Seed longevity and storage dependent changes in seed germination aspects of Berberis aristata and B. lycium from Western Himalaya: implications for seed storage and conservation

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00505-0
First Page: 1191
Last Page: 1198
Views: 1394


Keywords: Berberis aristata , B. lycium , Conservation, Endangered, Seed germination, Longevity


Abstract


Seed longevity is an important feature in the context of agriculture, biodiversity conservation, gene banks and plant adaptation to diverse environmental adversities. It varies extensively, from a few days to many years, in a species-specific manner. The present study was aimed at assessing the seed longevity under ambient storage conditions and characterizing the storage dependent changes in viability, germination and efficacy of certain seed pre-treatments in Berberis aristata Roxb. ex DC. and B. lycium Royle (Berberidaceae) from different altitudes in Kullu, Himachal Pradesh, India. Both the species have remarkably high medicinal and economic importance and are threatened. The freshly harvested seeds exhibited 100% viability and a reasonably good germination i.e., 53%, 60% and 77% in B. aristata (2400 m), B. lycium (2100 m) and B. lycium (1200 m), respectively, that was further enhanced to varying extents through certain seed pre-treatments. The seed viability status declined consistently with the progression of storage period; in B. aristata, 100% viability status was retained upto 6 months. Storage of seeds for 18 months under ambient conditions led to 33% and 100% viability loss in B. aristata (2400 m), and B. lycium (1200, 2100 m), respectively. A concomitant storage-dependent decline in seed germination was observed. The order of seed longevity (time taken for 50% decline in seed viability) was: B. aristata (2400 m) > 20 months, B. lycium (2100 m) > 10 months and B. lycium (1200 m) > 8 months. The stored seeds responded to various pretreatments in a generally comparable manner to that in case of the freshly harvested ones. The observations on diverse seed biological aspects have direct implications for seed storage, conservation and propagation of the species studied.



              Berberis aristata
            , 
              B. lycium
            , Conservation, Endangered, Seed germination, Longevity


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References


Anis KV, Rajeshkumar NV, Kuttan R (2001) Inhibition of chemical carcinogenesis by berberine in rats and mice. J Pharm Pharmacol 53(5):763–768


Ballesteros D, Pence VC (2017) Survival and death of seeds during liquid nitrogen storage: a case study on seeds with short life spans. CryoLetters 38(4):278–289


Ballesteros D, Pritchard HW, Walters C (2020) Dry architecture: towards the understanding of the variation of longevity in desiccation-tolerant germplasm. Seed Sci Res 30(2):142–155


Baskin J, Baskin CC (2004) A classification system for seed dormancy. Seed Sci Res 14(1):1–16


Belwal T, Bisht A, Bhatt ID, Rawal RS (2015) Influence of seed priming and storage time on germination and enzymatic activity of selected Berberis species. Plant Growth Regul 77:189–199


Bethke PC, Guber F, Jacobsen JV, Jones RL (2004) Dormancy of Arabidopsis seeds and barley grains can be broken by nitric oxide. Planta 219(5):847–855


Bewley JD, Black M (1982) Physiology and biochemistry of seeds, vol 2. Springer-Verlag, Berlin, Heidelberg


Brijwal L, Pandey A, Tamta S (2015) In vitro propagation of the endangered species Berberis aristata DC. via leaf-derived callus. In Vitro Cell Dev Biol Plant 51(6):637–647


Clerkx EJM, Blankestijn-De Vries H, Ruys GJ, Groot SPC, Koornneef M (2004) Genetic differences in seed longevity of various Arabidopsis mutants. Physiol Plant 121(3):448–461


Davies RM, Dickie JB, Ballesteros D (2018) Evaluation of short-lived seeds’ cryopreservation as alternative to conventional seed banking. Cryobiol 85:140–141


Daws MI, Davies J, Vaes E, Van Gelder R, Pritchard HW (2007) Two-hundred-year seed survival of Leucospermum and two other woody species from the Cape Floristic region, South Africa. Seed Sci Res 17(2):73–79


Ellis RH, Hong TD, Roberts EH (1990) Moisture content and the longevity of seeds of Phaseolus vulgaris. Ann Bot 66:341–348


Groot SPC, Karssen CM (1987) Gibberellins regulate seed germination in tomato by endosperm weakening: a study with gibberellin-deficient mutants. Planta 171:525–531


Guo FQ, Mamoru O, Crawford NM (2003) Identification of a plant nitric oxide synthase gene involved in hormonal signaling. Science 302:100–103


Hartmann HT, Kester DE, Davies ET (1989) Plant propagation: principles and practices. Prantice Hall, USA


Hayat S, Hayat Q, Nasser M, Alyemeni MN, Wani AS, Pichtel J, Ahmad A (2012) Role of proline under changing environments. Plant Signal Behav 7(11):1456–1466. DOI: https://doi.org/10.4161/psb.21949


