Micropropagation of an ethnomedicinal plant Solanum torvum Swartz


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00502-3
First Page: 1305
Last Page: 1312
Views: 1384


Keywords: Plant growth regulators, Micropropagation, Shoot tip explants, Nodal explants, n In vitro rooting, Plantlet establishment


Abstract


In the current study, we described the impact of various plant growth regulators (PGRs) on the micropropagation of S. torvum (Turkey Berry), a crucial species for ethanomedicine. The explants were cultured on MS medium without PGRs (MSO) as well as MS medium that was fortified with 0.1/0.5 mg/L IAA and various concentrations of BAP/KIN alone. In comparison to KIN alone and IAA + KIN combination, BAP alone and in combination with IAA elicited more numerous shoots in both of the explants of S. torvum that were examined. At 0.1/0.5 mg/L IAA + 1.0–5.0 mg/L BAP/KIN, both explants demonstrated the highest response percentage (80–96%). As opposed to all other concentration combinations of PGRs employed, the highest frequency number of shoots (42.0 ± 0.23) with long (15.2 ± 0.13 cm) shoots were discovered at 0.5 mg/L IAA + 4.0 BAP from shoot tip explants. Thus, it is evident that in the current experiments, shoot tip explants were discovered to have a greater potential for producing a high frequency number of shoots per explant than nodal explants. The elongated micro-shoots produced in both cultures were transferred to root induction medium (RIM), which was supplemented with auxins at concentrations ranging from 0.25 to 2.0 mg/L NAA, IBA, and IAA, for in vitro rooting. At 1.0 mg/L IAA, the highest response rate (90%), the most roots per micro-shoot (17.6 ± 0.15), and the longest roots (8.5 ± 0.16) were seen. In vitro rooted plantlets were placed in sterile vermiculite and soil (1:1) mixture before being adjusted to the greenhouse environment. Plantlets that had been in vitro rooted were transplanted to the field. 90% of the plantlets survived, and their morphology, flowering, and fruiting were all deemed normal. The micropropagation procedure created in the current study can therefore be employed for genetic transformation research to introduce novel genes in the ethnomedicinal plant S. torvum as well as for conservation.


Plant growth regulators, Micropropagation, Shoot tip explants, Nodal explants, n                     In vitro rooting, Plantlet establishment


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Acknowledgements


We thank the University Grants Commission, New Delhi, India for providing the financial assistance under Rajiv Gandhi National Fellowship as JRF/SRF (Ref. No. F.14 − 2/2006 (SA-III) to MGS.


Author Information


Malothu Ghan Singh
Department of Botany, Government Degree and PG College, Hyderabad, India