Evaluation of genoprotective and antioxidative potentiality of ethanolic extract of N. sativa seed in streptozotocin induced diabetic albino rats

, ,

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00201-5
First Page: 453
Last Page: 459
Views: 647

Keywords: Comet assay, DNA damage, Nigella sativa , Antioxidant


In the present study oxidative stress, DNA damage in streptozotocin induced diabetic rats and its repair after treating with ethanolic extract of Nigella sativa seed were evaluated via comet assay and estimation of SOD and TBARS. Blood lymphocytes of Streptozotocin (60 mg/kg BW) induced diabetic rats showed significant increase (p < 0.05) in DNA damage as evidenced by comet tail length, due to the formation of reactive oxygen species (ROS) leading to genotoxicity. Feeding rats with ethanolic extract of N. sativa seed (500 mg/kg BW) significantly (p < 0.05) improved the level of SOD and TBARS and decreased the comet tail length and percentage of comet cells, indicating the antioxidative and genoprotective potentiality of Nigella sativa seed.

Comet assay, DNA damage, 
                Nigella sativa
              , Antioxidant

*Pdf Download

(*Only SPR Members can download pdf file; #Open Access;)


  1. Akbarzadeh A, Norouzian D, Mehrabi MR, Jamshidi Sh, Farhangi A, Allah Verdi A, Mofidian SMA, Lame Rad B (2007) Induction of diabetes by Streptozotocin in rats. Indian J Clin Biochem 22(2):60–64

  2. Al Wafai RJ (2013) Nigella sativa and thymoquinone suppress cyclooxygenase-2 and oxidative stress in pancreatic tissue of streptozotocin-induced diabetic rats. Pancreas 42(5):841–849

  3. Alam P, Yusufoglu H, Alam A (2013) HPTLC densitometric method for analysis of thymoquinone in Nigella sativa extracts and marketed formulations. Asian Pac J Trop Dis 3(6):467–471

  4. Ali BH, Blunden G (2003) Pharmacological and toxicological properties of Nigella sativa. Phytother Res 17:299–305

  5. Babazadeh B, Sadeghnia HR, Kapurchal ES, Nasri S, Najaran ZT (2012) Protective effect of Nigella sativa and thymoquinone on serum/glucose deprivation-induced DNA damage in PC12 cells. Avicenna J Phytomed 2(3):125–132

  6. Badary OA (1999) Thymoquinone attenuates ifosfamide-induced Fanconisyndromein rats and enhances its antitumor activity in mice. J Ethnopharmacol 67:135–142

  7. Badary OA, Taha RA, Gamal el-Din AM, Abdel-Wahab MH (2003) Thymoquinone Is a potent superoxide anion scavenger. Drug Chemical Toxicol 26:87–98

  8. Biswas M, Kar B, Bhattacharya S, Kumar RB, Ghosh AK, Haldar PK (2011) Antihyperglycemic activity and antioxidant role of Terminalia arjuna leaf in streptozotocin-induced diabetic rats. Pharm Biol 49(4):335–340

  9. Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytother Res 14:323–328

  10. Damasceno DC, Netto AO, Iessi IL, Gallego FQ, Corvino SB, Dallaqua B, Sinzato YK, Bueno A, Calderon IPM, Rudge MVC (2014) Streptozotocin-induced diabetes models: pathophysiological mechanisms and fetal outcomes. Biomed Res Int 2014(2014):819065

  11. Datta NJ, Namasivayam A (2003) In vitro effect of methanol on folate-deficient rat hepatocytes. Drug Alcohol Depend 71:87–91

  12. David MN (1996) The pathophysiology of diabetic complications. Ann Intern Med 124:86–89

  13. El-Tantawy WH, Temraz A (2018) Management of diabetes using herbal extracts: review. Arch Physiol Biochem 124(5):383–389

  14. Erejuwa OO, Sulaiman SA, Wahab MSA, Sirajudeen KNS, Salzihan M, Salleh M, Gurtu S (2010) Antioxidant protective effect of Glibenclamide and Metformin in combination with honey in pancreas of streptozotocin-induced diabetic Rats. Int J Mol Sci 11(5):2056–2066

  15. Fabricant DS, Farnsworth NR (2001) The value of the plants used in traditional medicine for drug discovery. Environ Health Perspect 109:69–75

  16. Fiorentino TV, Prioletta A, Zuo P, Folli F (2013) Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases. Curr Pharm Des 19(32):5695–5703

  17. Gilhotra N, Dhingra D (2011) Thymoquinone produced antianxiety-like effects in mice through modulation of GABA and NO levels. Pharmacol Rep 63:660–669

