In vitro propagation of insectivorous plant Nepenthes khasiana Hook. F.- an endangered ornamental and ethnomedicinal species

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00310-1
First Page: 534
Last Page: 539
Views: 407


Keywords: n Nepenthes khasianan , Carnivorous, Endemic, Critically endangered, Shoot tip, Micropropagation


Abstract


Nepenthes khasiana Hook. f. is a critically endangered, carnivorous, endemic species of the Assam and Meghalaya and belongs to the monotypic family Nepenthaceae. An efficient micropropagation protocol was optimized for the propagation and ex-situ conservation of elite germplasm of the species from axenic shoot tip culture. The axenic cultures were established through in vitro seed germination, which envisage the prolonged seed germination time (180 Days) and poor seed germination (25%) in the species. Application of GA3 (500 ppm) enhanced the seed germination upto 89%. Half-strength Murashige and Skoog (MS) medium fortified with 4.4 µM BAP was reported to be the ideal medium and 96.81% shoot formation was observed in N. khasiana shoot tip explants. The highest shoot proliferation of 21.22 ± 0.78 shoots per explants with 10.5 ± 0.5 cm mean shoot length was recorded. The in vitro raised shoots rooted in half salt strength MS medium fortified with IBA (4.9 μM) and 92.11% rooting was recorded with average 17.58 ± 0.87 roots per shoot and 5.5 ± 0.29 cm root length. In-vitro raised plantlets were shifted to the glass house with 65% success rate. Thus, the protocol developed might be useful for the ex-situ conservation of germplasm and commercialization of species to cope up with the ever increasing medicinal and ornamental demands of the species.

n              Nepenthes khasianan            , Carnivorous, Endemic, Critically endangered, Shoot tip, Micropropagation


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References


Bahadur V, Kirad KS, Mathew A, Singh DB (2008) Tissue culture studies in Nepenthes khasiana. Acta Hortic 786:287–293


Betlu ALS (2016) Nepenthes khasiana Hook. F. (Nepenthaceae) from Assam. Curr Sci 111(8):1311


Bordoloi RPM (1977) The pitcher plant Nepenthes khasiana. Sreeguru Press, Guwahati


Devi SP, Kumaria S, Rao SR, Tandon P (2013) In vitro propagation and assessment of clonal fidelity of Nepenthes khasiana Hook. f.: a medicinal insectivorous plant of India. Acta Physiol Plant. https://doi.org/10.1007/s11738-013-1314-x


Devi SP, Kumaria S, Sharma PR, Khoyumthem P, Tandon P (2019) Nepenthes khasiana Hook. F., an endangered tropical pitcher plant of India. Indian J Tradition Knowl 18(1):68–75


Khuraijam JS, Roy RK (2015) Propagation of threatened Nepenthes khasiana: methods and precautions. Not Sci Biol 7(3):313–315


Kitching R, Schofield C (1986) Every pitcher tells a story. New Sci 23:48–51


Latha SS, Seeni S (1994) Multiplication of the endangered Indian pitcher plant (Nepenthes khasiana) through enhanced axillary branching in vitro. Plant Cell Tiss Org Cult 38:69–71


Mao AA, Kharbuli P (2002) Distribution and status of Nepenthes khasiana Hook. f., a rare endemic pitcher plant of Meghalaya, India. Phytotaxonomy 2:77–83


Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497


Nongrum L, Kumaria S, Tandon T (2009) Multiplication through in vitro seed germination and pitcher development in Nepenthes khasiana Hook. F., a unique insectivorous plant of India. J Horticult Sci Biotechnol 84(3):329–332


Prasad MNV, Jeeva S (2009) Coal mining and its leachate are potential threats to Nepenthes khasiana Hook. F. (Nepenthaceae) that preys on insects-an endemic plant in North Eastern India. Bio Di Con 2/3:29–33


Rathore TS, Tandon P, Shekhawat NS (1991) In vitro regeneration of the pitcher plant (Nepenthes khasiana Hook. f.)-A rare insectivorous plant of India. J Plant Physiol 139:246–248


Redwood GN, Bowling JC (1990) Micropropagation of Nepenthes sp. Royal Botanic Gardens. Micropropagation News 1:19–20


Tandon P, Rathore TS (1994) Mass clonal propagation of the threatened Indian insectivorous plant (Nepenthes khasiana) through shoot bud culture. In: Tandon P (ed) Advances in plant tissue culture in India. Pragati Prakashan, New Delhi, pp 185–192


Tandon P, Kumaria S (1998) Threats to plant diversity in high altitude of North-East India and conservation of rare and endangered plants using biotechnological approaches. In: Saha SP, Ray K, Sinha B (eds) Science at high altitude. Allied Publishers Ltd, India, pp 140–147


Ziemer B (2010) Exciting conservation news: the rare Nepenthes collection project! Carniv Plant Newsl 39(3):67


Devi R, Venugopal N (2006) The status of insectivorous plants in North-East India: their uses and conservation. JSwamy Bot Club 23(1–4): 75–80

 


Acknowledgements


Authors are thankful to Director, Botanical Survey of India, Kolkata for the infrastructure and financial support.


Author Information


Joshi Bhavana
Botanical Survey of India, Northern Regional Centre, Dehradun, India

Singh S. K.
Botanical Survey of India, Northern Regional Centre, Dehradun, India