Rapid propagation of Celastrus paniculatus Willd.: an endangered medicinal plant through indirect organogenesis


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-020-00105-w
First Page: 277
Last Page: 285
Views: 824

Keywords: Celastrus paniculatus Willd., Indirect organogenesis, meta–topollin, Shoot multiplication, Growth regulators


A rapid and efficient propagation system through callus explant derived from leaf was established in Celastrus paniculatus Willd., a medicinal plant of the Celastraceae family. Seed dormancy and vegetative propagation render it for developing an in vitro regeneration method. Murashige and Skoog (MS) media containing 6-benzylaminopurine (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthalene acetic acid (NAA) and with various concentrations of BAP + NAA, BA + 2,4-D and BAP + IAA produced different natures of calli. Moreover, BAP + NAA produced friable callus, whereas BAP + 2, 4-D produced compact calli, which were transferred to the shoot initiation medium containing BAP supplemented with ascorbic acid and each of adenine sulfate, arginine and citric acid. Inclusion of meta-topolin in the media along with optimum concentration of BAP promoted shoot multiplication and elongation after 8 weeks of culture. The in vitro elongated shoots were treated with different auxins such as IAA (Indole 3 Acetic Acid), IBA (Indole-3-butyric acid) and NAA (Naphthalene acetic acid) individually for early rooting and the treated shoots were transferred to the half-strength MS medium. The regenerated plantlets were acclimatized in pots containing sterilized soil and sand and then transferred to the field conditions, 90% of the regenerants survived. Thus, this was the first report on indirect organogenesis of C paniculatus Willd. using callus explant obtained from direct organogenesis leaf.

Celastrus paniculatus Willd., Indirect organogenesis, meta–topollin, Shoot multiplication, Growth regulators

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  1. Ahmad S, Spoor W (1999) Effect of NAA and BAP on callus culture and plant regeneration in Curly kale (Brassica oleraces L.). Pak J Biol Sci 2:109–112

  2. Ahmad N, Faisal M, Anis M, Aref IM (2010) In vitro callus induction and plant regeneration from leaf explants of Ruta graveolens L. S Afr J Bot 76:597–600

  3. Ahmed MR, Anis M, Alatar AA, Faisal M (2017) In vitro clonal propagation and evaluation of genetic fidelity using RAPD and ISSR marker in micropropagated plants of Cassia alata L.: a potential medicinal plant. Agrofor Syst 91:637–647

  4. Anil KM, Ranjitha Kumari BD (2019) Direct regeneration of plantlets from shoot tip explants of a vulnerable medicinal plant—Celastrus paniculatus Willd. J Appl Hortic 21:189–194

  5. Anusha TS, Joseph MV, Elyas KK (2016) Callus induction and elicitation of total phenolics in callus cell suspension culture of Celastrus paniculatus–willd, an endangered medicinal plant in India. Pharmacogn Res 8(5):471–475

  6. Arya V, Singh RP, Shekhawat NS (2001) A micropropagation protocol for mass multiplication and off-site conservation of Celastrus paniculatus—a vulnerable medicinal plant of India. J Sustain Forest 14:107–120

  7. Asad M, Ahmed N, Sohail A, Sher J, Burni T, Hadi F (2019) In vitro callus induction and plantlet regeneration of sesame (Sesamum Indicum L.). Pure Appl Biol (PAB) 8:1307–1313

  8. Bairu MW, Stirk WA, Dolezal K, Van Staden J (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell Tissuse Org Cult 90:15–23

  9. Bairu MW, Stirk WA, Dolezal VanStaden J (2008) The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA). Plant Cell Tissuse Org Cult 95:373–379

  10. Bakar DA, Ahmed BA, Taha RM (2014) In vitro callus induction and plant regeneration of Celosia argentea—an important medicinal plant. Braz Arch Biol Technol 57:860–866

