Saraf Renuka, Chaudhary Shweta, Chakraborty Dipjyoti*
Keywords:
Legume plant, <I>R hiz obium</I>, <I>Vigna mungo</I>, osmotolarent bacteria.
Bacteria were isolated from the root nodule of the Vigna mungo, grown in a semi-arid region. The isolates were cultured at different concentration of NaCl (0–5%) YEMA supplemented medium. Out of several isolates that were able to tolerate high salt concentration, the colony characteristics of only one isolate was identical to Rhiz obium. Several biochemical assays were done for characterization. Molecular characterization by 16 SrRNA confirmed its identification. The aim of the isolation of rhizobial bacteria associated with V. mungo included the assessment of osmotolerant R hiz obium sp. so as to improve the productivity in susceptible plants on cross inoculation.
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*Corresponding author: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, India E-mail: cdipjyoti@banasthali.in