Identification and characterization of a fertility restorer line in Bhut Jolokia

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00566-1
First Page: 1517
Last Page: 1525
Views: 1314


Keywords: Cytoplasmic male sterility, Fertility restorer, Capsaicin, Pungency


Abstract


Cytoplasmic male sterility is an important trait in hybrid seed production. Restorer lines are required for production of F1 hybrid seed using cytoplasmic male sterile line. Restorer lines have been identified and characterized only in hot chili varieties of Capsicum annuum. In this study, we screened Bhut Jolokia genotypes for their ability to restore male fertility of a cytoplasmic male sterile line, CCA 4261. Data on fruit set, visual pollen score, pollen viability and anther sections in F1 hybrids of CCA 4261 and Bhut Jolokia showed that a functional restorer of fertility (Rf) gene was present in Bhut Jolokia genotype CC 215 but absent in other genotypes. The inheritance of this Rf gene conformed well with the incomplete dominance model for a single gene. However, the Rf linked marker, CRF-SCAR, known to be linked to Rf locus in C. annuum did not produce any amplicon in CC 215. Identification of restorer in Bhut Jolokia will help in developing high yielding F1 hybrids in this segment of super-hot peppers and increase farm income.


Cytoplasmic male sterility, Fertility restorer, Capsaicin, Pungency


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Acknowledgements


The authors acknowledge TERI School of Advanced studies for their regular support and specifically Department of Biotechnology, Government of India, for their financial support.


Author Information


Singh Vivek Kumar
TERI School of Advanced Studies, New Delhi, India

Malik Anoop Anand
The Energy and Resources Institute, North-Eastern Regional Centre, Guwahati, India


Islam Md Aminul
Majuli College, Majuli, India


Sharma Shyam Sundar
The Energy and Resources Institute, New Delhi, India


Tripathi Shashi Bhushan
TERI School of Advanced Studies, New Delhi, India
shashi.tripathi@terisas.ac.in