Vegetos
(An International Journal of Plant Research & Biotechnology)
In vitro culture of Consolea millspaughii subsp. millspaughii (Cactaceae): a critically endangered species
Martel-Ortiz Dairo, González-De Zayas Roberto, Concepción Oscar, Escalona Maritza, Hajari Elliosha, Lorenzo José Carlos, Hernández-Fernández Leslie
Short Communications | Published: 08 July, 2024
First Page: 1707
Last Page: 1712
Views: 1817
Keywords: Cactus, Climate change, Diversity conservation, Island ecosystems, Soil salinization
Abstract
Islands are biodiversity hotspots yet accelerated levels of land degradation as a consequence of infrastructure development is posing a serious threat to native species. Cuba contains the largest diversity of cacti in the Caribbean, however, some are critically endangered such as those within the genus Consolea. Therefore, the present study undertook a census of Consolea millspaughii subsp. millspaughii on the Cayo Paredón Grande in Cuba to assess the current status of populations. A secondary goal of the study was to develop the preliminary stages for a micropropagation protocol for this species. The results showed a worrying decline in adult cacti from the last census conducted in 2019 (a reduction from 15 to 5 adult plants). However, up to 41 juvenile plants were noted which is encouraging from a conservation perspective. The early stages of the micropropagation protocol were established with NaClO being sufficient to remove surface decontaminants. Up to 100% sprouting was obtained on medium supplemented with 4.43 µmol L−1 benzyladenine and 66.7% rooting was obtained on medium devoid of plant growth regulators. Future studies will involve refinement of the micropropagation protocol so that plants can be multiplied for restoration efforts to support conservation of this endangered species.
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Author Information
Bioplant Center, University of Ciego de Ávila, Ciego de Ávila, Cuba