*Article not assigned to an issue yet
Keywords: Gerbera, Ornamental plant, ROS, Salinity, Thiourea
Salinity or salt stress is a key abiotic stress factor often correlated with ornamental plants grown in greenhouses, which are subjected to frequent application of fertilizers. Fertigation for prolonged periods in soil accumulates salts, creating salinity which interferes with the plant’s physiological function. Repeated fertigation results in a massive loss for commercially significant ornamentals like Gerbera jamesonii, whose cut blooms rank sixth in the global flower trade. In the current investigation, G. jamesonii plants (white and yellow flower kinds) were pre-treated with optimum amounts of Thiourea (1.0 mM, 5.0 mM, and 10.0 mM) to develop salt tolerance. Gerberas that were pre-treated with Thiourea and then exposed to NaCl (200 mM), have not shown any symptoms of plant stress or phenotypic abnormalities as compared to control plants that were not pre-treated with Thiourea. Furthermore, Thiourea application resulted in considerable increases in fresh and dry leaf weight, relative water content, total protein content, free proline content, and chlorophyll content. A decrease in H2O2 and MDA levels, as well as an increase in antioxidant enzyme activity such as superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, and peroxidase, indicate minimal cellular damage in salinity-exposed gerbera plants pre-treated with Thiourea. Thus, foliar spray of thiourea (1.0 mM) could help gerbera plants overcome the detrimental effects of salt stress, and the current findings may be extended to other ornamentals to promote sustainable development of the floriculture industry.
Aebi H (1974) Catalases. In: Bergmeyer Hu (ed) Methods of enzymatic analysis. Academic Press, New York. https://doi.org/10.1016/B978-0-12-091302-2.50032-3
Akladious SF (2014) Influence of thiourea application on some physiological and molecular criteria of sunflower (Helianthus annuus L.) plants under conditions of heat stress. Protoplasma 251:625–638. https://doi.org/10.1007/s00709-013-0563-2
Alexieva V, Sergiev I, Mapelli S, Karonov E (2001) The effect of drought and ultraviolet radiation on growth and stress marker in pea and wheat. Plant Cell Environ 24:1337–1344
Arif Y, Singh P, Siddiqui H, Bajguz A, Hayat S (2020) Salinity induced physiological and biochemical changes in plants: an omic approach towards salt stress tolerance. Plant Physiol Biochem 156:64–77. https://doi.org/10.1016/j.plaphy.2020.08.042
Baqer RA, Al-Kaaby HK, Adul-Qadir LH (2020) Antioxidant responses in wheat plants (Triticum aestivum L.) treated with Thiourea. Plant Arch 20(2):717–722
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207. https://doi.org/10.1007/BF00018060
Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161(2):559–566. https://doi.org/10.1016/0003-2697(87)90489-1
Cioc M, Dziurka M, Pawlowska B (2022) Changes in endogenous phytohormones of Gerbera jamesonii axillary shoots multiplied under different light emitting diodes light quality. Mol 27:1804. https://doi.org/10.3390/molecules27061804
Dhillon SA, Mujahid N, Shahbaz M, Debez A (2023) Response of sesame (Sesamum indicum L.) to foliar-applied thiourea under saline conditions. Intl J Appl Exp 2(2):167–177. https://doi.org/10.56612/ijaeb.v2i2.54
El-Dein SN, Hussein A, Abu-Bakr MS, El-Dein AN, Awad HM, Ragab EA (2023) Phytochemical analysis and determination of antioxidant, anti-cholesterol, anti-inflammatory and anti-proliferative activities of Gerbera jamesonii flowers. Adv Trad Med 23:863–875. https://doi.org/10.1007/s13596-022-00659-x
Elhindi KM, Almana FA, Al-Yafrsi MA (2023) Morpho-biochemical modification of petunia to saline water and salicylic acid applications. Horticulturae 9(11):1197
Es-sbihi FZ, Hazzoumi Z, Aasfar A (2021) Improving salinity tolerance in Salvia officinalis L. by foliar application of salicylic acid. Chem Biol Technol Agric 8:25. https://doi.org/10.1186/s40538-021-00221-y
Farooq M, Uzma J, Mamidala P (2024) Salicylic acid induced salt tolerance in Gerbera jamesonii, an ornamental plant. Vegetos. https://doi.org/10.1007/s42535-023-00794-z
Farzana S, Rasel M, Tahjib-Ul-Arif M, Al-Galib MA, Sarker KK, Hossain MA (2020) Exogenous salicylic acid and thiourea ameliorate salt stress in wheat by enhancing photosynthetic attributes and antioxidant defense. J Bangladesh Agric Univ 18(2):272–282. https://doi.org/10.5455/JBAU.86574
