangeticum (L.) DC : A review. Int J Biomed Res, 4(10): 507 https://doi.org/10.7439/ijbr.v4i10.355
Bleß W, Barz W (1988) Isolation of pterocarpan synthase, the terminal enzyme of pterocarpan phytoalexin biosynthesis in cell suspension cultures of Cicer arietinum. FEBS Lett 235(1–2):47–50
Bonnecarrere V, Rosas J, Ferraro B (2019) Economic impact of marker-assisted selection and rapid generation advance on breeding programs. Euphytica 215:197. https://doi.org/10.1007/s10681-019-2529-8
Bosland PW, Barchenger DW (2023) Resistance: classical breeding methods. Breed Dis-Resistant Horticultural Crops. https://doi.org/10.1016/B978-0-443-15278-8.00008-5
Brim CA (1966) A modified pedigree method of selection in soybeans 1. Crop Sci 6(2):220–220
Crossa J, Pérez P, Hickey J et al (2014) Genomic prediction in CIMMYT maize and wheat breeding programs. Heredity 112(48):60. https://doi.org/10.1038/hdy.2013.16
Daniel M and Purkayastha RP (1995) Handbook of Phytoalexin Metabolism And Action (Hardbound - 1995) ISBN13 9780824792695
Daniel M and Purkayastha RP (1995) Progress in Phytoalexins Research during the Past 50 Years. Handbook of Phytoalexin Metabolism and Action, 3
Daniel M, Patel J, Patel N, and Pathak D (2022) D. gangeticum (L.) DC. World J Pharmaceutical Life Sci. https://doi.org/10.20959/wjpps202210-23423
Das A, Singh S, Islam Z, Munshi AD, Behera TK, Dutta S, Weng Y, Dey SS (2022) Current progress in genetic and genomics-aided breeding for stress resistance in cucumber (Cucumis sativus L.). Sci Hortic 300:111059. https://doi.org/10.1016/j.scienta.2022
Das M, Molla SA, Debnath S, Mondal S, Gurav A, Meena AK, Babu G (2025) A brief review on chemoprofling and pharmacological potential of Desmodium gangeticum (L) DC. Nat Prod Res. https://doi.org/10.1080/14786419.2025.2455458
De La Fuente J, Amate J, Carmen M, Artuch R, Manuel J, Fadda S (2020) Effects of levels of self-regulation and regulatory teaching on strategies for coping with academic stress in undergraduate students. Front Psychol 11:494751. https://doi.org/10.3389/fpsyg.2020.00022
Dorais, M., and Dubé, Y. (2009). Managing greenhouse organic wastes: a holistic approach. In International Symposium on High Technology for Greenhouse Systems: GreenSys2009 893 183–197
Dutta S, Muthusamy V, Chhabra R, Zunjare RU, Hossain F (2019a) Characterization of diverse provitamin: A rich inbreds for lipoxygenase (LOXs) gene governing oxidative deterioration in maize. IJCS 7(5):841–846
Dutta S, Muthusamy V, Zunjare RU, Hossain F (2019b) Analysis of paralogous genes of carotenoid dioxygenase affecting carotenoid biosynthesis pathway in maize (Zea mays L.). J Pharmacognosy Phytochem. 8(2):524–530
Dutta S, Muthusamy V, Hossain F, Baveja A, Chhabra R, Jha SK, Yadava DK, Zunjare RU (2020) Analysis of genetic variability for retention of kernel carotenoids in sub-tropically adapted biofortified maize under different storage conditions. J Cereal Sci 93:102987
Dutta S, Muthusamy V, Chhabra R, Baveja A, Zunjare RU, Mondal TK, Yadava DK, Hossain F (2021) Low expression of carotenoids cleavage dioxygenase 1 (ccd1) gene improves the retention of provitamin-A in maize grains during storage. Mol Genet Genomics 296(1):141–153
Fernandes LP, Shenoy A, Shabaraya AR (2019) A review on pharmacological activities of Desmodium gangeticum. Int J Pharma Chem Res 5(2):96
Fisher C, Ebenau-Jehle and Grisebach H (1990) Purification and characterization of pterocarpan synthase from elicitor-challenged soybean cell cultures. Phytochemistry 29:2879–2882
Frisch M, Melchinger AE (2005) Selection theory for marker-assisted backcrossing. Genetics 170(2):909–917
Gaba Y, Pareek A, Singla-Pareek SL (2021) Raising climate-resilient crops: journey from the conventional breeding to new breeding approaches. Curr Genomics 22:450–467. https://doi.org/10.2174/1389202922666210928151247
Gaur PM, Samineni S, Gowda CLL and Rao BV (2007) Rapid generation advancement in chickpea. Journal of SAT Agricultural Research. 3(1):1–3
Ghosh D, Anandakumar A (1983) Anti-inflammatory and analgesic activities of Gangetin-A Pterocarprnoid from Desmodium gangeticum. Indian J Pharmacology 15(4):391–402
