Hypoglycemic, antioxidant, antimicrobial activities and phytochemical analyses of Rhaponticoides iconiensis leafy stem extracts

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00250-w
First Page: 592
Last Page: 599
Views: 625

Keywords: Diabet, α-Glucosidase, α-Amylase, Antimicrobial activity, LC–MS/MS


Rhaponticoides iconiensis (Hub.-Mor.) M.V.Agab. & Greuter is one of the species of Asteraceae family. In this study, the α-amylase and α-glucosidase inhibition activity, the antioxidant, antimicrobial properties and the phytochemical composition of extract and sub-extracts from the leafy stem of R. iconiensis were evaluated. The antimicrobial effect of extracts was tested with the microdilution method and the antioxidant activity was investigated with DPPH·, ABTS·+ and FRAP tests. The phytochemical composition of active sub-extract carried out by LC–MS/MS. As a result, the highest α-glucosidase and α-amylase activity were found in ethyl acetate sub-extract (TSE) of R. iconiensis leafy stem extract (IC50 = 12.108 ± 0.582 µg/mL and 287.733 ± 0.325 µg/mL, respectively). Moreover, the highest total phenolic (312.001 ± 2.008 mgGAE/gextract) and total flavonoid content (242.007 ± 2.646 mgCA/gextract) was found in TSE extract too and this extract was found more active on DPPH· (IC50 = 0.249 ± 0.002 mg/mL), ABTS+· (at a concentration of 0.5 mg/mL equivalent to 0.542 ± 0.002 µM Trolox) and FRAP (at a concentration of 1 mg/mL equivalent to 3642.005 ± 2.646 mmol Fe2+) tests. Besides that, the flavonoids such as apigenin, naringin and patuletin derivatives were determined as the main compounds of active TSE sub-extract. This is the first report that these biological activities investigated and phytochemical compounds were identified in leafy stem extracts of R. iconiensis and it is thought that these studies will be shed light on future studies on this species.

                        α-Amylase, Antimicrobial activity, LC–MS/MS

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The authors thank to Erciyes University Drug Application and Research Center (ERFARMA) for their support in the use of LC-MS/MS in this study.

Author Information

Paşayeva Leyla
Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey
İnce Ufuk
Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey

Fatullayev Hanifa
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Erciyes University, Kayseri, Turkey

Ceylan Cengizhan
Department of Clinical Pharmacy, Faculty of Pharmacy, Selçuk University, Konya, Turkey

Tugay Osman
Department of Pharmaceutical Botany, Faculty of Pharmacy, Selçuk University, Konya, Turkey