Quantitative analysis and assessment of anti-oomycetes action against Phytophthora infestans through biomolecules from Bacillus firmus HussainT:Lab.66 through mass spectrometry

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Doi: 10.1007/s42535-024-00849-9
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Keywords: n Bacillusn , Biosurfactant, Lipopeptide, n Phytophthora infestansn , Potato


Abstract


Bacterial biomolecules will be key ingredients of future sustainable crop production. Metabolites from an improved strain of Bacillus sp., collected from waste soil, were analyzed for bioactivity to produce biofilm and mobility and as antifungal factors. The analysis determined the presence of the biosurfactant surfactin in high amounts. Fourier transform infrared spectroscopy and gas chromatography–mass spectrometry indicated the presence of high amounts of methyl ester fatty acids in the extract. The lipopeptide extract of the Bacillus firmus HussainT:Lab.66 strain exhibited results with haemolytic activity (2.0 cm), BATH assay (94%), oil spread assay (2.1 cm) and EI 24% (95%). These activities are due to hydrophilic and hydrophobic residues that are partitioned at the liquid/liquid, liquid/gas, or liquid/solid interfaces, demonstrating their potential to produce surfactants. Under in vitro studies, the supernatant exhibited good antifungal activity against Phytophthora infestans, in which mycelial growth was inhibited by > 50% at 3% concentration. The new potent strain isolated in this study could be a new biological control agent and a way to combat plant diseases in a bio-rationale manner and minimize the need for synthetic chemicals.



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Acknowledgements


The author is thankful to SERB-Dept. of Science and Technology, Govt. of India, New Delhi, for providing financial assistance under File No. SERB/DST-NPDF/2016/001409 and Dept. of Botany, Aligarh Muslim University, Aligarh, U.P, for providing the laboratory infrastructure support. The authors thank the Dept. of Chemistry, Instrumentation laboratory, AMU, Aligarh for providing access to the FT-IR facility and Advanced Instrumentation Research Facility, at Jawaharlal Nehru University, New Delhi, for GC-MS facility, Sophisticated Analytical Instrumental Facility Centre for LC-MS, at CSIR-CDRI, Lucknow.


Author Information


Hussain Touseef
Plant Pathology Section, Department of Botany, Aligarh Muslim University, Aligarh, India
Hussaintouseef@yahoo.co.in