Vegetos
(An International Journal of Plant Research & Biotechnology)
Parental diversity and hybrids performance for yield related traits in ridge gourd [Luffa acutangula (L.) Roxb.]
Research Articles | Published: 06 June, 2024
First Page: 1598
Last Page: 1608
Views: 2230
Keywords: Parental diversity, Genetic distance, GCA, SCA, Heatmap, RAPD
Abstract
An investigation was carried out to evaluate 5 × 5 half diallel population in the field of BSMRAU following Randomized Complete Block Design (RCBD) with three replications. Molecular diversity was estimated among the parental lines of ridge gourd with eight RAPD markers. Analysis of Molecular Variance (AMOVA) revealed the percentages of molecular variance within population as well as among population as 67% and 33%, respectively. Heatmap illustrated that RG006 exhibited most dissimilarity with RG001, RG003 and RG005. Consequently, Unweighted Neighbor Joining tree clustered the explored parental genotypes of ridge gourd into three major groups. Principle Co-ordinate Analysis (PCoA) revealed maximum genetic distance between RG006 and RG007. The Analysis of Variance (ANOVA) of genotypes, parents and hybrids revealed significant variation for majority of the traits including fruit yield per plant. The hybrid combinations, RG002 × RG007 and RG004 × RG007 were recorded as the best hybrids based on mid and better parent heterosis for the traits viz., days to first female flower appearance, days to first fruit, days to first harvest, first female flower, number of female flower and average fruit weight and RG006 × RG007 has recorded as fruit yield per plant. The hybrids RG002 × RG007 and RG004 × RG006 recorded for best performance of average fruit weight, fruits per plant and fruit yield per plant, respectively and RG006 × RG007 gave the highest fruit yield per plant (632.72 g). Based on the above finding’s, the cross combinations RG002 × RG006, RG002 × RG007, RG004 × RG007 and RG006 × RG007 could be exploited for further evaluation for the development of commercial hybrids with earliness and high yield.
References
Ahsan FN, Islam AKMA, Rasul MG, Mian MAK, Hossain MM (2014) Genetic variability in snake gourd (Tricosanthes cucurminata). J Agric Technol 10(2):355–366
Babicki S, Arndt D, Marcu A, Liang Y, Grant JR, Maciejewski A, Wishart DS (2016) Heatmapper: web-enabled heat mapping for all. Nucleic Acids Res 44(1):147–153
BBS (2021) Statistical year book 2020. Bangladesh Bureau of Statistics, Ministry of Planning, Government of Bangladesh, Dhaka
Belaj A, Satovic Z, Rallo L, Trujillo I (2002) Genetic diversity and relationship in olive germplasm collection as determined by RAPD. Theor Appl Genet 105(4):638–644
Chauhan VBS, Singh DK, Choudhary H, Bansode V, Pati K (2018) Nature of gene action for yield and its contributing traits in sponge gourd (Luffa cylindrica Roem.). Int J Curr Microbiol Applied Sci 7(12):2201–2206
Chuenwarin P, Perez GA, Tongyoo P, Chunwongse J, de Jong H (2020) Genetic diversity and population structure of ridge gourd (Luffa acutangula) accessions in a tropical Asian collection using DArTseq based SNP markers. bioRxiv 3(2):32–33
Devi ND, Mariappan S, Arumugam T, Anandkumar C (2016) Evaluation of snake gourd [Trichosanthes cucumerina L.] genotypes and hybrids. Indian J Hortic 22(2):94–98
Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131(2):479–491
Ferrari CDS, Valente LL, Brod FCA, Tagliari C, Sant’Anna ES, Arisi ACM (2007) Evaluation of polymerase chain reaction and DNA isolation protocols for detection of genetically modified soybean. Int J Food Sci Technol 42(10):1249–1255
Gaur PC, Gupta PK, Kishore H (1978) Studies on genetic divergence in potato. Euphytica 27(2):361–368
Haque MM, Uddin MS, Mehraj H, Uddin AJ (2014) Evaluation of snake gourd (Trichasanthes anguina L) test hybrids comparing with four popular checks. Int J Appl Sci Biotechnol 2(4):525–528
Howe EA, Sinha R, Schlauch D, Quackenbush J (2011) RNA-Seq analysis in MeV. Bioinformatics 27(22):3209–3210
Husna A, Mahmud F, Islam MR, Mahmud MAA, Ratna M (2011) Genetic variability, correlation and path co-efficient analysis in bottle gourd (Lagenaria siceraria L.). Adv Biol Res 5(6):323–327
Iqbal J, Shinwari ZK, Rabbani MA (2014) Investigation of total seed storage proteins of Pakistani and Japanese maize (Zea mays L.) through SDS-PAGE markers. Pak J Bot 46(3):817–822
Jat GS, Munshi AD, Behera TK, Choudhary H, Dev B (2015) Exploitation of heterosis in cucumber for earliness, yield and yield components utilizing gynoecious lines. Indian J Hortic 72(4):494–499
