Sub-lethal effect of diesel fuel on the development of in vitro produced callus culture of Tagetes patula L. (Marigold-Nemo mix)

,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00390-7
First Page: 1118
Last Page: 1127
Views: 100


Keywords: Diesel-treated calli, Plant preservative mixture (PPM), Plant growth regulators, Polycyclic aromatic hydrocarbon (PAH)


Abstract


Plant tissue culture has been applied to study different abiotic stress agents that were included in the culture medium for treatment of callus. Here, the requirements for high efficiency (about 95%) of callus initiation in leaf explants of Tagetes patula (marigold) were first established. Green calli were formed on Murashige and Skoog medium supplemented with 1.8 µM NAA and 66.6 µM BA. There was no prior study on the effect of diesel on T. patula callus, probably because diesel cannot be applied to plant tissue culture medium or used safely in the culture environment. These technical challenges were overcame here using a new protocol for exposure of T. patula calli to diesel fuel under non-aseptic conditions whereas earlier the diesel-treated calli were cultured under aseptic conditions. Immediately after up to 9 min of diesel exposure, the T. patula calli did not showed any sign of necrosis. Non-necrotic, proliferating T. patula calli were subsequently obtained on a callus induction medium supplemented with 1.8 µM NAA and 66.6 µM BA. In a histological study, the internal organisation of the diesel-treated T. patula calli were different from the control calli. In the transverse sections at 500 µm taken from the surface of the control calli, there were more cells with lignified cell walls and xylem vessels than the diesel-treated calli. Well-formed shoot apical meristem domes with leaf primordia were found in the transverse sections at 1500 µm from the surface of the control callus but were absent in the diesel-treated calli. This result suggests that the innovative protocol of diesel exposure was sub-lethal, and after further investigation might enable the extension for the application of in vitro elicitation technique to produce callus culture or shoots with the desired amount of secondary metabolite.


Diesel-treated calli, Plant preservative mixture (PPM), Plant growth regulators, Polycyclic aromatic hydrocarbon (PAH)


*Pdf Download Buy Printed Copy

(*Only SPR Members can download pdf file; #Open Access;)

References


Aina R, Palin L, Citterio S (2006) Molecular evidence for benzo [a] pyrene and naphthalene genotoxicity in Trifolium repens L. Chemosphere 65(4):666–673


Al-Baldawi IA, Abdullah SRS, Anuar N, Suja F, Mushrifah I (2015) Phytodegradation of total petroleum hydrocarbon (TPH) in diesel-contaminated water using Scirpus grossus. Ecol Eng 74:463–473


Andrea B, Tani C (2009) Ultrastructural effects of salinity in Nicotiana bigelovii var. bigelovii callus cells and Allium cepa roots. Caryologia 62(2):124–133


Ashrafzadeh S, Leung DMW (2015) In vitro breeding of heavy metal-resistant plants: a review. Hortic Environ Biotechnol 56(2):131–136


Banerjee AK, Prat S, Hannapel DJ (2006) Efficient production of transgenic potato (S. tuberosum L. ssp. andigena) plants via Agrobacterium tumefaciens-mediated transformation. Plant Sci 170(4):732–738


Banno H, Ikeda Y, Niu Q-W, Chua N-H (2001) Overexpression of ArabidopsisESR1 induces initiation of shoot regeneration. Plant Cell 13(12):2609–2618


Barros J, Serk H, Granlund I, Pesquet E (2015) The cell biology of lignification in higher plants. Ann Bot 115(7):1053–1074


Benítez-García I, Vanegas-Espinoza PE, Meléndez-Martínez AJ, Heredia FJ, Paredes-López O, Villar-Martínez D, Angélica A (2014) Callus culture development of two varieties of Tagetes erecta and carotenoid production. Electron J Biotechnol 17(3):107–113


Bennici A, Tani C (2012) Ultrastructural characteristics of callus cells of Nicotiana tabacum L. var. BELW3 grown in presence of NaCl. Caryologia 65(1):72–81


Bünger J, Krahl J, Munack A, Ruschel Y, Schröder O, Emmert B et al (2007) Strong mutagenic effects of diesel engine emissions using vegetable oil as fuel. Arch Toxicol 81(8):599–603


Bychkova OV, Khlebova LP, Plyushcheva ES, Borsukova AI (2018) Efficient induction of hairy roots in Tagetes patula L. Ukrain J Ecol 8(4):450–453


Chakraborty N, Banerjee D, Ghosh M, Pradhan P, Gupta NS, Acharya K, Banerjee M (2013) Influence of plant growth regulators on callus mediated regeneration and secondary metabolites synthesis in Withania somnifera (L.) Dunal. Physiol Mol Biol Plants 19(1):117–125


Chen L, Liu X, Zhang X, Liu S, Wei J, Xu G (2013) Response characteristics of seed germination and seedling growth of Acorus tatarinowii under diesel stress. Plant Soil 368(1–2):355–363


Colón-Carmona A, You R, Haimovitch-Gal T, Doerner P (1999) Spatio-temporal analysis of mitotic activity with a labile cyclin–GUS fusion protein. Plant J 20(4):503–508


Croes AF, Van den Berg AJR, Bosveld M, Breteler H, Wullems GJ (1989) Thiophene accumulation in relation to morphology in roots of Tagetes patula. Planta 179(1):43–50


Cusido RM, Onrubia M, Sabater-Jara AB, Moyano E, Bonfill M, Goossens A et al (2014) A rational approach to improving the biotechnological production of taxanes in plant cell cultures of Taxus spp. Biotechnol Adv 32(6):1157–1167


