Vegetos

Abstract

Cleome spinosa Jacq. (Family Cleomaceae) is an ornamental plant having horizontal racemes of white to pink flowers, 5–7 digitate, alternate leaves, numerous brown seeds in siliqua, attaining height of 1–1.5 m. Cleome gyandra Linn. another plant of this family is annual, erect, herbaceous weed growing on waste lands having 3–5 foliate leaves. Literature reveals both plants having active ingredients, possessing medicinal properties.

Molecular characterization by RAPD markers is more reliable because the genetic information is unique and can be employed for different species of same genus. In present investigation the genomic DNA of Cleome spinosa Jacq and Cleome gynandra Linn. was isolated by CTAB methodology, purified and estimated for purity and concentration using qualitative and quantitative measures. As PCR based markers depend on purity of DNA, the extracted DNA was repeatedly purified. The quantity of DNA extracted from Cleome spinosa Jacq. and Cleome gynandra Linn. leaves were found to be 34μg/mL and 22μg/mL respectively. Purified DNA was subjected to PCR amplification and RAPD analysis.