Evaluation of phytochemical components, antioxidant and antibacterial activities of silver nanoparticles synthesized using Ricinus communis leaf extracts


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00244-8
First Page: 606
Last Page: 618
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Keywords: Antibacterial, Antioxidant, Phytochemicals, Ricinus communis , Silver nanoparticles


Silver nanoparticles (AgNPs) are metal particles with nanoscale size ranging from 1 to 100 nm. Due to their small size, they can be used in different fields such as medicine, environmental protection, drug delivery, gene therapy, food processing etc. As necessary phytochemicals such as flavonoids, tannins and triterpenes were present in Ricinus communis leaf, its aqueous extract was used in the present study to biosynthesize AgNPs. A colour change from colourless to golden yellow, and then to brown colour after 100 min incubation of “leaf extract plus AgNO3 reaction mixture” in the dark confirmed the formation of AgNPs. Absorption spectrum of AgNPs was studied between 400 and 700 nm and AgNPs were found to have absorption maximum at 440 nm. Binding of phenolic and flavonoids compounds with silver was confirmed by measuring their content in AgNPs. The antioxidant potential of synthesized AgNPs was evaluated by DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging assay. Antioxidant assay showed that AgNPs had significantly higher DPPH radical scavenging activity (60.54%) than the aqueous leaf extract (44.49%) of R. communis at P < 0.05. The antibacterial activity of AgNPs against a Gram-positive (Staphylococcus aureus) and two Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria was evaluated by cup-plate agar diffusion method. R. communis leaf extract synthesized AgNPs showed good antibacterial activity against both gram-positive and gram-negative bacteria. The results suggest that R. communis leaf extract can be used effectively in the production of potential antioxidant and antibacterial AgNPs.

Antibacterial, Antioxidant, Phytochemicals, 
                Ricinus communis
              , Silver nanoparticles

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Author Information

Mintiwab Abrham
Department of Biology, College of Natural and Computational Science, Wolaita Sodo University, Wolaita Sodo, Ethiopia

Jeyaramraja P. R.
Department of Biology, College of Natural Sciences, Arba Minch University, Arba Minch, Ethiopia