  11. Chaturvedi HC, Sharma M (1989) In vitro production of cloned plants of jojoba (Simmondsia chinensis (Link) Schneider) through shoot proliferation in long-term culture. Plant Sci 63:199–207

  12. Dakshayini K, Vaman CR, Anitha K, Bhavyashree V, Ujwal P (2016) Highrfrequency of plant regeneration and histological analysis of calus in Cichorium intybus: an important medicinal plant. J Phytol 8:7–12. https://doi.org/10.19071/jp.2016.v8.2980

  13. Dhavala A, Rathore TS (2010) Micropropagation of Embelia ribes Burm f. through proliferation of adult plant axillary shoots. In Vitro Biol Plant 46:180–191

  14. Dreger M, Mol R, Deja A, Raj E, Mańkowska G, Wielgus K (2019) Improved plant regeneration in callus cultures of Sorghum bicolor (L.) Moench. In Vitro Biol Plant 55:190–198

  15. Handa S (1998) Indian herbal pharmacopoeia vol-II Celastrus paniculatus. IDMA Mumbai 7:26–34

  16. Jang HR, Lee HJ, Park BJ, Pee OJ, Paek KY, Park SY (2016) Establishment of embryogenic cultures and determination of their bioactive properties in Rosa rugosa. Horticult Environ Biotechnol 57:291–298

  17. Jose SC, Sivaraman K, Singh HP (2001) Medicinal and aromatic plants. Floricult Today 24–32

  18. Kubalakova M, Strnad M (1992) The effect of aromatic cytokinins (populins) on and regeneration of sugar beet. In Vitro Biol Plant 34:578–579

  19. Kumar M, Gupta Y (2002) Antioxidant property of Celastrus paniculatus Willd.: a possible mechanism in enhancing cognition. Phytomedicine 9:302–311

  20. Kumar A, Aggarwal D, Gupta P, Reddy MS (2010) Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum. Biol Plant 54:601–606

  21. Lal D, Singh N (2010) Mass multiplication of Celastrus paniculatus Willd—an important medicinal plant under in vitro conditions using nodal segments. Am J Sci 6:55–61

  22. Maity S, Ray S, Banerjee N (2005) The role of plant growth regulators on direct and indirect plant regeneration fromvarious organs of Leucaena leucocephala. Acta Physiol Plant 27:473–840

  23. Martin G, Geetha SP, Raja SS, Raghu AV, Balachandran I, Ravindran PN (2006) An efficient micropropagation system for Celastrus paniculatus Willd.: a vulnerable medicinal plant. J Forest Res 11:461–465

  24. Mridula K, Parthibhan S, Kumar TS, Rao MV (2017) In vitro organogenesis from Tinospora cordifolia (Willd.) Miers—a highly valuable medicinal plant. S Afr J Bot 113:84–90

  25. Nadgauda RS, Mascarenhas AF, Hendre RR, Jagannathan V (1978) Rapid multiplication of turmeric (Curcuma longa Linn.) plants by tissue culture. Indian J Exp Biol 16:120–122

  26. Nathar VN, Yatoo GM (2014) Micropropagation of an antidiabetic medicinalplant, Artemisia pallens. Turk J Botany 38:491–498

  27. Parrotta JA (2001) Healing plants of peninsular India. CABI Publishing, New York

  28. Pathak A, Joshi A, Shrivastava N, Sharma P (2017) Regeneration and chemical profiling in Hemidesmus indicus (L.) R. Br. S Afr J Bot 113:413–420

  29. Raha S, Roy SC (2001) In vitro plant regeneration in Holarrhena antidysenterica wall, through high-frequency axillary shoot proliferation. In Vitro Biol Plant 37:232–236

  30. Rajesekharan P (2002) Conservation of medicinal plant biodiversity—an Indian perspective. J Med Arom Plant Sci. 24:132–147

  31. Rao MS, Purohit SD (2006) In vitro shoot bud differentiation and plantlet regeneration in Celastrus paniculatus Willd. Biol Plant 50:501–506