Ghosh S, Watson A, Gonzalez-Navarro OE, Ramirez-Gonzalez RH, Yanes L, Mendoza-Suárez M, Simmonds J, Wells R, Rayner T, Green P, Hafeez A (2018) Speed breeding in growth chambers and glasshouses for crop breeding and model plant research. Nat Protoc 13(12):2944–2963
Gopinath P, Vethamoni PI, Gomathi M (2017) Aeroponics soilless cultivation system for vegetable crops. Chem Sci Rev Lett 6(22):838–849
Goto E, Both AJ, Albright LD, Langhans RW, and Leed AR (1996) Effect of dissolved oxygen concentration on lettuce growth in floating hydroponics. In international symposium on plant production in closed ecosystems 440:205–210
Govindarajan R, Rastogi S, Vijayakumar M, Shirwaikar A, Rawat AKS, Mehrotra S, Pushpangadan P (2003) Studies on the antioxidant activities of Desmodium gangeticum. Biol Pharm Bull 26:1424–1427
Govindarajan R, Vijayakumar M, Shirwaikar ANNIE, Rawat AKS, Mehrotra S, Pushpangadan P (2006) Antioxidant activity of D gangeticum and its phenolics in arthritic rats. ACTA PHARMACEUTICA-ZAGREB- 56(4):489
Govindarajan R, Asare-Anane H, Persaud S, Jones P, Houghton PJ (2007a) Effect of D. gangeticum extract on blood glucose in rats and on insulin secretion in vitro. Planta Med 73:427–432
Govindarajan R, Vijayakumar M, Rao CV, Shirwaikar A, Kumar S, Rawat AKS, Pushpangadan P (2007b) Antiinflammatory and antioxidant activities of D. gangeticum fractions in carrageenan-induced inflamed rats. Phytother Res 21(10):975–979
Halder M, Sarkar S, Jha S (2019) Elicitation: a biotechnological tool for enhanced production of secondary metabolites in hairy root cultures. Eng Life Sci 19(12):880. https://doi.org/10.1002/elsc.201900058
Harborne JB (1977) Flavonoids and the evolution of the angiosperms. Biochem Syst Ecol 5(1):7–22
Heberle-Bors E (1989) Isolated pollen culture in tobacco: plant reproductive development in a nutshell. Sex Plant Reprod 2(1):1–10
Hooker JD (1973). JD 1872–1897. The Flora of British India. Vols. 1–7. Bishen Singh Mahendra Pal Singh, Dehra Dun, India.
Huang N, Angeles ER, Domingo J (1997) Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet 95:313–320. https://doi.org/10.1007/s001220050565
Ingham JL, Tahara S, Tahara S (1983) Isolation and identification of isoflavanone phytoalexins from leaflets of Diphysa robinioides. Zeitschrift Für Naturforschung C 38(11–12):899–904
Iwu MM, Jackson JE, Tally JD, Klayman DL (1992) Evaluation of plant extracts for antileishmanial activity using a mechanism-based radiorespirometric microtechnique (RAM). Planta Med 58:436–441
Jabbar S, Khan MT, Choudhuri MS (2001) The effects of aqueous extracts of D. gangeticum DC. (Leguminosae)on the central nervous system. Pharmazie 56:506–508
Jabbour F, Gaudeul M, Lambourdiere J, Ramstein G, Hassanin A, Labat JN, Sarthou C (2018) Phylogeny, biogeography and character evolution in the tribe Desmodieae (Fabaceae: Papilionoideae), with special emphasis on the New Caledonian endemic genera. Mol Phylogenet Evol 118:108–121. https://doi.org/10.1016/j.ympev.2017.09.017
Jagadish SV, Bahuguna RN, Djanaguiraman M, Gamuyao R, Prasad PV, Craufurd PQ (2018) Implications of high temperature and elevated CO2 on flowering time in plants. Front Plant Sci 7:913. https://doi.org/10.3389/fpls.2016.00913
Jena KK, Mackill DJ (2008) Molecular markers and their use in marker-assisted selection in rice. Crop Sci 48:1266–1276. https://doi.org/10.2135/cropsci2008.01.0020
Jin DP, Choi IS, Choi BH (2019) Plastid genome evolution in tribe Desmodieae (Fabaceae: Papilionoideae). PLoS ONE 14(6):e0218743. https://doi.org/10.1371/journal.pone.0218743
Joshi HandParle M (2006) Antiamnesic effects of Desmodium gangeticum in mice. Yakugaku Zasshi 126(9):795–804
Joshi BR, Hakim MM, Patel IC (2023) The biological active compounds and biological activities of Desmodium species from Indian region: a review. Beni-Suef University J Basic Appl Sci 12(1):1. https://doi.org/10.1186/s43088-022-00339-4
Keller EJ, Korzun L (1996) Ovary and ovule culture for haploid production. In Vitro Haploid Production in Higher Plants: Volume 1—Fundamental Aspects and Methods. Springer, Netherlands, Dordrecht, pp 217–235
Khush GS (1989). Innovative approaches to rice improvement. In Rice Farming Systems: New Directions: Proceedings of an International Symposium 31 January-3 February 1987, Rice Research and Training Center, Sakha, Egypt (Vol. 4, No. 7.8, p. 71). Int. Rice Res. Inst.