Foyer CH, Ruban AV, Noctor G (2017) Viewing oxidative stress through the lens of oxidative signalling rather than damage. Biochem J 474:877–883. https://doi.org/10.1042/BCJ20160814
Garg BK, Burman U, Kathju S (2006) Influence of thiourea on photosynthesis, nitrogen metabolism and yield of clusterbean (Cyamopsis tetragonoloba L.) Taub. under rainfed conditions of Indian arid zone. Plant Growth Regul 48:237–245. https://doi.org/10.1007/s10725-006-0002-x
Granaz SK, Baksh G, Zahra N, Hafeez MB, Raza A, Samad A, Nizar M, Wahid A (2022) Foliar application of thiourea, salicylic acid, and kinetin alleviate salinity stress in maize grown under etiolated and de-etiolated conditions. Discov Food 2:27. https://doi.org/10.1007/s44187-022-00027-3
Hafez M, Alexander I, Rashad M (2020) Evaluation of the effects of new environmental additives compared to mineral fertilizers on the leaching characteristics of some anions and cations under Greenhouse plant growth of saline-sodic soils. Open Agric 14:246–256. https://doi.org/10.2174/1874331502014010246
Hassanein RA, Amin ABAES, Rashad ESM, Ali H (2015) Effect of thiourea and salicylic acid on antioxidant defense of wheat plants under drought stress. Int J Chem Tech Res 7(1):346–354
Heath R, Packer L (1968) Photoperoxidation in isolated chloroplasts. Kinetics and stochiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Hernandez-Nistal J, Aldasoro J, Rodriguez D, Matilla A, Nicolas G (1983) Effect of thiourea on the ionic content and dark fixation of CO2 in embryonic axes of Cicer arietinum seeds. Physiol Plant 57:273–278. https://doi.org/10.1111/j.1399-3054.1983.tb00910.x
Hossain MS (2019) Present scenario of global salt affected soils, its management and importance of salinity research. Int Res J Biol Sci 1:1–3. https://doi.org/10.1007/978-3-030-78435-5_1
Kaya C, Sonmez O, Aydemir S, Ashraf M, Dikilitas M (2013) Exogenous application of mannitol and thiourea regulates plant growth and oxidative stress responses in salt-stressed maize (Zea mays L.). J Plant Interact 8(3):234–241. https://doi.org/10.1080/17429145.2012.725480
Kaya C, Ashraf M, Sönmez O (2015) Promotive effect of exogenously applied thiourea on key physiological parameters and oxidative defense mechanism in salt-stressed Zea mays L. Plants. Turk J Botany 39:786–795. https://doi.org/10.3906/bot-1409-10
Kaya C, Aydemir S, Polat T, Ashraf M, Tuna L, Sonmez O (2016) Exogenous application of nitric oxide and thiourea regulates on growth and some key physiological processes in maize (Zea mays L.) plants under saline stress. Toprak S Dergisi. https://doi.org/10.21657/tsd.36165
Lichtenthaler K, Welburn AR (1983) Determination of total carotenoids and chlorophylls A and B of leaf extracts in different solvents. Biochem Soc Trans 11:591–592. https://doi.org/10.1042/bst0110591
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265
Mbarki S, Sytar O, Cerda A, Zivcak M, Rastogi A, He X (2018) Strategies to mitigate the salt stress effects on photosynthetic apparatus and productivity of crop plants. In: Kumar V, Wani SH, Suprasanna P, Tran L-SP (eds) Salinity responses and tolerance in plants. Springer, Cham. https://doi.org/10.1007/978-3-319-75671-4_4
Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol 22:867–880. https://doi.org/10.1093/oxfordjournals.pcp.a076232
Pandey M, Srivastava AK, D’Souza SF, Penna S (2013) Thiourea, a ROS scavenger, regulates source-to-sink relationship to enhance crop yield and oil content in Brassica juncea (L.). PLoS ONE 8:e73921. https://doi.org/10.1371/journal.pone.0073921(2013)
Perveen T, Nawaz K (2021) Modulation of morpho-physiological and biochemical attributes of Solanum melongena L. (brinjal) by exogenous application of thiourea under salinity stress. Pak J Bot 53(6):1969–1978. https://doi.org/10.30848/PJB2021-6(19)
Perveen S, Farooq R, Shahbaz M (2016) Thiourea-induced metabolic changes in two mung bean [Vigna radiata (L.) Wilczek] (Fabaceae) varieties under salt stress. Brazilian J Bot 39:41–54. https://doi.org/10.1007/s40415-015-0209-z
Reinten E, Coetzee J, Van Wyk BE (2011) The potential of South African indigenous plants for the international cut flower trade. S Afr J Bot 77:934–946. https://doi.org/10.1016/j.sajb.2011.09.005