  18. Hossein H, Siavash P, Marjan NA, Sadeghnia Hamid R, Toktam Z (2007) Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cerebral ischemia-reperfusion injury in rat hippocampus. Phytomedicine 14:621–627

  19. Houghton PJ, Zarka R, De las Heras B, Hoult JR (1995) Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 61:33–36

  20. Ilhan A, Gurel A, Armutcu F, Kamisli S, Iraz M (2005) Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazol-induced kindling in mice. Neuropharmacology 49:456–464

  21. Isabelle M, Michel P, Dominique L (2005) Antioxidant strategies in the treatment of stroke. Free Radical Biol Med 39:429–443

  22. Janusz B, Michal A, Renata K, Katarzyna W, Marek Z et al (2004) DNA damage and repair in type 2 diabetes mellitus. Mutat Res 554:297–304

  23. Kaatabi H, Bamosa AO, Badar A, Al-Elq A, Abou-Hozaifa B, Lebda F, Al-Khadra A, Al-Almaie S (2015) Nigella sativa improves glycemic control and ameliorates oxidative stress in patients with type 2 Diabetes Mellitus: placebo controlled participant blinded clinical trial. PLoS ONE 10(2):e0113486

  24. Kaneto NY, Kawamori D, Miyatsuka T, Matsuoka TA, Matsuhisa M, Yamasaki Y (2006) Role of oxidative stress, endoplasmic reticulum stress, and c-Jun N-terminal kinase in pancreatic beta-cell dysfunction and insulin resistance. Int J Biochem Cell Biol 38(5–6):782–793

  25. Kanter M, Coskun O, Kalayci M, Buyukbas S, Cagavi F (2006) Neuroprotective effects of Nigella sativa on experimental spinal cord injury in rats. Hum Exp Toxicol 25:127–133

  26. Kassie F, Parzefall W, Knasmüller S (2000) Single cell gel electrophoresis assay: a new technique for human biomonitoring studies. Mutat Res 463:13–31

  27. Kedziora-Kornatowska K, Szewczyk-Golec K, Kozakiewicz M, Pawluk H, Czuczejko J, Kornatowski T et al (2009) Melatonin improves oxidative stress parameters measured in the blood of elderly type 2 diabetic patients. J Pineal Res 46:333–337

  28. Kumar GPS, Arulselvan P, Kumar SD, Subrimaniam PS (2006) Antidiabetic activity of fruits of Terminalia chebulaon STZ induced diabetic rats. J Health Sci 52:283–291

  29. Lenzen S (2008) The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia 5:216–226

  30. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47(3):469–474

  31. Kanter M (2008) Effects of Nigella sativa and its major constituent, Thymoquinone on sciatic nerves in experimental diabetic neuropathy. Neurochem Res 33:87–96

  32. Mousavi SH, Tayarani-Najaran Z, Asghari M, Sadeghnia HR (2010) Protective effect of Nigella sativa extract and Thymoquinone on serum/glucose deprivation-induced PC12 cells death. Cell Mol Neuro biol 30:591–598

  33. Nehar S, Kumari M (2013) Ameliorating Effect of Nigella sativa oil in thioacetamide-induced liver cirrhosis in albino rats. Indian J Pharm Educ Res 47(2):135–139

  34. Nehar S, Rani P (2011) HPTLC studies on ethanolic extract of Nigella sativa Linn. seeds and its phytochemical standardization. Ecoscan 1:105–108

  35. Nichans WG, Samuelson B (1968) Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 6:126–130

  36. Pavana P, Sethupathy S, Manoharan S (2007) Antihyperglycemic and antilipidperoxidative effects of Tephrosia purpurea seed extract in streptozotocin induced diabetic rats. Indian J Clin Biochem 22:77–83

  37. Rajkamal G, Suresh K, Sugunadevi G, Vijayaanand MA, Rajalingam K (2010) Evaluation of chemopreventive effects of Thymoquinone on cell surface glycoconjugates and cytokeratin expression during DMBA induced hamster buccal pouch carcinogenesis. BMB Rep 43(10):664–669

  38. Salem ML (2005) Immunomodulatory and therapeutic properties of the Nigella sativa L seed. Int Immunopharmacol 5:1749–1770

  39. Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191






The author is grateful to the University Grant Commission, New Delhi, for providing financial assistance by granting major research project. (No. 37-325/2009 SR).

Author Information

Nehar Shamshun
Ranchi University, Ranchi, India
Rani Prabha
Ranchi University, Ranchi, India

Kumar Chandan
Government RRM P.G. College, Surajpur, India