Enzymatic treatment improves ACE-I inhibiton and antiproliferative potential of chickpea

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00031-6
First Page: 363
Last Page: 369
Views: 1746


Keywords: ACE-I inhibitory activity, Chickpea seed proteins, Alcalase


Abstract

Chickpea seeds are the preferred source of proteins possessing health care functions in countries across the world. Study indicated the chickpea proteins as a promising center of bioactive peptides and open up new vista for food industry. Employing gastrointestinal enzyme alcalase, protein hydrolysates generated from 45 chickpea seed accessions were evaluated for angiotensin-I converting enzyme (ACE-I) inhibitory potential and antiproliferative influence. Alcalase at  1 h of optimum hydrolysis produced bioactive peptides inhibiting the ACE-I activity. The accession BDN-9-3 gave highest ACE-I inhibitory activity with IC50 value of 22.43 mg/ml. The protein hydrolysate of BDN-9-3 was further subjected to antiproliferative assessment against breast cancer cells MCF-7 and MDA-MB-231. The IC50 of BDN-9-3 alcalase hydrolysate was 0.60 mg/ml and 0.63 mg/ml in MCF-7 and MDA-MB-231 cells respectively, compared to non hydrolyzed chickpea protein (IC50 of 0.85 and 0.82 mg/ml). Present study ascertain that chickpea seed hydrolysate can be perceived as a valuable nutraceutical resource.


ACE-I inhibitory activity, Chickpea seed proteins, Alcalase


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Acknowledgements

The authors are thankful to the Hon’ble Vice-chancellor Prof. Sangeeta Shukla, Jiwaji University, Gwalior, India for providing research grant.


Author Information

Gupta Neha
School of Studies in Biotechnology, Jiwaji University, Gwalior, India

Bhagyawant Sameer Suresh
School of Studies in Biotechnology, Jiwaji University, Gwalior, India
sameerbhagyawant@gmail.com