Molecular marker based diversity of chickpea landraces, wild derivatives and breeding lines using SSR and gene markers

Kumar Tapan1,2, Bharadwaj C


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/2229-4473.2018.00069.1
First Page: 23
Last Page: 29
Views: 194


Keywords: Chickpea, SSR Marker, Diversity, Molecular Variance, Cluster.


Abstract


Fifty chickpea accessions were screened using 102 SSR markers and gene specific primer, which produced a total of 444 alleles. The number of alleles per locus ranged from 02 (ERECTA7, TA110, NC7, H6G01, ICCM0249, TR7, STPK, CAD, AMADH, MYB-TF, TS24, and TA196) to 07 (TA1, SUSI-MEDI-CBF, DREB, DHN, TA206 and TAA94) with an average of 4.3 alleles. PIC ranged from 0.0384 (STPK) to 0.7542(TAA94) with an average of 0.5303. As PIC is a function of allele number and frequency, markers (TA1, SUSI-MEDICBF, DREB, DHN, TA206 and TAA94) with a higher number of alleles generally had higher PIC values and vice versa. Gene diversity ranged from 0.0392 (STPK) to 0.7824 (TAA94) with a mean of 0.5862 in 50 accessions. The genetic variation present in this study has high rate of polymorphism which pointed out towards the scope for further utilization of these markers and varieties for chickpea improvement programmes.


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References



Acknowledgements



Author Information


Kumar Tapan12 Bharadwaj C
1Indian Agricultural Research Institute, Delhi, India-110012

2International Centre for Agricultural Research in the Dry Areas, Madhya Pradesh, India-466114

3Banasthali Vidyapith, Rajasthan, India-304022

*Corresponding author: Indian Agricultural Research Institute, Delhi, India-110012, Email: chbharadwaj@yahoo.co.in