Regenerative Competence in Root explants of Medicinally important Orchid Cymbidium aloifolium: An in vitro study

Verma Shivani*, Pathak Promila

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/2229-4473.2018.00014.9
First Page: 100
Last Page: 103
Views: 1281


Keywords: <I>Cymbidium aloifolium</I>, PGRs, Callus, Organogenesis, Hardening.


Abstract

The paper reports the possibility of using root explants for micropropagating Cymbidium aloifolium. Mitra et al. (1976) medium supplemented with 2, 4-D (2mgl−1) induced callusing at the root tip. However, cent% explants responded via shoot bud formation within 13.25 ± 0.95 days. The neoformations organogenated subsequently. The plantlets grown on M medium supplemented with NAA (3mgl−1) and those with 4–5 leaves and a number of roots and further these were subjected to a two-step hardening procedure by subjecting them to in vitro and ex vitro hardening. These survived with 60% frequency.


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References


Acknowledgements


Author Information

Verma Shivani* Pathak Promila
Orchid laboratory, Panjab University, Chandigarh-160 014, India

*Corresponding author: Orchid laboratory, Panjab University, Chandigarh-160 014, India, Email: shiv.ni.verma@gmail.com