Fungal endophytes from medicinal plants: growth promotion in Oryza sativa L. and Cicer arietinum L.

, , ,

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00043-2
First Page: 381
Last Page: 386
Views: 1794


Keywords: Growth promoting endophytic fungi, Internal transcribed spacer, Cicer arietinum , Oryza sativa


Abstract

In the present investigation, four endophytic fungi were isolated from two medicinal plants from two medicinal plants and identified using molecular technique and tested the efficacy of four isolates on the growth of Cicer arietinum and two varieties of rice (Sarath and Gomati). The molecular characterization using amplification of internal transcribed spacer (ITS) region of fungal ribosomal DNA (rDNA) reveals four fungi namely Diaporthe phaseolorum, Penicillium oxalicum, Fusarium sp. and Syncephalastrum monosporum. The efficacy of isolates on the growth of plants indicated that there was significant increase in shoot length, root length and shoot dry weight with inoculation of D. phaseolorum, P. oxalicum and Fusarium sp. on Sarath variety of rice. Significant increase in shoot length and root dry weight was observed in Gomati rice variety with the inoculation of D. phaseolorum, P. oxalicum and Fusarium sp. Again there was significant increase in root length, shoot dry weight and root dry weight with inoculation of D. phaseolorum, P. oxalicum and Fusarium sp. on the growth of C. arietinum. However, no significant difference was observed between S. monosporum and uninoculated treatment on the growth of rice variety Gomati and C. arietinum. The results indicated that out of four, P. oxalicum, Fusarium sp. and D. phaseolorum were better plant growth promoting endophytic fungi.


Growth promoting endophytic fungi, Internal transcribed spacer, Cicer arietinum , Oryza sativa


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References

  1. Aljanabi SM, Martinez I (1997) Universal and rapid salt extraction of high quality genomic DNA for PCR based techniques. Nucleic Acids Res 25:4692–4693

  2. Aly AH, Debbab A, Kjer J, Proksch P (2010) Fungal endophytes from higher plants: a prolific source of phytochemicals and other bioactive natural products. Fungal Divers 41:1–16

  3. Azevedo JL, Maccheroni JW, Pereira JO, Araújo WL (2000) Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electron J Biotechnol 3:40–65

  4. Bagchi B, Banerjee D (2013) Diversity of fungal endophytes in Bauhinia vahlii (a lianas) from different regions of Paschim Medinipur district of West Bengal. Int Sci Environ Technol 2:748–756

  5. Bezerra JDP, Nascimento CCF, Barbosa RDN, Silva DCV, Svedese VM, Nogueira Silva EB, Gomes BS, Paiva LM, Souza-Motta CM (2015) Endophytic fungi from medicinal plant Bauhinia forficata: diversity and biotechnological potential. Braz J Microbiol 46(1):49–57

  6. Bitas V, Mc CN, Li N, DemersJ Kim JE, Kim HS, Brown KM, Kang S (2015) Fusarium oxysporum volatiles enhance plant growth via affecting auxin transport and signaling. Front Microbiol 6:1248

  7. Brown KB, Hyde KD, Guest DI (1998) Preliminary studies on endophytic fungal communities of Musa acuminata species complex in Hong Kong and Australia. Fungal Divers 1:27–51

  8. Crous PW, Braun U, Schubert K, Groenewald JZ (2007) Delimiting Cladosporium from morphologically similar genera. Stud Mycol 58:33–56

  9. Hardoim PR, Van OLS, Berg G, Pirttilä AM, Compant S, Campisano A, Döring M, Sessitsch A (2015) The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. Microbiol Mol Biol Rev 79(3):293–320

  10. Horton TR, Bruns T (2001) The molecular revolution in ectomycorrhizal ecology: peeking into the black-box. Mol Ecol 10:1855–1871

  11. Jarvis WR, Barrie SD, Traquair JA, Stoessl A (1990) Morphological and chemical studies of Penicillium oxalicum, newly identified as a pathogen on greenhouse cucumbers. Can J Bot 68(1):21–25

  12. Kauserud H, Lie M, Stensrud Ø, Ohlson M (2005) Molecular characterization of airborne fungal spores in boreal forests of contrasting human disturbance. Mycologia 97(6):1215–1224

  13. Machungo C, Losenge T, Kahangi E, Coyne D, Dubois T, Kimenju JW (2009) Effect of endophytic Fusarium oxysporum on growth of tissue-cultured banana plants. Afric J Hortic Sci 2:160–167

  14. Morakotkarn D, Kawasaki H, Seki T (2007) Molecular diversity of bamboo-associated fungi isolated from Japan. FEMS Microbiol Lett 266:10–19

  15. Pennisi E (2004) The secret life of fungi. Science 304:1620–1622

  16. Petrini O (1991) Fungal endophytes of tree leaves. In: Andrews JH, Hirano SS (eds) Microbiol ecol leaves. Springer-Verlag, New York, pp 179–197

  17. Pioli RN, Morandi EN, Martínez MC, Lucca F, Tozzini A, Bisaro V, Hopp HE (2003) Morphologic, molecular, and pathogenic characterization of Diaporthe phaseolorum variability in the core soybean-producing area of Argentina. Phytopathol 93(2):136–146

  18. Saikkonen K, Helander M, Faeth SH (2004) Fungal endophytes: hitchhikers of the green world. In: Gillings M, Holmes A (eds) Plant microbiol. BIOS Scientific Publishers, Oxford, pp 77–95

  19. Sette LD, Passarini MRZ, Delarmelina C, Salati F, Duarte MCT (2006) Molecular characterization and antimicrobial activity of endophytic fungi from coffee plants. World J Microbiol Biotechnol 22:1185–1195

  20. Shankar NB, Krishnamurthy YL (2010) Endophytes: the real untapped high energy biofuel resource. Curr Sci 98:883

  21. Sharma R, Singh SK (2013) A new species of Gymnoascus with verruculose ascospores. IMA Fungus 4(2):177–186

  22. Sieber TN (2002) Fungal root endophytes. In: Waisel Y, Eshel A, Kafkafi U (eds) The hidden half. Dekker, New York, pp 887–917

  23. Sun JQ, Guo LD, Zang W, Ping WX, Chi DF (2008) Diversity and ecological distribution of endophytic fungi associated with medicinal plants. Sci In China Ser C Life Sci 51:751–759

  24. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739

  25. Tan RX, Zou WX (2001) Endophytes: a rich source of functional metabolites. Natl Prod Rep 18(448):459

  26. Tan RX, Meng JC, Hostettmann K (2000) Phytochemical investigation of some traditional Chinese medicines and endophyte cultures. Pharm Biol 38:22–32

  27. Waqas M, Khan AL, Kamran M, Hamayun M, Kang SM, Kim YH, Lee IJ (2012) Endophytic fungi produce gibberellins and indoleacetic acid and promotes host-plant growth during stress. Molecules 17:10754–10773

  28. White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, California, pp 315–322


    29.  


Acknowledgements

The first author is grateful to UGC, Govt. of India for BSR fellowship. The authors are thankful to Head, Department of Botany, Tripura University for providing laboratory facilities. The authors also acknowledge Agharkar Research Institute, Pune, India for help in molecular identification of fungi.


Author Information

Bhattacharjee Sukla
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India

Roy Das Aparajita
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India


Saha Ajay Krishna
Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Agartala, India


Das Panna
Microbiology Laboratory, Department of Botany, Tripura University, Agartala, India

panna11d@gmail.com