Micropropagation of an ethnomedicinal plant Solanum torvum Swartz


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00502-3
First Page: 1305
Last Page: 1312
Views: 1849


Keywords: Plant growth regulators, Micropropagation, Shoot tip explants, Nodal explants, n In vitro rooting, Plantlet establishment


Abstract


In the current study, we described the impact of various plant growth regulators (PGRs) on the micropropagation of S. torvum (Turkey Berry), a crucial species for ethanomedicine. The explants were cultured on MS medium without PGRs (MSO) as well as MS medium that was fortified with 0.1/0.5 mg/L IAA and various concentrations of BAP/KIN alone. In comparison to KIN alone and IAA + KIN combination, BAP alone and in combination with IAA elicited more numerous shoots in both of the explants of S. torvum that were examined. At 0.1/0.5 mg/L IAA + 1.0–5.0 mg/L BAP/KIN, both explants demonstrated the highest response percentage (80–96%). As opposed to all other concentration combinations of PGRs employed, the highest frequency number of shoots (42.0 ± 0.23) with long (15.2 ± 0.13 cm) shoots were discovered at 0.5 mg/L IAA + 4.0 BAP from shoot tip explants. Thus, it is evident that in the current experiments, shoot tip explants were discovered to have a greater potential for producing a high frequency number of shoots per explant than nodal explants. The elongated micro-shoots produced in both cultures were transferred to root induction medium (RIM), which was supplemented with auxins at concentrations ranging from 0.25 to 2.0 mg/L NAA, IBA, and IAA, for in vitro rooting. At 1.0 mg/L IAA, the highest response rate (90%), the most roots per micro-shoot (17.6 ± 0.15), and the longest roots (8.5 ± 0.16) were seen. In vitro rooted plantlets were placed in sterile vermiculite and soil (1:1) mixture before being adjusted to the greenhouse environment. Plantlets that had been in vitro rooted were transplanted to the field. 90% of the plantlets survived, and their morphology, flowering, and fruiting were all deemed normal. The micropropagation procedure created in the current study can therefore be employed for genetic transformation research to introduce novel genes in the ethnomedicinal plant S. torvum as well as for conservation.


Plant growth regulators, Micropropagation, Shoot tip explants, Nodal explants, n                     In vitro rooting, Plantlet establishment


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References


Aragao FJL, Sarokin L, Vianna GR, Rech EL (2000) Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean (Glycine max (L.) Marril) plant at a high frequency. Theor Appl Genet 101:1–6


Arthan D, Svasti J, Kittakoop P, Pittayakhachonwut D, Tanticharoen M, Thebtaranonth J (2002) Antiviral isoflavonoid sulfate and steroidal glycosides from the fruits of Solanum torvum (Solenaceae). Photochem 39:459–463


Badola KC, Mohinder P, Bhanderi HCS, Pal M(1993) Vegetative propagation of ranbaigan (Solanum torvum Sw.) by rooting branch cuttings. Indian For 119(12): 1,027 – 1,028.


Bais HP, George J, Ravi Shankar GA (2000) In vitro propagation of Decalepis hamiltonii Wight & Arn. an endangered shrub through axillary bud cultures. Curr Sci 79:408–410


Balachandran SM, Bhat SR, Chandel KPS (1990) In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rose). Plant Cell Rep 8:521–524


Bhatt SR, Chandel KPS, Malik SK (1995) Plant regeneration from various explants of cultivated Piper species. Plant Cell Rep 14:398–402


Chah KF, Muko KN, Oboegbulem SI (2000) Antimicrobial activity of methanolic extract of Solanum torvum fruit. Fitoterapia 71:187–189


Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbiolo Reviews 12(4):564–582


CPR Environmental Education Centre (2001) Medicinal plants.


Das TO, Mitra GC (1990) Micropropagation of Eucalyptus tereticornis Smith. Plant Cell Tiss Org Cult 22:95–103


Friedman M, Lee KR, Kim HJ, Lee IS, Kozukue N (2005) Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells. J Agric Food Chem 53:6162–6169


Fui LH (1992) Knowledge and use of forest product as traditional medicine: the case of the forest-dwelling communities. In: Proceedings of the Conference on Medicinal Products from Tropical Rain Forest. K. Shaari, A.A. Kadir and A.R.M. Ali (Eds.). Forest Research Institute of Malaysia. Kuala Lumpur. pp. 385–400





Geetha S, Shetty SA (2000) In vitro propagation of Vanilla planifolia, a tropical orchid. Curr Sci 79:886–889


Ghan Singh M, Rajinikanth M, Rama Swamy N (2021a) Effect of plant growth regulators on somatic embryogenesis and plantlet development of turkey berry (Solanum torvum SW). Eur J Med Plants 32(7):1–8


Ghan Singh M, Rajinikanth M, Rama Swamy N (2021b) High efficiency direct in vitro regeneration from different explants of medicinal plant turkey berry (Solanum torvum Sw). Int Res J Plant Sci 12(5):1–8


Ghan Singh M, Rajinikanth M, Rama Swamy N (2022) High frequency callus mediated plantlet regeneration from different explants of ethno-medicinal plant turkey berry (Solanum torvum Sw). J Sci Res 66(1):121–128


Horzog F, Gautier-Beguin D(2001) Uncultivated plants for human nutrition in Cote d’Ivorie. http://www.fao.org/docrep/W3735e/w3735e10.htm. [20/12/2016]


Kaur R, Mahajan R, Bhardwaj SV, Sharma DR(2000) Meristem tip culture to eliminate strain berry mottle virus from two cultivars of straw berry. Phytomorphology: Int J Plant Morphol 50:192–194


