Reproductive ecology of Epilobium hirsutum L. an invasive alien species in Kashmir Himalaya

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00357-8
First Page: 910
Last Page: 922
Views: 106


Keywords: Epilobium hirsutum , Invasive, Pollen biology, Breeding behavior


Abstract


Invasive plants are an issue of great concern globally. The concerned plant species threaten native biodiversity and cause ecological and economic damage. They develop different reproductive strategies to perpetuate in different environments. Hence insights into reproductive biology are necessary for developing different conservation protocols. The present study was carried out to determine the complete reproductive biology of the E. hirsutum, an invasive alien plant species of Kashmir Himalaya growing at an altitudinal range of 1500–2700 m.a.s.l. As revealed by the present study, seed was the main propagule for the spread of the species, because the percent seed set and percent seed viability were found to be high in KUBG population (95.13% and 93.3%, respectively). The highest percentage germination was found in seeds treated with 25 ppm IAA (100%). The decline in MGT was observed in seeds treated with 25 and 50 ppm Thiourea. The results revealed the highest pollen ovule ratio in Shopian population (30.01 ± 3.25) and the lowest in the Shalimar population (26.92 ± 27). Moreover pollen germination was found maximum in a medium containing sucrose, boric acid, calcium nitrate, potassium nitrate, and magnesium sulphate in the ratio of 1:1 (46.1%). Stigma was found to be most receptive during the fifth day after anthesis (24.89%). The species show mixed mating strategies. The highest percent seed set was found in cross-pollinated flowers (74.58%) followed by geitonogamy (43.35%). Based on the insect visiting efficiency the Bracon sp. was found to be the main pollinator of the species with a visiting efficiency of (0.09 ± 0.0). Our results provide a detailed account of the reproductive biology of the species which help us to predict its potential for future spread. Maximum seed viability, outcrossing nature of the species, generalist large pollinator species, and maximum germination potential of seeds are some of the reproductive attributes which are the main causes for the spread of the species.



                Epilobium hirsutum
              , Invasive, Pollen biology, Breeding behavior


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References


Abdul-Baki A, Anderson JD (1973) Vigor determination in Soyabean seed by multiple criteria. Crop Sci 13:630–633


Agarwal PK, Dadlani (1992) Techniques in seed science and technology. South Asian Publication


Baker HG (1965) Characteristics and modes of origin of weeds. In: Baker G, Stebbins GL (eds) The genetics of colonizing species. Academic Press, New York, pp 147–172


Barrett SCH, Colautti RI, Eckert CG (2008) Plant reproductive systems and evolution during biological invasion. Mol Ecol 17:373–383


Barrett SC (2011) Why reproductive systems matter for the invasion biology of plants. In: Fifty years of invasion ecology, the legacy of Charles Elton, vol 1, pp 195–210


Bartomeus I, Bosch J, Vilà M (2008) High invasive pollen transfer, yet low deposition on native stigmas in a Carpobrotus-invaded community. Ann Bot 102:417–424


Bingham RA, Orthner AR (1998) Efficient pollination of alpine plants. Nature 391:238–239. https://doi.org/10.1038/34564


Brewbaker JL, Kwack BH (1963) The essential role of calcium ion in Pollen germination and pollen tube growth. Am J Bot 50:859–865. https://doi.org/10.1002/j.1537-2197.1963.tb06564.x


Brown BJ, Mitchell RJ, Graham SA (2002) Competition for pollination between an invasive species (purple loosestrife) and a native congener. Ecology 83:2328–2336


Carnoy JB (1886) La cytodierèse de l’oeuf. Cellule 3:1–92


Chittka L, Schürkens S (2001) Successful invasion of a floral market. Nature 411:653–653


Cruden RW (1977) Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plants. Evolution 31:32–46. https://doi.org/10.2307/2407542


Dafni A, Firmage D (2000) Pollen viability and longevity: practical, ecological and evolutionary implications. Pollen Pollination 222:113–132


Datta R, Chourey PS, Pring DR, Tang HV (2001) Gene-expression analysis of sucrose-starch metabolism during pollen maturation in cytoplasmic male-sterile and fertile lines of sorghum. Sex Plant Reprod 14:127–134


Erdtman G (1952) Pollen morphology and plant taxonomy—angiosperms. Almqvist and Wiksell, Stockholm, p 539


Forman J, Kesseli RV (2003) Sexual reproduction in the invasive species Fallopia japonica (Polygonaceae). Am J Bot 90:586–592. https://doi.org/10.3732/ajb.90.4.586


Gross Y, Kigel J (1994) Differential sensitivity to high temperatures of stages in the reproduction development of common beans (Phaseolus vulgaris L.). Field Crop Res 36:201–2012


Haynes KF (1988) Sublethal effects of neurotoxic insecticides on insect behaviour. Annu Rev Entomol 33:149–168. https://doi.org/10.1146/annurev.en.33.010188.001053


Hendry GA, Grime JP (1993) Methods in comparative plant ecology. Chapman and Hall


Hooker JD (1881) The flora of British India. Ashford 3:291–297


Hulme PE (2006) Beyond control: wider implications for the management of biological invasions. J Appl Ecol 43:835–847


Jairui C, Hoch PC, Raven PH (2007) Epilobium Linneaus. Flora China 13:411–423


Joshi M, Dhar U (2003) Effect of various pre sowing treatments on seed germination of Heracleum candicans Wall ex. DC: a high value medicinal plant. Seed Sci Technol 31:737–743. https://doi.org/10.15258/sst.2003.31.3.22