  32. Sarasan V, Cripps R, Ramsay MM, Atherton C, McMichen M, Prendergast G, Rowntree JK (2006) Conservation in vitro of threatened plants—progress in the past decade. In Vitro Biol Plant 42:206–214

  33. Senapati SK, Aparajita S, Rout GR (2013) Micropropagation and assessment of genetic stability in Celastrus paniculatus: an endangered medicinal plant. Biologia 68:627–632

  34. Sharada M, Ahuja A, Kaul MK (2003) Regeneration of plantlets via callus cultures in Celastrus paniculatus Willd—a rare endangered medicinal plant. J Plant Biochem Biot 12:65–69

  35. Phulwaria M, Rai MK, Patel AK, Kataria V, Shekhawat, NS (2013) A genetically stable routing protocol for propagating a threatened medicinal plant—Celastrus paniculatus. AoB Plants 5:1–9

  36. Siddique I, Anis M, Aref IM (2010) In vitro adventitious shoot regeneration via indirect organogenesis from petiole explants of Cassia angustifolia Vahl.—a potential medicinal plant. Appl Biochem Biotechnol 162:2067–2074

  37. Singh J, Tiwari KN (2012) In vitro plant regeneration from decapitated embryonic axes of Clitoria ternatea L.—an important medicinal plant. Ind Crops Prod 35:224–229

  38. Singh S, Banerjee M, Kumar M (2017) An efficient protocol for plant regeneration of Phlogacanthus thyrsiflorus Nees: an important medicinal shrub. In: Applications of biotechnology for sustainable development. Springer, Singapore, pp 15–20

  39. Singh M, Bhatti S, Verma SK (2019) Improved plant regeneration method of Artocarpus lakoocha Roxb. from immature seeds. Vegetos 32:269–274

  40. Subbaiyan B, Samydurai P, Prabu MK, Ramakrishnan R, Thangapandian V (2014) Inventory of rare, endangered and threatened (RET) plant species in maruthamalai hills, western ghats of Tamilnadu, South India. Our Nat 12:37–43

  41. Tantikanjana T, Yong JW, Letham DS, Griffith M, Hussain M, Ljung K, Sundaresan V (2001) Control of axillary bud initiation and shoot architecture in Arabidopsis through the SUPERSHOOT gene. Genes Dev 15:1577–1588

  42. Valero-Aracama C, Kane ME, Wilson SB, Philman NL (2010) Substitution of benzyladenine with meta-topolin during shoot multiplication increases acclimatization of difficult-and easy-to-acclimatize sea oats (Uniola paniculata L.) genotypes. Plant Growth Regul 60:43

  43. Verma AK, Prasad KV (2019) Organogenesis and anatomical study of gamma rays induced mutant of chrysanthemum (Chrysanthemum morifolium Ramat.) from ray florets. Res J Biotechnol 3:14:3

  44. Warrier PK, Nambiar V (1993) Indian medicinal plants: a compendium of 500 species. Orient Blackswan, Hyderabad

  45. Werbrouck SPO, van der Jeugt B, Dewitte W, Prinsen E, van Onckelen HA, Debergh PC (1995) The metabolism of benzyladenine in S. floribundum Schott ‘Petite’ in relation toacclimatisation problems. Plant Cell Rep 14:662–665

  46. Wojtania A (2010) Effect of meta-topolin in vitro propagation of Pelargonium × hortorum and Pelargonium × hederaefolium cultivars. Acta Soc Bo Pol 79:101–106






We thank CSIR for providing the Senior Research Fellowship to Anil Kumar Moola vide sanction letter number 09/475(0201)/2018–EMR–I.

Author Information

Moola Anil Kumar
Department of Botany, Bharathidasan University, Tiruchirappalli, India

Kumari B. D. Ranjitha
Department of Botany, Bharathidasan University, Tiruchirappalli, India