Kigoni M, Choi M, Arbelaez JD (2023) ‘Single-seed-speed bulks:’ a protocol that combines ‘speed breeding’ with a cost-efficient modified single-seed descent method for rapid-generation-advancement in oat (Avena sativa L). Plant Methods 19:92. https://doi.org/10.1186/s13007-023-01067-1
Kirtikar KR, Basu BD (1987) Indian Medicinal Plants. Lalit Mohan Basu, M.B. 49, Leader Road, Allahabad, India. Vols. 1–4
Klerkx L, Rose D (2020) Dealing with the game-changing technologies of Agriculture 4.0 How do we manage diversity and responsibility in food system transition pathways? Glob Food Secur 24:100347. https://doi.org/10.1016/j.gfs.2019.100347
Kondić-Špika A, Kobiljski B, Hristov N (2008) Efficiency of anther culture technique in the production of wheat double haploids. Zbornik Matice Srpske Za Prirodne Nauke 115:35–40
Kumar M, Hussaini MK, Samiulla SA, Kishore Kumar L, KK Khan, SA, Aminuddin A, and Jamil SS (2012). Ethnomedicinal observations from the Anant prasad and Jeypur tribal villages of district Cuttack, Odisha, India. Journal of Applied Pharmaceutical Science 2(11):127–130. https://doi.org/10.7324/JAPS.2012.21123
Kurian GA and Paddikkala J (2009) Administration of aqueous extract of D. gangeticum (L) root protects rat heart against ischemic reperfusion injury induced oxidative stress. Indian J Exp Biol 47:129–135
Kurian GA and Paddikkala J (2010) Oral delivery of insulin with D. gangeticum root aqueous extract protects rat hearts against ischemia reperfusion injury in streptozotocin induced diabetic rats. Asian Pac J Tropical Med 3(2):94–100
Kurian GA, Philip S, Varghese T (2005) Effect of aqueous extract of the D. gangeticum DC root in the severity of myocardial infarction. J Ethnopharmacology 97(3):457–461
Kurian GA, Rajamani T, Ramanarayanan P, Paddikkala J (2009a) A comparative study on invitro and in vivo antioxidant activities of aqueous extract of D. gangeticum (Leguminosae)root. Int J Green Pharm 3:324–331
Kurian GA, Selvam GS and Vidyasagar Saranya VS (2009b) Oral administration of insulin mixed D. gangeticum root aqueous extract reduces oxidative stress mediated by myocardial ischemia reperfusion in isolated rat hearts
Latha P, Govindasamy S (1997) Gangetin—a reproductive inhibitor in male rats. Phytother Res 11:466–468
Latha P, Govindasamy S, Balakrishna K (1997) Effect of gangetin on fertility of male rats. Phytother Res 11:372–375
Laux P, Jäckel G, Tingem RM, Kunstmann H (2010) Impact of climate change on agricultural productivity under rainfed conditions in cameroon—a method to improve attainable crop yields by planting date adaptations. Agric for Meteorol 150(9):1258–1271
Ma L, Kong F, Sun K, Wang T, Guo T (2021) From classical radiation to modern radiation: past, present, and future of radiation mutation breeding. Front Public Health 9:768071. https://doi.org/10.3389/fpubh.2021.768071
Mahajan K, Kumar D, Kumar S (2015) Antiamnesic activity of extracts and fraction of Desmodium gangeticum. J Pharmaceutical Technol, Res Manag 3(1):67–77
Mairapetyan S, Alexanyan J, Tovmasyan A, Daryadar M, Stepanian B, Mamikonyan V (2016) Productivity, biochemical indices and antioxidant activity of peppermint (Mentha piperita L.) and basil (Ocimum basilicum L.) in conditions of hydroponics. J. Aquac. Res. Dev 7(6):1–3
