In vitro root proliferation from different explants of Solanum nigrum L.


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00512-1
First Page: 1384
Last Page: 1390
Views: 1386


Keywords: n Solanum nigrum L.n , MS media, In vitro propagation, NAA, IBA, 2, 4-D, IAA, BAP


Abstract


Solanum nigrum L. is a common edible medicinal herb of Solanaceae family, native to South East Asia, widely distributed to temperate and tropical regions of Europe, Asia and America. Traditionally, it has been valued to treat various types of cancers and acute ulcers due to presence of steroidal glycoalkaloid, phenols and polysaccharides as major bioactive constituents. Currently, plant based pharmaceutical companies are dependent on natural habitat for procurement of these bioactive compounds which is not sustainable for biodiversity conservation. Therefore, in vitro propagation is one of the important strategies for biodiversity conservation which has a significant impact on plant breeding, horticulture and medicine development as well as for the availability of the plant throughout the year. During present investigation, an efficient in vitro root proliferation was observed from in vivo grown stem and leaf explants on MS media. This paper deals with effects of various plant growth regulators on root organogenesis from mature leaves and stem explants obtained from in vivo grown Solanum nigrum L. plant. Various plant growth regulators like NAA, IBA, IAA, 2, 4-D, BAP were supplemented individually and in different combination and concentration. This article presents a comprehensive, systematic overview and statistical data on root formation from nodal and internodal stem and leaf explants. The present work deals with roots and hairy root formation when MS media was fortified with low concentration (0.05, 0.5 mg/l) of auxins. This protocol can be used for large scale production of roots and root hairs for accumulation of secondary metabolites in suspension culture.


n                     Solanum nigrum L.n                  , MS media, In vitro propagation, NAA, IBA, 2, 4-D, IAA, BAP


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Acknowledgements



Author Information


Kumari Priyanka
Research Scholar, University Department of Botany, Ranchi University, Ranchi, India
satyasupriya17551@gmail.com