Hendricks SB, Taylorson RB (1974) Promotion of seed germination by nitrate, nitrite, hydroxylamine, and ammonium salts. Plant Physiol 54(3):304–309


Janbaz KH, Gilani AH (2000) Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia 71(1):25–33


Jin Y (2011) Role of Berberis spp. as alternate hosts in generating new races of Puccinia graminis and P. striiformis. Euphytica 179:105–108. https://doi.org/10.1007/s10681-010-0328-3


Kala CP (2002) Indigenous knowledge of Bhotiya tribal community on wool dyeing and its present status in the Garhwal Himalaya, India. Curr Sci 83(7):814–817


Kaul S, Sharma SS, Mehta IK (2008) Free radical scavenging potential of L-proline: evidence from in vitro assays. Amino Acids 34:315–320


Kim DH (2018) Extending Populus seed longevity by controlling seed moisture content and temperature. PLoS ONE 13(8):e0203080. https://doi.org/10.1371/journal.pone.0203080


Leino MW, Edqvist J (2010) Germination of 151-year-old Acacia spp. seeds. Genet Resour Crop Evol 57(5):741–746. https://doi.org/10.1007/s10722-009-9512-5


Leprince O, Pellizzaro A, Berriri S, Buitink J (2017) Late seed maturation: drying without dying. J Exp Bot 68(4):827841


Maroder HL, Prego IA, Facciuto GR, Maldonado SB (2000) Storage behavior of Salix alba and Salix matsudana seeds. Ann Bot 86(5):1017–1021


McIntyre GI, Cessna AJ, Hsiao AI (1996) Seed dormancy in Avena fatua: Interacting effects of nitrate, water, and seed coat injury. Physiol Plant 97(6):291–302


Milberg P (1994) Germination of up to 129-year-old, dry stored seeds of Geranium bohemicum (Geraniaceae). Nordic J Bot 14(1):27–29


Miura K, Lin Y, Yano M, Nagamine T (2002) Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.). Theor Appl Genet 104:981–986. https://doi.org/10.1007/s00122-002-0872-x


Murthy UN, Sun WQ (2000) Protein modification by Amadori and Maillard reactions during seed storage: roles of sugar hydrolysis and lipid peroxidation. J Exp Bot 51(348):1221–1228


Nagel M, Seal CE, Colville L, Rodenstein A, Un S et al (2019) Wheat seed ageing viewed through the cellular redox environment and changes in pH. Free Rad Res 53(6):641–654


Nazir N, Rahman A, Uddin F, Khan Khalil AA, Zahoor M et al (2021) Quantitative ethnomedicinal status and phytochemical analysis of Berberis lycium Royle. Agronomy 11(1):130. https://doi.org/10.3390/agronomy11010130


Neill SJ, Desikan R, Hancock JT (2003) Nitric oxide signalling in plants. New Phytol 159(1):11–35


Parmar C, Khamu V (1989) A note on the sexual and asexual propagation of the Indian barberry (Berberis aristata DC). Indian For 115(7):508–509


Rajjou L, Lovigny Y, Groot SPC, Belghazi M, Job C et al (2008) Proteome-wide characterization of seed aging in Arabidopsis: a comparison between artificial and natural aging protocols. Plant Physiol 148(1):620–641


Salehi B, Selamoglu Z, Sener B et al (2019) Berberis plants-drifting from farm to food applications, phytotherapy, and phytopharmacology. Foods 8(10):522. https://doi.org/10.3390/foods8100522


Sallon S, Solowey E, Cohen Y, Korchinsky R, Egli M et al (2008) Germination, genetics, and growth of an ancient date seed. Science 320(5882):1464. DOI: https://doi.org/10.1126/science.1153600


Sano N, Rajjou L, North HM, Debeaujon I, Poll AM et al (2016) Staying alive: molecular aspects of seed longevity. Plant Cell Physiol 57(4):660–674


Schwember AR, Bradford KJ (2010) Quantitative trait loci associated with longevity of lettuce seeds under conventional and controlled deterioration storage conditions. J Exp Bot 61(15):4423–4436


Sharma RK (2021) Physical seed dormancy in Abrus precatorious (Ratti): a scientific validation of indigenous technique. Exp Results 2:e8. https://doi.org/10.1017/exp.2020.64


Sharma SS, Dietz KJ (2006) The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. J Exp Bot 57(4):711–726


Sharma S, Sharma RK (2010b) Seed physiological aspects of Pushkarmool (Inula racemosa), a threatened medicinal herb: response to storage, cold-stratification, light and gibberellic acid. Curr Sci 99(12):1801–1806


Sharma RS, Mishra V, Singh R, Seth N, Babu CR (2008) Antifungal activity of some Himalayan medicinal plants and cultivated ornamental species. Fitoterapia 79(7–8):589–591


