Vegetos
(An International Journal of Plant Research & Biotechnology)
Establishment of an efficient in vitro regeneration protocol and successful acclimatization of sauromatum diversifolium (Wall. ex Schott) Cusimano & Hett
*Article not assigned to an issue yet
Purohit Vijay Kant, Maikhuri Rakesh Kumar, Kumar Rajeev Ranjan, Negi Nitin, Chauhan Jaidev, Dobhal Pradeep, Patni Babita, Trivedi Vijaylaxmi, Maikhuri Rakesh Kumar, Kumar Rajeev Ranjan
Short Communications | Published: 10 September, 2025
First Page: 0
Last Page: 0
Views: 4
Keywords: Nutraceutical, Micropropagation, Multiple shooting, IAA, TDZ, Rooting
Abstract
Sauromatum diversifolium, a lesser-known member of the Araceae family, possesses tubers with significant nutraceutical value and appetite-suppressing properties. In this study, an efficient protocol for micropropagation was developed using tuber explants with apical buds. Tuber bud cultured on Murashige and Skoog (MS) medium supplemented with thidiazuron (TDZ) in combination with gibberellic acid (GA₃) exhibited high shoot induction potential. Multiple shoot regeneration response of up to 95% was recorded, with the highest shoot proliferation exceeding 50 shoots per explant, observed at 10 µM TDZ combined with 20 µM GA₃ within 60 ± 5 days of inoculation. For in vitro rooting response, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) were tested. IAA showed superior rooting performance, with rooting percentages increasing in response to higher concentrations and up to 100% results within 50 ± 5 days of sub-culture. In contrast, high levels of IBA not only failed to induce rooting but also caused browning of the culture medium and inhibited shoot development. Acclimatization of the in vitro developed plantlets resulted in a survival rate of 65 ± 5%. These findings establish a robust and reproducible micropropagation protocol for S. diversifolium and open avenues for its mass propagation and nutraceutical exploration.
References
Ahmad N, Faisal M, Ahmad A, Alatar AA, Qahtan AA, Alok A (2022) Thidiazuron induced in vitro clonal propagation of Lagerstroemia speciosa (L.) Pers.—an important avenue tree. Horticulturae 8:359. https://doi.org/10.3390/horticulturae8050359
Chandola V, Chandra S, Nautiyal AR, Kumar RR, Purohit VK (2023) TDZ and zeatin induced in vitro shooting for efficient vegetative propagation in Trillium govanianum, an endangered alpine medicinal herb. Vegetos. https://doi.org/10.1007/s42535-023-00746-7
Chen J, Wei X (2018). Thidiazuron in micropropagation of aroid plants. Thidiazuron: from urea derivative to plant growth regulator. 95–113
Cusimano N, Barrett MD, Hetterscheid WLA, Renner SS (2010) A phylogeny of the areae (Araceae) implies that Typhonium, Sauromatum, and the Australian species of Typhonium are distinct clades. Taxon 59:439–447. https://doi.org/10.1002/tax.592009
Gopukumar ST, Alexander P, Fathima TKS, Jeniffa R, Praseetha PK (2015) Typhonium divaricatum: a promising endangered anti-cancer. Med. Plants Their Conserv. Manag., pp 114–119
Hartmann HT, Kester DE, Davies FT, Geneve RL (2011) Plant propagation: principles and practices, 8th edn. Pearson Prentice Hall
Kantaka S (2004) Colocasia esculenta (L.). Schott. In: Grubbrn GJH, Denton OA, Eds (eds) PROTA (Plant resources of tropical Africa / Ressourcesvegetales de l’ afrique tropicale). Netherlands, Wageningen
Liu H, Wen Y, Cui M, Qi X, Deng R, Gao J, Cheng Z (2020) Histological, physiological and transcriptomic analysis reveal gibberellin-induced axillary meristem formation in garlic (Allium sativum). Plants 9:970. https://doi.org/10.3390/plants9080970
Negi VS, MAikhuri RK, Vashishtha DP (2011) Traditional healthcare practices among the villages of Rawain valley, uttarkashi, uttarakhand, India. Indian J Traditional Knowl 10(3):533–537
Shiri M, Mudyiwa RM, Takawira M, Musara C, Gama T (2019) Effects of rooting media and indole-3-butyric acid (IBA) concentration on rooting and shoot development of Duranta erecta tip cuttings. Afr J Plant Sci 13(10):279–285
Singh V, Chauhan NS, Singh M, Idris A, Madanala R, Pande V, Mohanty CS (2014) Establishment of an efficient and rapid method of multiple shoot regeneration and a comparative phenolics profile in in vitro and greenhouse-grown plants of psophocarpus tetragonolobus (L.) DC. Plant Signal Behav 9(10). https://doi.org/10.4161/15592316.2014.970443. e970443
Souza JA, Bettoni JC, Dalla Costa M, Baldissera TC, dos, Passos JFM, Primieri S (2022) 3-IAA on in vitro rooting and acclimatization of apple rootstock. Communications 12:016–023
Start A (2017) Development of a typhonium sp.(Araceae) from the East Kimberley, Western Australia. J R Soc West Aust 100(1):9–17
Vaneker K, Slaats E (2012) AROIDS: the world’s oldest food crop. Accessed 30/03/2024. http://www.b4fn.org/fileadmin/B4FN_Docs/documents/Case_study_documents/Aroids_factsheet.pdf
Wawrosch C, Zeitlhofer P, Grauwald B, Kopp B (2001) Effects of rooting chemicals on the establishment of micropropagated Picrorhiza Kurroa plantlets in the greenhouse. Acta Hort 616:271–274. https://doi.org/10.17660/ActaHortic.2003.616.37
Zheng C, Kwame Acheampong A, Shi Z, Halaly T, Kamiya Y, Ophir R, Galbraith DW, Or E (2018) Distinct gibberellin functions during and after grapevine bud dormancy release. J Exp Bot 69:1635–1648. https://doi.org/10.1093/jxb/ery022
Jadhao AS, Shaikh FT, Sirsat DR, Bathe PN, Agrawal NK (2024) Phytochemical Screening of Sauromatum venosum (Ait.) Schott,(Araceae). Multi-Disciplinary Research. Volume 1 Lambert Academic Publishing.
Author Information
High Altitude Plant Physiology Research Center, H.N.B. Garhwal University, Srinagar, India