Green biosynthesis of silver nanoparticles and impact on growth, chlorophyll, yield and phytotoxicity of Phaseolus vulgaris L

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-020-00150-5
First Page: 648
Last Page: 657
Views: 1964

Keywords: Chlorophyll, Silver nanoparticles, Phaseolus vulgaris , Phytotoxicity


Silver nanoparticles (Ag NPs) are used worldwide for research experiments in various fields. The green biosynthesis method used for the synthesis of Ag NPs and its characterization was completed by PSA (particle size analyzer), SEM (scanning electron microscopy), XRD (X-ray diffraction) and TEM (transmission electron microscopy). The objective of this research study was to find out the effect of biosynthesis Ag NPs on seed germination, root elongation, shoot growth, photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids), plant improvement and yield production, etc. of Phaseolus vulgaris L. Six different concentrations such as 0, 15, 30, 60, 120, 240 and 480 mg L−1 were applied after seed sterilization by seed treatment method. Morphological parameters and phytochemicals of plants treated at different concentrations were compared with control plants. Chlorophyll increase in phytochemical activity was observed in seeds treated with low concentrations of Ag nanoparticles. While higher concentrations showed greater toxicity, but root elongation was observed in seeds treated with 120 mg L−1 concentration.

Chlorophyll, Silver nanoparticles, 
                Phaseolus vulgaris
              , Phytotoxicity

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The authors are thankful to the School of Studies in Botany, Central Instrumentation Facility (CIF) for providing available facilities and the financial support by Jiwaji University for this research work and also thanks to the Tezpur University, Assam for SEM analysis of Ag NPs.

Author Information

Verma Deepak Kumar
School of Studies in Botany, Jiwaji University, Gwalior, India

Patel Sapan
School of Studies in Botany, Jiwaji University, Gwalior, India

Kushwah Kalyan Singh
School of Studies in Botany, Jiwaji University, Gwalior, India