Khartini AW, Dayang Fredalina B, Kaswandi A, Hing HL, Oon KW, Sit NW (2003) Antimicrobial and cytotoxic activities of Holothuria sp. and B. marmorata vitiensis. Malays J Biochem Mol Biol 8:49–53


Lee KR, Kozukue N, Han JS, Park JH, Chang EY, Baek EJ, Chang JS, Friedman M (2004) Glycoalkaloids and metabolites inhibit the growth of human colon (HT29) and liver (HepG2) cancer cells. J Agric Food Chem 52:2832–2839


Liogier HA(1990) Plantas mediciales de puerto rico y del caribe. Iberoamericana de ediciones, Inc San Juan, PR. 566 p


Mackeen MM, Ali AM, Abdullah MA, Nasir RM, Mat NB, Razak AR, Kawazu K (1997a) Antinematodal activity of some Malaysian plant extracts against the pine wood nematode Bursaphelencus xylophilus. Pestic Sci 51:165–170


Mackeen MM, Ali AM, El-Sharkawy SH, Salleh KM, Lajis NH, Kawazu K (1997b) Antimicrobial and cytotoxic properties of some Malaysia traditional vegetables (ulam). Int J Pharmacogn 35:174–178


Mahmood U, Argawal PK, Thakur RS (1985) Torvonin-A, a spirostane saponin from Solanum torvum leaves. Phytochem 24:2456–2457


Maiti PC, Mookherjea S, Matew R, Dan SS (1979) Studies on Indian Solanum I. alkaloid content and detection of solasodine. Econ Bot 33:75–77


Mc Cabe DE, Martinell BJ (1993) Transformation of elite cotton cultivars via particle bombardment of meristems. Biotechnol 11:596–598


Null G (2001) The biochemical activity of plants.


Pattnaik SK, Chand PK (1996) In vitro propagation of medicinal herb Ocimum americanum L. syn O. canum Sims (holy absil) and Ocimum sanctum L. (holy basil). Plant Cell Rep 15:846–850


Purohit SD, Dave A, Kukda G (1994) Micropropagation of safed musli (Chlorophytum borivillianum) a rare medicinal herb. Plant Cell Tiss Org Cult 39:93–96


Rahman MA, Rashid MA, Salam MA, Masud MAT, Masum ASMH, Hossain MM (2002) Performance of some grafted eggplant genotypes on wild Solanum root stocks against root-knot nematode. J Biol Sci 2:446–448


Rama Swamy N, Ugandhar T, Praveen M, Lakshman A, Rambabu M, Venkataiah P(2004) In vitro propagation of medicinally important Solanum surattense. Phytomorphology: Int J Plant Morphol 54:281–289


Rama Swamy N, Ugandhar T, Praveen M, Venkataiah P, Rambabu M, Upender M, Subhash K (2005) Somatic embryogenesis and plantlet regeneration from cotyledon and leaf explants of Solanum surattense. Indian J Biotechnol 4:414–418


Roy SK, Islam MS, Sen J, Hadiuzzaman S (1993) ) Propagation of flood tolerant Jack fruit (Atrocarpus heterophyllus) by in vitro culture. Acta Hort 336:273–278


Royal Horticultural Society (2001) Fruits and vegetables: felty leaves. http://www.rhs.org.uk/about/mn_pubs_journals_garden_0898_aubergine_b.asp. [03/01/2017]


Sahoo Y, Chand PK(1998) In vitro multiplication of medicinal herb. Tridax procumbens L. (maximum diasy, coat bottons). Influence of explanting season, growth regulator synergy. Culture passage and planting substrate. Phytomorphology: Int J Plant Morphol 48:195–205


Sahoo Y, Pattnaik SK, Chand PK (1997) In vitro clonal propagation of an aromatic medicinal herb Ocimum basilicum L. (Sweet basil) by axillary shoot proliferation. In Vitro Cell Dev Biol Plant 33:293–296


Shahzad A, Siddique AS (2000) In vitro organogenesis in Ocimum sanctum (L.)-A multipurpose Herb. Phytomorphology: Int J Plant Morphol 50:27–35


Sharada D, Sai Krishna P, Rama Swamy N (2019) Plant regeneration via somatic embryogenesis in Solanum nigrum L. (Black nightshade) (Solanaceae). Biotech J Inter 23(1):1–9


Silva TMS, Batista MM, Camara CA, Agra MF (2005) Molluscicidal activity of some Brazilian Solanum spp. (Solanaceae) against Biomphalaria glabrata. Ann Trop Med Parasitol 99:419–425


Smitha C, Handique PJ (2000) High frequency in vitro shoot multiplication of Plumbago indica a rare medicinal plant. Curr Sci 78:1187–1188


Sudershan C, Aboel MN, Hussain J (2000) In vitro propagation of Ziziphus mauritiana cultivar umran by shoot tip and nodal multiplication. Curr Sci 80:290–292


Nguelefack TB, Feumebo CB, Ateufack G, Watcho P, Tatsimo S, Atsamo AD, Tane P, Kamanyi A (2008) Anti-ulcerogenic properties of the aqueous and methanol extracts from the leaves of Solanum torvum Swartz (Solanaceae) in rats. J Ethnopharmacol 119(1):135–140

 


Acknowledgements


We thank the University Grants Commission, New Delhi, India for providing the financial assistance under Rajiv Gandhi National Fellowship as JRF/SRF (Ref. No. F.14 − 2/2006 (SA-III) to MGS.


Author Information


Malothu Ghan Singh
Department of Botany, Government Degree and PG College, Hyderabad, India