Sharma RK, Sharma S, Sharma SS (2010) Storage-dependent changes in dormancy and germination of Himalayan Mayapple (Podophyllum hexandrum Royle) seeds and their response to gibberellic acid. J Herbs Spices Med Plants 16(1):1–14


Sharma RK, Sharma S, Sharma SS (2014) The long-term ambient storage-induced alterations in seed viability, germination and seedling vigour of Saussurea costus, a critically endangered medicinal herb of Northwest Himalaya. J App Res Med Arom Plants 1(3):92–97


Sharma A, Sharma RK, Sharma SS (2021) A comparable stimulation of seed germination by gibberellic acid and sodium nitroprusside in Hyoscyamus niger L., a threatened medicinal herb, from Spiti (Himachal Pradesh). J Ind Bot Soc 101(3):194–203


Shen-Miller J (2002) Sacred lotus, the long-living fruits of China Antique. Seed Sci Res 12(3):131–143


Shetty K (2004) Role of proline-linked pentose phosphate pathway in biosynthesis of plant phenolics for functional food and environmental applications: a review. Process Biochem 39:789–804. https://doi.org/10.1016/S0032-9592(03)00088-8


Singh J, Kakkar P (2009) Antihyperglycemic and antioxidant effect of Berberis aristata root extract and its role in regulating carbohydrate metabolism in diabetic rats. J Ethnopharmacol 123(1):22–26


Sood H, Kumar Y, Gupta VK, Arora DS (2019) Scientific validation of the antimicrobial and antiproliferative potential of Berberis aristata DC root bark, its phytoconstituents and their biosafety. AMB Express 9:143–158


Steiner AM, Ruckenbauer P (1995) Germination of 110-yearold cereal, weed seeds, the Vienna sample of 1877. Verification of effective ultra-dry storage at ambient temperature. Seed Sci Res 5(4):195–199


Sun C, Zhang Y, Liu L et al (2021) Molecular functions of nitric oxide and its potential applications in horticultural crops. Hort Res 8:71. https://doi.org/10.1038/s41438-021-00500-7


Thakur AS, Thakur PS, Mehta R (2005) Effect of pre-sowing treatments on seed germination in Berberis aristata. Indian J Plant Physiol 10(4):338–343


Tiwari UL, Adhikari BS, Rawat GS (2012) A checklist of Berberidaceae in Uttarakhand, Western Himalaya, India. CheckList 8(4):610–616


Wang Y, Li Y, Xue H, Pritchard HW, Wang XF (2015) Reactive oxygen species (ROS)-provoked mitochondria-dependent cell death during ageing of elm (Ulmus pumila L.) seeds. Plant J 81(3):438–452


Yan A, Chen Z (2020) The control of seed dormancy and germination by temperature, light and nitrate. Bot Rev 86:39–75


Zhang H, Shen WB, Xu LL (2003) Effect of nitric oxide on the germination of wheat seed and its reactive oxygen species metabolisms under osmotic stress. Acta Bot Sin 45(8):901–905


Ziegler V, Paraginski RT, Ferreira CD (2021) Grain storage systems and effects of moisture, temperature, and time on grain quality-a review. J Stored Prod Res 91:1–11


CAMP Report (2010) Conservation Assessment & Management Prioritisation (CAMP). Workshop on threatened medicinal plants of Himachal Pradesh. Sponsored by National Medicinal Plants Board, Government of India and organised by Himachal Pradesh Forest Department, Shimla


Ludwig V, Berghetti MRP, Rossato FP, Wendt LM, Schultz EE, Both V, Brackmann A (2021) Impact of controlled atmosphere storage on physiological quality of soybean seed. J Stored Prod Res 90. https://doi.org/10.1016/j.jspr.2020.101749


Malhotra B, Kulkarni GT, Dhiman N, Joshi DD, Chander S et al (2021) Recent advances on Berberis aristata emphasizing berberine alkaloid including phytochemistry, pharmacology and drug delivery system. J Herb Med 27. https://doi.org/10.1016/j.hermed.2021.100433


Sharma S, Sharma RK (2010a) Long-term storage-dependent changes in seed physiological aspects of Hyoscyamus niger from a cold desert region of Trans-Himalaya. Int J Plant Biol 1(2), e17:84–88

 


Acknowledgements


Financial assistance as JRF and SRF to Ram Krishan by Indian Council of Medical Research (ICMR), New Delhi (3/1/3/JRF-2014/HRD-078/10023) is thankfully acknowledged.


Author Information


Krishan Ram
Department of Biosciences, Himachal Pradesh University, Shimla, India

Sharma Rajender Kumar
Department of Botany, Deshbandhu College, University of Delhi, Delhi, India


Kumar Vijay
Department of Biosciences, Himachal Pradesh University, Shimla, India