Genetic variation in sugarcane cultivars for red rot resistance revealed by resistant gene analog polymorphism markers

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00086-5
First Page: 92
Last Page: 99
Views: 1995


Keywords: Saccharum , RGAP, Genetic diversity, Colletotrichum falcatum , Red rot, Resistance


Abstract

Genetic diversity among 55 different sugarcane (Saccharum) cultivars varying in their response for red rot disease (Colletotrichum falcatum) was studied using Resistance Gene Analog Polymorphism (RGAP) markers. A total of 28 primers were used in the present study to screen 30 moderately resistant, five resistant, seven moderately susceptible, nine susceptible and four highly susceptible cultivars resulted in the generation of 87 distinct fragments with 91.9% polymorphism. Polymorphism Information Content (PIC) and Resolving Power (RP) were also calculated and the values were found to be 16.28 and 52.36 respectively. On analyzing the dendrogram, a clear distinctive grouping was observed between red rot resistant and red rot susceptible cultivars. This study, therefore clearly revealed that Resistant Gene Analog (RGA) markers showed greater genetic diversity and hence, constitute a rich source for analyzing the genetic diversity and phylogenetic relationships. The genotypes belonging to different clusters can be crossbreed to gain high variability of good combinations of characters.


Saccharum , RGAP, Genetic diversity, Colletotrichum falcatum , Red rot, Resistance


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Acknowledgements

The authors are thankful to the Head, Department of Biotechnology, Kumaun University, Nainital, India for providing lab facilities.


Author Information

Sharma Ruchika
Plant Tissue Culture and Molecular Biology Laboratory, Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital, India
rsharma.88@outlook.com