Jolner S, Såstad SM, Taberlet P, Brochmann C (2004) Amplified fragment length polymorphism versus random amplified polymorphic DNA markers: clonal diversity in Saxifraga cernua. Mol Ecol 13(1):81–86
Kashem MA, Islam MM (1999) Use of indigenous agricultural technologies by the rural men and women farmers in Bangladesh. J Sustain Agric 14(2–3):27–43
Khan AMR, Eyasmin R, Rashid MH, Ishtiaque S, Chaki AK (2016) Variability, heritability, character association, path analysis and morphological diversity in snake gourd. Agric Nat Resour 50(6):483–489
Koppad SB, Chavan ML, Hallur RH, Rathod V, Shantappa T (2015) Variability and character association studies in ridge gourd (Luffa acutangula L.) with reference to yield attributes. J Glob Biosci 4(5):2332–2342
Koppad SB, Chavan M, Hallur R (2016) Character association studies and path coefficient analysis for yield and yield attributing traits in Ridge gourd (Luffa acutangula L.). Electron J Plant Breed 7(2):275–281
Lande R (1975) The maintenance of genetic variability by mutation in a polygenic character with linked loci. Genetics Research 26(3):221–235
Manivannan N (2014) TNAUSTAT-Statistical package. Retrived from https://sites.google.com/site/tnaustat. Accessed 19 July 2021
Narasannavar AR, Gasti VD, Shantappa T, Mulge R, Allolli TB, Thammaiah N (2014) Heterosis studies in ridge gourd (Luffa acutangula L.). Karnataka J Agric Sci 27(1):47–51
Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76(10):5269–5273
Perrier X, Jacquemoud-Collet JP (2006) DARwin software v. 6.0. 010.
Podder R, Rasul MG, Islam AKMA, Mian MAK, Ahmed JU (2010) Combining ability and heterosis in snakegourd (Tricosanthes cucurminata L). Bangladesh J Plant Breed Genet 23(2):1–6
Prakash K, Pandey A, Radhamani J, Bisht IS (2013) Morphological variability in cultivated and wild species of Luffa (Cucurbitaceae) from India. Genet Resour Crop Evol 60(8):2319–2329
Rabbani MG, Naher MJ, Hoque S (2012) Variability, character association and diversity analysis of ridge gourd (Luffa acutangula (L.) Roxb.) genotypes of Bangladesh. SAARC J Agric 10(2):1–10
Rai B (1979) Heterosis breeding. Agro-Biological Publications: distributors, Researchco Publications, Berlin
Raihan MS, Newaz MA (2008) Combining ability for quantitative attributes in lablab bean (Lablab purpureus L.). Bangladesh J Plant Breed Genet 21(1):29–34
Raihan HZ, Zaman MRM, Mian MAK (2017) Heterosis in relation to genetic diversity in snake gourd (Tricosanthes cucumerina L.). Bangladesh J Plant Breed Genet 41(1):47–54
Rani EA, Jansirani P, Bapu JK (2017) Assessment of breeding potential of ridge gourd [Luffa acutangula L.] germplasm for growth, yield and quality using diversity (D2) analysis. Int J Curr Microbiol Appl Sci 6(3):128–133
Rehman H, Rabbani MA, Shinwari ZK, Akbar F (2015) RAPD. Markers based genetic diversity of safflower (Carthamus tinctorius L.) germplasm. Pak J Bot 47(6):199–204
Rogers JS (1972) Measures of genetic similarity and genetic distance. Syst Zool 35(5):297–310
Singh PK, Kumar JC, Sharma JR (2000) Genetic estimates (gene action) in long-fruited bottle gourd. J Veg Sci 27(2):162–164
Singh S, Singh VB, Tyagi N (2019) Studies on genetic variability of sponge gourd genotypes. J Pharmacogn Phytochem 8(1):2554–2557
Solieman THI, El-Gabry MAH, Abido AI (2013) Heterosis, potence ratio and correlation of some important characters in tomato (Solanum lycopersicum L.). Sci Hortic 150:25–30
Srikanth D, Ramana CV, Rekha GK, Babu DR, Umakrishna K, Naidu LN (2020) Studies on combining ability for fruit yield and quality attributing characters in ridge gourd (Luffa acutangula Roxb.). J Pharmacogn Phytochem 9(2):1218–1221
Srikanth D, Ramana CV, Rekha GK, Babu DR, Umakrishna K, Naidu LN (2021) Studies on gene action for growth and yield attributing traits in ridge gourd (Luffa acutangula L.). Indian J Hortic 6(3):43–49
Tiessen A, Cubedo-Ruiz EA, Winkler R (2017) Improved representation of biological information by using correlation as distance function for heatmap cluster analysis. Am J Plant Sci 8(3):502–516
Venugopala Reddy M, Patil MG, Kurubar AR, Das U, Patil S (2019) Heterosis studies in sponge gourd for Earliness and qualitative traits. J Pharmacogn Phytochem 8(1):2132–2138
Verma VK, Behera TK, Munshi AD, Parida SK, Mohapatra T (2007) Genetic diversity of ash gourd (Benincasa hispida) inbred lines based on RAPD and ISSR markers and their hybrid performance. Sci Hortic 113(3):231–237
Author Information
Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, Bangladesh