Production and optimisation of cellulase enzyme by Aspergillus uvarum CBS 121591 isolated from soil

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00450-y
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Keywords: Cellulase, Isolation, Aspergillus uvarum , Optimisation, Submerged fermentation


Cellulases are tremendously significant enzymes at both industrial and environmental level, as they perform a vital part in the earth’s carbon chain by causing breakdown of composite cellulose into simple sugar form. In this study, isolation of the cellulose degrading fungal strains was done from the soil samples collected from Banasthali Vidyapith. Fungal cultures formed hydrolysis zone on the carboxymethyl cellulose (CMC) agar plate after staining with 0.1% w/v Congo red dye hence, were recognized as cellulolytic fungi. Cellulolytic enzyme production was performed using submerged fermentation (SmF). Fungus exhibited relatively high cellulase activity (2.706 U/ml) after 72 h of incubation. Various culture elements for instance incubation time, carbon and nitrogen source, pH, temperature, inoculum size and agitation speed were optimized for the optimum enzyme production. The cellulase production was optimum after 72 h of incubation, when CMC and yeast extract were taken as source of carbon and nitrogen respectively at a concentration of 1.5% w/v, pH 7.0, temperature at 37 °C, inoculum size of 1.5% v/v having spore count 108 ml−1 and agitation speed of 100 rpm.

Cellulase, Isolation, 
              Aspergillus uvarum
            , Optimisation, Submerged fermentation


Bhati N, Sharma AK (2021) Cost-effective cellulase production, improvement strategies, and future challenges. J Food Process Eng 44(2):e13623.

Brás JL, Cartmell A, Carvalho ALM et al (2011) Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis. PNAS 108(13):5237–5242.

Chandel K, Jandaik S, Kumari V, Sarswati S, Sharma A, Kumar D, Kumar N (2013) Isolation, purification and screening of cellulolytic fungi from mushroom compost for production of enzyme (cellulase). Int J Curr Res 5(1):222–229

Deejing S, Dittamart D (2015) Isolation and screening of cellulase-producing microorganisms and the study of some characteristics of enzymes. In: Biology education and research in a changing planet, Springer, Singapore, pp 157–166.

Devanathan G, Shanmugan T, Balasubramanian T, Manivannan S (2007) Cellulase production by Aspergillus niger isolated from coastal mangrove debris. Trends Appl Sci Res 2(1):23–27.

Effiong TE, Abdulsalami MS, Egbe NE, Bakare V (2019) Screening of fungi isolates from soil, pulp waste water and rotten wood for cellulase producing potentials. J Appl Sci Environ Manag 23(6):1051–1055.

El-Hadi AA, El-Nour SA, Hammad A, Kamel Z, Anwar M (2014) Optimization of cultural and nutritional conditions for carboxymethylcellulase production by Aspergillus hortai. J Radiat Res Appl Sci 7(1):23–28.

Ganguly R, Mukherjee SK (1995) Effects of different pure and complex carbon and nitrogen sources on production of cellulases by an isolated strain Penicillium purpurogenum. J Microb Biotechnol 10(1):47–58

Gautam SP, Bundela PS, Pandey AK, Khan J, Awasthi MK, Sarsaiya S (2011) Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. Biotechnol Res Int 2011:1–8.

Gomathi D, Muthulakshmi C, Kumar DG, Ravikumar G, Kalaiselvi M, Uma C (2012) Submerged fermentation of wheat bran by Aspergillus flavus for production and characterization of carboxy methyl cellulase. Asian Pac J Trop Biomed 2(1):S67–S73.

Gomes I, Gomes J, Gomes DJ, Steiner W (2000) Simultaneous production of high activities of thermostable endoglucanase and β-glucosidase by the wild thermophilic fungus Thermoascus aurantiacus. Appl Microbiol Biotechnol 53(4):461–468.

Goukanapalle PKR, Kanderi DK, Rajoji G, Kumari S, Bontha RR (2020) Optimization of cellulase production by a novel endophytic fungus Pestalotiopsis microspora TKBRR isolated from Thalakona forest. Cellulose 27(11):6299–6316.

Hankin L, Anagnostakis SL (1977) Solid media containing carboxymethylcellulose to detect Cx cellulase activity of micro-organisms. Microbiology 98(1):109–115.