Sa G, Yao J, Deng C, Liu J, Zhang Y, Zhu Z (2019) Amelioration of nitrate uptake under salt stress by ectomycorrhiza with and without a hartig net. New Phytol 222:1951–1964. https://doi.org/10.1111/nph.15740
Sarker U, Oba S (2018) Drought stress enhances nutritional and bioactive compounds, phenolic acids and antioxidant capacity of amaranthus leafy vegetable. BMC Plant Biol 18:258. https://doi.org/10.1186/s12870-018-1484-1
Seleiman MF, Kheir AMS (2018) Saline soil properties, quality and productivity of wheat grown with bagasse ash and thiourea in different climatic zones. Chemosphere 193:538–546. https://doi.org/10.1016/j.chemosphere.2017.11.053
Shabala S, Wu H, Bose J (2015) Salt stress sensing and early signaling events in plant roots: current knowledge and hypothesis. Plant Sci 241:109–119. https://doi.org/10.1016/j.plantsci.2015.10.003
Shannon LM, Kay E, Lew JL (1966) Peroxidase isozymes from horse radish roots: isolation and physical properties chemistry and metabolism of macromolecules. J Biol Chem 241(9):2166–2172. https://doi.org/10.1016/S0021-9258(18)96680-9
Sharma DK, Chaudhari SK, Singh A (2014) In salt-affected soils agroforestry is a promising option. Indian Farming 63(11):19–22
Singh G (2009) Salinity-related desertification and management strategies: Indian experience. Land Degrad Dev 20(4):367–385. https://doi.org/10.1002/ldr.933
Singh P, Bhardwaj A, Kumar R, Singh D (2017) Evaluation of Gerbera varieties for yield and quality under protected environment conditions in Bihar. Int J Curr Microbial App Sci 6(9):112–116. https://doi.org/10.20546/ijcmas.2017.609.013
Srivastava AK, Ramaswamy NK, Mukopadhyaya R, Jincy MGC, D’souza SF (2009) Thiourea modulates the expression and activity profile of mtATPase under salinity stress in seeds of Brassica juncea. Ann Bot 103:403–410. https://doi.org/10.1093/aob/mcn229
Srivastava K, Srivastava S, Penna S, D’Souza SF (2011) Thiourea orchestrates regulation of redox state and antioxidant responses to reduce the NaCl-induced oxidative damage in Indian mustard (Brassica juncea (L.). Plant Physiol Biochem 49(6):676–686. https://doi.org/10.1016/j.plaphy.2011.02.016
Srivastava AK, Sablok G, Hackenberg M, Deshpande U, Suprasanna P (2017) Thiourea priming enhances salt tolerance through co-ordinated regulation of microRNAs and hormones in Brassica juncea. Sci Rep 7:45490. https://doi.org/10.1038/srep45490
Srivastava AK, Srivastava S, Penna S, D’Souza SF (2019) Thiourea orchestrates regulation of redox state and antioxidant responses to reduce the NaCl-induced oxidative damage in Indian mustard (Brassica juncea L.) Czern.). Plant Physiol Biochem 49:676–686. https://doi.org/10.1016/j.plaphy.2011.02.016
Suryavanshi P, Buttar GS (2018) Effects of exogenous osmoprotectants on physiological characteristics of wheat. Int J Curr Microbiol App Sci 7:1077–1089
Talukdar D (2017) Exogenous thiourea modulates antioxidant defence and glyoxalase systems in lentil genotypes under arsenic stress. J Plant Stress Physiol 2:29. https://doi.org/10.19071/jpsp.2016.v2.3041
Uzma J, Talla SK, Madam E, Mamidala P (2022a) Assessment of salinity tolerance deploying antioxidant defense systems in Gerbera Jamesonii. Biosci Biotech Res Asia 19(1):243–254. https://doi.org/10.13005/bbra/2982
Uzma J, Talla SK, Madam E, Mamidala P (2022b) Salinity-driven oxidative stress in Gerbera jamesonii cv Bolus. J Appl Hortic 24(2):240–244. https://doi.org/10.37855/jah.2022.v24i02.44
Uzma J, Talla SK, Mamidala P (2023) Insights into the impact of spermidine in reducing salinity stress in Gerbera jamesonii. J Appl Boil 11(4):141–147. https://doi.org/10.7324/JABB.2023.120550
Vineeth TV, Kumar P, Krishna GK (2016) Bioregulators protected photosynthetic machinery by inducing expression of photorespiratory genes under water stress in chickpea. Photosynthetica 54:234–242. https://doi.org/10.1007/s11099-016-0073-5
Waqas MA, Kaya C, Riaz A, Farooq M, Nawaz I (2019) Potential mechanisms of abiotic stress tolerance in crop plants induced by thiourea. Front Plant Sci 10:1336. https://doi.org/10.3389/fpls.2019.01336
Yang Y, Guo Y (2018) Unraveling salt stress signaling in plants. J Integrat Plant Biol 60:796–804. https://doi.org/10.1111/jipb.12689
Department of Biotechnology, Telangana University, Nizamabad, India