Phylogenetic analysis of selected Asiatic Vigna species based on PCR–RFLP of three non-coding cpDNA Loci

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Research Articles | Published:

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Doi: 10.1007/s42535-022-00493-1
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Keywords: PCR–RFLP, Genetic diversity, Ceratotropis , TrnLUAA intron, TrnLUAA–trnFGAA and psbA-trnHGUG


Abstract


The PCR–RFLP was used to analyze the genetic diversity and relationship between cultivated species and wild taxa belonging to subgenus Ceratotropis of the genus Vigna. The chloroplast DNA was analyzed by amplification followed by restriction of three non-coding regions, trnLUAA intron, trnLUAAtrnFGAA and psbA-trnHGUG spacers. The length of intergenic spacers trnLUAAtrnFGAA and psbA-trnHGUG varied from 475 to 580 bp. The length of trnLUAA intron varied from 600 to 700 bp. Wild forms V. radiata var. sublobata and V. mungo var. silvestris emerged as distinct taxa and grouped close to their proposed cultivated forms viz. V. radiata and V. mungo. V. radiata var. setulosa, another wild form grouped closer to V. radiata var. sublobata and V. radiata. More importantly, V. hainiana appeared to be closely related to V. mungo and V. radiata, therefore needs to be investigated for its role in the domestication of these two important pulse yielding taxa.


PCR–RFLP, Genetic diversity, 
              Ceratotropis
            , 
                     TrnLUAA intron, 
                     TrnLUAA–trnFGAA and psbA-trnHGUG


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Acknowledgements



Author Information


Vir Ruchi
Zakir Husain Delhi College Department of Botany, Delhi University, Delhi, India
ruchivir@zh.du.ac.in
Jehan Tabassum
Zakir Husain Delhi College Department of Botany, Delhi University, Delhi, India


Bhat K. V.
National Research Centre on DNA Fingerprinting, National Bureau of Plant Genetic Resources, New Delhi, India


Lakhanpaul S.
Department of Botany, Delhi University, Delhi, India