Vegetos

Abstract

Fifty chickpea accessions were screened using 102 SSR markers and gene specific primer, which produced a total of 444 alleles. The number of alleles per locus ranged from 02 (ERECTA7, TA110, NC7, H6G01, ICCM0249, TR7, STPK, CAD, AMADH, MYB-TF, TS24, and TA196) to 07 (TA1, SUSI-MEDI-CBF, DREB, DHN, TA206 and TAA94) with an average of 4.3 alleles. PIC ranged from 0.0384 (STPK) to 0.7542(TAA94) with an average of 0.5303. As PIC is a function of allele number and frequency, markers (TA1, SUSI-MEDICBF, DREB, DHN, TA206 and TAA94) with a higher number of alleles generally had higher PIC values and vice versa. Gene diversity ranged from 0.0392 (STPK) to 0.7824 (TAA94) with a mean of 0.5862 in 50 accessions. The genetic variation present in this study has high rate of polymorphism which pointed out towards the scope for further utilization of these markers and varieties for chickpea improvement programmes.