Keywords: Chlorophyll, Silver nanoparticles, Phaseolus vulgaris , Phytotoxicity
Silver nanoparticles (Ag NPs) are used worldwide for research experiments in various fields. The green biosynthesis method used for the synthesis of Ag NPs and its characterization was completed by PSA (particle size analyzer), SEM (scanning electron microscopy), XRD (X-ray diffraction) and TEM (transmission electron microscopy). The objective of this research study was to find out the effect of biosynthesis Ag NPs on seed germination, root elongation, shoot growth, photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids), plant improvement and yield production, etc. of Phaseolus vulgaris L. Six different concentrations such as 0, 15, 30, 60, 120, 240 and 480 mg L−1 were applied after seed sterilization by seed treatment method. Morphological parameters and phytochemicals of plants treated at different concentrations were compared with control plants. Chlorophyll increase in phytochemical activity was observed in seeds treated with low concentrations of Ag nanoparticles. While higher concentrations showed greater toxicity, but root elongation was observed in seeds treated with 120 mg L−1 concentration.
The authors are thankful to the School of Studies in Botany, Central Instrumentation Facility (CIF) for providing available facilities and the financial support by Jiwaji University for this research work and also thanks to the Tezpur University, Assam for SEM analysis of Ag NPs.