Isolation, screening, and optimization of culture parameters for enhanced lipase production from Aspergillus eucalypticola strain CBS 122,712 and its application

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-022-00431-1
First Page: 70
Last Page: 78
Views: 182

Keywords: Screening, Optimization, A. eucalypticola , Lipase, Production


Lipase has high commercial value and attributes to various application ranges. A lipolytic fungus producing the extracellular lipase was isolated from a soil sample collected from the mustard oil processing industry. Based on 18 S rRNA sequencing, it was identified as Aspergillus eucalypticola strain CBS 122,712. In this report, the isolation, screening, identification, optimization of culture parameters, and oil stain removal application are reported. Various culture conditions were optimized and the maximum lipase activity was reported with ammonium nitrate (126.92 ± 0.03 IU/ml/min) at 25℃ after 5 days of incubation. As per our knowledge, this is the first report on optimization of culture parameters for lipase production by A. eucalypticola and crude protein lysate of A. eucalypticola in combination with detergent which can be used for lipidic stain removal and for laundry purposes.

Screening, Optimization, 
                A. eucalypticola
              , Lipase, Production

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We would like to thank Vice-Chancellor, Banasthali Vidyapith for providing admirable research facilities.

Author Information

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, India

Bhati Nikita
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, India

Sharma Arun Kumar
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, India