Haq I, Iqbal SH, Qadeen MA (1993) Production of xylanase and CMC cellulase by mold culture. Pak J Biotechnol 4:403–409

Hayward TK, Hamilton J, Tholudur A, McMillan JD (2000) Improvements in titer, productivity, and yield using solka-floc for cellulase production. In: Twenty-first symposium on biotechnology for fuels and chemicals. Applied biochemistry and biotechnology Humana Press, Totowa, NJ, pp 859–874.

Herculano PN, Porto TS, Moreira KA, Pinto GA, Souza-Motta CM, Porto ALF (2011) Cellulase production by Aspergillus japonicus URM5620 using waste from castor bean (Ricinus communis L.) under solid-state fermentation. Appl Biochem Biotechnol 165(3):1057–1067.

Ibrahim ASS, El-diwany AI (2007) Isolation and identification of new cellulases producing thermophilic bacteria from an Egyptian hot spring and some properties of the crude enzyme. Aust J Basic Appl Sci 1(4):473–478

Janveja C, Rana SS, Soni SK (2014) Optimization of valorization of biodegradable kitchen waste biomass for production of fungal cellulase system by statistical modelling. Waste Biomass Valoriz 5(5):807–821.

Julia BM, Belén AM, Georgina B, Beatriz F (2016) Potential use of soybean hulls and waste paper as supports in SSF for cellulase production by Aspergillus niger. Biocatal Agric Biotechnol 6:1–8.

Krishna C (1999) Production of bacterial cellulases by solid state bioprocessing of banana wastes. Bioresour Technol 69(3):231–239.

Liang YL, Zhang Z, Wu M, Wu Y, Feng JX (2014) Isolation, screening, and identification of cellulolytic bacteria from natural reserves in the subtropical region of China and optimization of cellulase production by Paenibacillus terrae ME27-1. Biomed Res Int 2014:1–13.

Lu W, Li D, Wu Y (2003) Influence of water activity and temperature on xylanase biosynthesis in pilot-scale solid-state fermentation by Aspergillus sulphureus. Enzyme Microb Technol 32(2):305–311.

Sari SLA, Setyaningsih R, Wibowo NFA (2017) Isolation and screening of cellulolytic fungi from Salacca zalacca leaf litter. Biodiversitas 18(3):1282–1288.

Mahdi SN, Mohd N, Arbakariya A, Rosfarizan M, Shuhaimi M (2010) Optimization of cellulase production by Aspergillus terreus under submerged fermentation using response surface methodology. Aust J Basic Appl Sci 4(12):6106–6124

Mahdi S, Mohd N, Arbakariya A, Rosfarizan M (2011) Screening, isolation and selection of cellulolytic fungi from oil palm empty fruit bunch fibre. Biotechnol 10(1):108–113.

Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426–428.

Prasanna HN, Ramanjaneyulu G, Rajasekhar Reddy B (2016) Optimization of cellulase production by Penicillium sp. 3 Biotech 6(2):1–11.

Saha S, Roy RN, Sen SK, Ray AK (2006) Characterization of cellulase-producing bacteria from the digestive tract of tilapia, Oreochromis mossambica (Peters) and grass carp, Ctenopharyngodon idella (Valenciennes). Aquacult Res 37(4):380–388.

Satyamurthy P, Jain P, Karande VS, Nadanathangam V (2016) Nanocellulose induces cellulase production in Trichoderma reesei. Process Biochem 51(10):1452–1457

Sethi S, Datta A, Gupta BL, Gupta S (2013) Optimization of cellulase production from bacteria isolated from soil. Int Sch Res Not 2013:1–7.

Shanmugapriya K, Saravana PS, Krishnapriya MM, Mythili A, Joseph S (2012) Isolation, screening and partial purification of cellulase from cellulase producing bacteria. Int J Adv Biotechnol Res 3(1):509–514



The authors are grateful to the Head, Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan for giving necessary lab amenities.

Author Information

Bhati Nikita
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Jaipur, India

Department of Bioscience and Biotechnology, Banasthali Vidyapith, Jaipur, India

Sharma Arun Kumar
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Jaipur, India