Meuwissen THE, Hayes BJ & Goddard ME (2001). Prediction of total genetic value using genome-wide dense marker maps. Genetics, 157(4), 1819–1829. https://www.genetics.org/content/157/4/1819
Mobini SH, Lulsdorf M, Warkentin TD, Vandenberg A (2015) Plant growth regulators improve in vitro flowering and rapid generation advancement in lentil and faba bean. In Vitro Cell Developmental Biol-Plant 51(1):71–79
Muzaffer A, Pillai NR, Purushothaman AK (1982) Examination of biochemical parameter after administration of gangetin in female albino rats. J Res Ayurveda Sidha 2:172–175
Niranjan A, Tewari SK (2008) Phytochemical composition and antioxidant poten tial of D. gangeticum (Linn.) DC. Nat Prod Radiance 7:35–39
Niu G, Masabni J (2021) Hydroponics. Plant Fact Basics, Applications Adv,. https://doi.org/10.1016/B978-0-323-85152-7.00023-9
Ortiz R, Trethowan R, Ferrara GO, Iwanaga M, Dodds JH, Crouch JH, Crossa J, Braun HJ (2007) High yield potential, shuttle breeding, genetic diversity, and a new international wheat improvement strategy. Euphytica 157:365–384. https://doi.org/10.1007/s10681-007-9375-9
Paiva NL, Edwards R, Sun Y, Hrazdina G, Dixon RA (1991) Stress responses in alfalfa (Medicago sativa L.) 11. Molecular cloning and expression of alfalfa isoflavone reductase, a key enzyme of isoflavonoid phytoalexin biosynthesis. Plant Mol Biol 17:653–667
Pandey MM, Rastogi S, Rawat AKS (2013) Indian traditional ayurvedic system of medicine and nutritional supplementation. Evidence-Based Complementary and Alternative Medicine 1:376327
Prasad MVV, Balakrishna K, Carey MW (2005) Hepatoprotective activity of roots of D. gangeticum (Linn.) DC. Asian J Chem 17:2847–2849
Rajaseger G, Chan KL, Tan KY, Ramasamy S, Khin MC, Amaladoss A, Haribhai PK (2023) Hydroponics: Current trends in sustainable crop production. Bioinformation 19(9):925. https://doi.org/10.6026/97320630019925
Rastogi S, Pandey MM, Rawat AKS (2011) An ethnomedicinal, phytochemical and pharmacological profile of D. gangeticum (L.) DC and Desmodium adscendens (Sw.) DC. J Ethnopharmacology 136(2):283–296. https://doi.org/10.1016/j.jep.2011.04.031
Rathi A, Rao C, Ravishankar B, De S, Mehrotra S (2004) Anti-inflammatory and anti-nociceptive activity of the water decoction Desmodium gangeticum. J Ethnopharmacol 95(2–3):259–263. https://doi.org/10.1016/j.jep.2004.07.009
Raviv M, Lieth JH, Bar-tal A, Silber A (2007) Growing plants in soilless culture: operational conclusions. Soilless Cult. https://doi.org/10.1016/b978-044452975-6.50015-0
Ritter E, Angulo B, Riga P, Herran C, Relloso J, San Jose M (2001) Comparison of hydroponic and aeroponic cultivation systems for the production of potato minitubers. Potato Res 44(2):127–135
Rosen CJ, Carlson RM (1984) Influence of root zone oxygen stress on potassium and ammonium absorption by Myrobalan plum rootstock. Plant Soil 80:345–353
Sagar MK, Aadesh Upadhyay AU, Kalpana K and Kumud Upadhyaya KU (2010). Evaluation of antinociceptive and anti-inflammatory properties of D. gangeticum (L.) in experimental animal models, 2: 33–43.
Laboratory of Morphogenesis Centre of Advance Study in Botany, Department of Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India