Kant V, Vashist DP (1998) Effect of GA3 and storage on germination of Nardostachys jatamansi seeds. J Hill Res 11:202–206


Karni L, Aloni B (2002) Fructo kinase and hexokinase from pollen grains of bell pepper (Capsicum annuum L.): possible role in pollen germination under conditions of high temperature and CO2 enrichment. Ann Bot 90:607–612


Kaufman PB, Carlson TF, Dayanandan P, Evaus ML, Fisher JB, Parks C, Wells JR (1989) Plants, their biology and importance. Hoppe and Raw Publishers, New York, pp 714–730


Kaul MK (1986) Weed flora of Kashmir Valley. Scientific Publishers, Jodhpur, India


Khuroo AA, Rashid I, Reshi Z, Dar GH, Wafai BA (2007) The alien flora of Kashmir Himalaya. Biol Invasions 9:269–292


Lawrance HM (1951) Taxonomy of vascular plants. The Macmillan Company, New York, pp 670–672


Leung BD, Lodge M, Finnoff D, Shogren JF, Lewis MA, Lamberti G (2002) An ounce of prevention or a pound of cure: bioeconomic risk analysis of invasive species. Proc Roy Soc Lond B 269:2407–2413


Lubbers AE, Christensen NL (1986) Intraseasonal variation in seed production among flowers and plants of Thalictrum thalctroides (Ranunculaceae). Am J Bot 73:190–203. https://doi.org/10.1002/j.1537-2197.1986.tb08520.x


Nath R (1996) Comprehensive college botany, vol 11. Kalyani Publisher, New Delhi, pp 57–98








Pressman E, Peet MM, Pharr DM (2002) The effect of heat stress on tomato pollen characteristics is associated with changes in carbohydrate concentration in the developing anthers. Ann Bot 90:631–636


Pyšek P, Richardson DM (2007) Traits associated with invasiveness in alien plants: where do we stand? In: Nentwig W (ed) Biological invasions. Springer, Berlin, pp 97–125


Rejmánek M, Richardson DM, Higgins SI, Pitcairn MJ, Grotkopp E (2005) Ecology of invasive plants: state of the art. In: Mooney HA, Mc Neely JA, Neville L, Schei PJ, Waage J (eds) Invasive alien species: searching for solutions. Island Press, Washington, DC


Rice WR, Sax DF (2005) Testing fundamental evolutionary questions at large spatial and demographic scales: Species invasions as an underappreciated tool. In: Sax DF, Stachowicz JJ, Gaines SD (eds) Species invasions: insights into ecology, evolution, and biogeography. Sinauer Associates Inc., Sunderland, Massachusetts, pp 291–308


Sharma RK, Sharma S, Sharma SS (2006) Seed germination behaviour of some medicinal plants of Lahaul and Spiti cold desert (Himachal Pradesh): Implications for conservation and cultivation. Curr Sci 90:1113–1118


Skvarla JJ, Raven PH, Praolowski J (1975) The evolution of pollen tetrads in Onagraceae. Am J Bot 62:6–35


Soley N (2013) Reproductive biology of the invasive plant Elaeagnus umbellata: breeding system, pollinators, and implications for invasive spread. Reprod Biol 5:1


Suzuki K, Takeda H, Tsukaguchi T, Egawa Y (2001) Ultra structural study on degeneration of tapetum in anther of snap bean (Phaseolus vulgaris L.) under heat stress. Sex Plant Reprod 13:293–299


Swanson SD, Sohmer SH (1976) The biology of Podophyllum peltatum L. (Berberidaceae), the May apple II. The transfer of pollen and success of sexual reproduction. Bull Torrey Bot Club 103:223–226. https://doi.org/10.2307/2484683





Thuiller W, Richardson DM, Rouget M, Procheş S, Wilson JR (2006) Interactions between environment, species traits, and human use describe patterns of plant invasions. Ecology 87:1755–1769


Vale M, Nguyen DE, Dupouey J (2005) Microbial activity in the rhizosphere soil of six herbaceous species cultivated in a greenhouse is correlated with shoot biomass and root C concentrations. Soil Biol Biochem 37:2329–2333. https://doi.org/10.1016/j.soilbio.2005.04.014


Wang Y, Chen S (2001) Pollen flora of Yuenyang lake Nature preserve Taiwan (II). Taiwania 46:167–191





Yamauchi Y, Ogawa N, Kuwahara A, Hanada A, Kamiya Y, Yamaguchi S (2004) Activation of gibberellin biosynthesis response pathways by low temperature during imbibitions of Arabidopsis thaliana seeds. Plant Cell 16:367–378

 


Acknowledgements


We are highly thankful to the Department of Botany, University of Kashmir for providing the necessary facilities.


Author Information


Ashraf Asma
Plant Reproductive Biology, Genetic Diversity and Phytochemistry Research Laboratory, Department of Botany, University of Kashmir, Srinagar, India

Hassan Afrozah
Plant Reproductive Biology, Genetic Diversity and Phytochemistry Research Laboratory, Department of Botany, University of Kashmir, Srinagar, India
malikaafreen6@gmail.com

Nawchoo Irshad A.
Plant Reproductive Biology, Genetic Diversity and Phytochemistry Research Laboratory, Department of Botany, University of Kashmir, Srinagar, India