Extracellular β-glucosidase produced by a wild edible mushroom Lentinus squarrosulus of Tripura

Debnath Gopal1,*, Das Sukla Rabi

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/2229-4473.2018.00068.X
First Page: 19
Last Page: 22
Views: 136


Keywords: Lentinus squarrosulus, β-glucosidase, temperature, pH, p-nitrophenyl-β-D-glucopiranoside.


Abstract


β-glucosidase is an enzyme that catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in β-D-glucosides and oligosaccharides with release of glucose. Microorganisms are potential sources of β-glucosidase enzyme production that can be employed for bioconversion of cellulose. In the present investigation, we focused on screening, production and characterization of extracellular β-glucosidase enzyme produced by wild edible mushroom Lentinus squarrosulus. The mycelial culture of L. squarrosulus producing brown colour of the medium (Cellulolysis basal medium supplemented with esculin) and possessed β-glucosidase activity positive. β-glucosidase enzyme production was maximum after 5th days incubation. Extracellular β-glucosidase enzyme activity was highest at 500C temperature, pH 5 and substrate concentration pnitrophenyl-β-D-glucopiranoside (p-NPG) at 1.4 mM. The present study revealed that wild edible mushroom L. squarrosulus has the ability to produce β-glucosidase enzyme and it may be utilized as a source for the production of industrial β-glucosidase for the bioconversion of cellulose to glucose.


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References



Acknowledgements



Author Information


Debnath Gopal1* Das Sukla Rabi

2Microbiology Laboratory Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India

*Corresponding author: Mycology and Plant pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India Email: gopaldn88@gmail.com