Vegetos
(An International Journal of Plant Research & Biotechnology)
RD29A-IPT expression enhances drought tolerance in transgenic perennial ryegrass
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Research Articles | Published: 27 April, 2025
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Keywords: Accession, Isopentenyl adenosine, IPT expression, Lolium, Photochemical efficiency, RD29A promoter
Abstract
Genetic improvement and the identification of drought-tolerant cultivars are crucial in perennial ryegrass (Lolium perenne L.) turfgrass to enhance germplasm reserves for molecular breeding and the development of sustainable landscapes in arid and semi-arid green spaces. Cytokinins (CKs) are plant hormones that regulate various physiological processes, including cell division, shoot growth, and leaf senescence, and also are known to regulate plant responses to drought stress. This study aimed to enhance drought tolerance in perennial ryegrass cultivars by utilizing the drought-inducible RD29A promoter to drive the expression of the IPT gene, which boosts cytokinin levels. The research also compared the performance of these transgenic plants with wild-type (WT) plants and local perennial ryegrass accessions under varying irrigation conditions. Results showed that certain transgenic plants and local accessions displayed higher drought tolerance based on turf quality, physiological, and biochemical characteristics. The expression of the IPT gene was confirmed in transgenic plants exposed to drought stress. Transgenic lines including GM24, GM12, GC8, GC6, NC12, NC14, NS14, and GC3 exhibited increased drought tolerance, maintaining higher levels of cytokinins in the leaves, improving water content, photosynthetic rate, and antioxidant activity while reducing damage indicators. Catalase and superoxide dismutase activities were more influential than peroxidase in drought adaptation and recovery. The efficiency of the RD29A promoter and the use of the UBQ10 intron in the IPT gene construct affected gene expression. Moreover, the significant genotypic variation among local accessions indicates opportunities for improving drought tolerance through targeted breeding in sensitive and moderately tolerant genotypes. Further research is recommended to investigate hormonal balance and osmoregulation in transgenic and wild-type plants under multiple stresses. Additionally, identifying candidate genes involved in drought tolerance, particularly in local ryegrass accessions, should be a focus for future studies.
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Author Information
Department of Horticultural Science, School of Agriculture, Shiraz University, Shiraz, Iran