Production and optimisation of cellulase enzyme by Aspergillus uvarum CBS 121591 isolated from soil

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00450-y
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Keywords: Cellulase, Isolation, Aspergillus uvarum , Optimisation, Submerged fermentation


Abstract


Cellulases are tremendously significant enzymes at both industrial and environmental level, as they perform a vital part in the earth’s carbon chain by causing breakdown of composite cellulose into simple sugar form. In this study, isolation of the cellulose degrading fungal strains was done from the soil samples collected from Banasthali Vidyapith. Fungal cultures formed hydrolysis zone on the carboxymethyl cellulose (CMC) agar plate after staining with 0.1% w/v Congo red dye hence, were recognized as cellulolytic fungi. Cellulolytic enzyme production was performed using submerged fermentation (SmF). Fungus exhibited relatively high cellulase activity (2.706 U/ml) after 72 h of incubation. Various culture elements for instance incubation time, carbon and nitrogen source, pH, temperature, inoculum size and agitation speed were optimized for the optimum enzyme production. The cellulase production was optimum after 72 h of incubation, when CMC and yeast extract were taken as source of carbon and nitrogen respectively at a concentration of 1.5% w/v, pH 7.0, temperature at 37 °C, inoculum size of 1.5% v/v having spore count 108 ml−1 and agitation speed of 100 rpm.


Cellulase, Isolation, 
              Aspergillus uvarum
            , Optimisation, Submerged fermentation


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Acknowledgements


The authors are grateful to the Head, Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan for giving necessary lab amenities.


Author Information


Bhati Nikita
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Jaipur, India

Shreya
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Jaipur, India


Sharma Arun Kumar
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Jaipur, India

arun.k.sharma84@gmail.com