Isolation and Characterization of Osmotolerant Rhizobium sp. from Vigna mungo (L.) Hepper Grown in Semi-arid Region

Saraf Renuka, Chaudhary Shweta, Chakraborty Dipjyoti*


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/2229-4473.2018.00039.3
First Page: 102
Last Page: 107
Views: 118


Keywords: Legume plant, <I>R hiz obium</I>, <I>Vigna mungo</I>, osmotolarent bacteria.


Abstract


Bacteria were isolated from the root nodule of the Vigna mungo, grown in a semi-arid region. The isolates were cultured at different concentration of NaCl (0–5%) YEMA supplemented medium. Out of several isolates that were able to tolerate high salt concentration, the colony characteristics of only one isolate was identical to Rhiz obium. Several biochemical assays were done for characterization. Molecular characterization by 16 SrRNA confirmed its identification. The aim of the isolation of rhizobial bacteria associated with V. mungo included the assessment of osmotolerant R hiz obium sp. so as to improve the productivity in susceptible plants on cross inoculation.


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References



Acknowledgements



Author Information


Saraf Renuka Chaudhary Shweta Chakraborty Dipjyoti*
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India

*Corresponding author: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India E-mail: cdipjyoti@banasthali.in