Doerner P, Jørgensen J-E, You R, Steppuhn J, Lamb C (1996) Control of root growth and development by cyclin expression. Nature 380(6574):520


Felek W, Mekibib F, Admassu B (2015) Optimization of explants surface sterilization condition for field grown peach (Prunus persica L. Batsch. Cv. Garnem) intended for in vitro culture. Afr J Biotechnol 14(8):657–660


Gavnholt B, Larsen K (2002) Molecular biology of plant laccases in relation to lignin formation. Physiol Plant 116(3):273–280


Ghanati F, Morita A, Yokota H (2005) Effects of aluminum on the growth of tea plant and activation of antioxidant system. Plant Soil 276(1–2):133–141


Gupta V, Shanker K, ur Rahman L (2016) In vitro production of thiophenes using hairy root cultures of Tagetes erecta (L.). Afr J Biotechnol 15(17):706–713


Hong E, Kang H (2011) Effect of smoke and aspirin stimuli on the germination and growth of alfalfa and broccoli. Electron J Environ Agric Food Chem 10(2):1918–1926


Huang J, Gu M, Lai Z, Fan B, Shi K, Zhou Y-H et al (2010) Functional analysis of the Arabidopsis PAL gene family in plant growth, development, and response to environmental stress. Plant Physiol 153(4):1526–1538


Jain R, Katare N, Kumar V, Kumar A, Goswami SS, Shrotri CK (2012) In vitro antibacterial potential of different extracts of Tagetes erecta and Tagetes patula. J Nat Sci Res 2(5):84–91


Johansen DA (1940) Plant microtechique. McGraw-Hill Book Company, Inc., London


Jouannic S, Lartaud M, Hervé J, Collin M, Orieux Y, Verdeil J-L, Tregear JW (2011) The shoot apical meristem of oil palm (Elaeis guineensis; Arecaceae): developmental progression and dynamics. Ann Bot 108(8):1477–1487


Karjee S, Namita KPS, Panwar S (2020) Development of in-vitro protocol for direct regeneration from thalamus ex-plant of Tagetes patula L. var. Pusa Deep. J Pharmacognd Phytochem 9(4):373–377


Kärkönen A, Koutaniemi S (2010) Lignin biosynthesis studies in plant tissue cultures. J Integr Plant Biol 52(2):176–185


Kasperbauer MJ, Eizenga GC (1985) Tall fescue doubled haploids via tissue culture and plant regeneration 1. Crop Sci 25(6):1091–1095


Kedra M, Bach A (2005) Morphogenesis of Lilium martagon L. explants in callus culture. Acta Biol Crac Ser Bot 47(1):65–73


Ketel DH (1986) Morphological differentiation and occurrence of thiophenes in leaf callus cultures from Tagetes species: relation to the growth medium of the plants. Physiol Plant 66(3):392–396


Ketel DH (1987) Distribution and accumulation of thiophenes in plants and calli of different Tagetes species. J Exp Bot 38(2):322–330


Ketel DH, Breteler H, De Groot B (1985) Effect of explant origin on growth and differentiation of calli from Tagetes species. J Plant Physiol 118(4):327–333


Kumar KR, Singh KP, Bhatia R, Raju DVS, Panwar S (2019) Optimising protocol for successful development of haploids in marigold (Tagetes spp.) through in vitro androgenesis. Plant Cell Tissue Organ Cult (PCTOC) 138(1):11–28


Lapuerta M, Rodríguez-Fernández J, Patiño-Camino R, Cova-Bonillo A, Monedero E, Meziani YM (2020) Determination of optical and dielectric properties of blends of alcohol with diesel and biodiesel fuels from terahertz spectroscopy. Fuel 274:117877


Lewtas J (1983) Evaluation of the mutagenicity and carcinogenicity of motor vehicle emissions in short-term bioassays. Environ Health Perspect 47:141–152


Liang Y, Siddaramu T, Yesuf J, Sarkany N (2010) Fermentable sugar is release from Jatropha seed cakes following lime pretreatment and enzymatic hydrolysis. Bioresour Technol 101(16):6417–6424


Lima JD, Mazzafera P, Moraes WDS, Silva RBD (2009) Chá: aspectos relacionados à qualidade e perspectivas. Ciência Rural 39:1270–1278


Ling APK, Tan KP, Hussein S (2013) Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata. J Zhejiang Univ Sci B 14(7):621–631


Liu Q, Zheng L, He F, Zhao F-J, Shen Z, Zheng L (2015) Transcriptional and physiological analyses identify a regulatory role for hydrogen peroxide in the lignin biosynthesis of copper-stressed rice roots. Plant Soil 387(1–2):323–336


Mintoff SJL, Rookes JE, Cahill DM (2015) Sub-lethal UV-C radiation induces callose, hydrogen peroxide and defence-related gene expression in Arabidopsis thaliana. Plant Biol 17(3):703–711


Mohamed M-H, Harris PJC, Henderson J (1998) An efficient in vitro regeneration protocol for Tagetes minuta. Plant Cell Tissue Organ Cult 55(3):211–215


Mori S, Adachi Y, Horimoto S, Suzuki S, Nakano M (2005) Callus formation and plant regeneration in various Lilium species and cultivars. In Vitro Cell Dev Biol Plant 41(6):783–788


Moura JCMS, Bonine CAV, de Oliveira Fernandes Viana J, Dornelas MC, Mazzafera P (2010) Abiotic and biotic stresses and changes in the lignin content and composition in plants. J Integr Plant Biol 52(4):360–376


Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15(3):473–497


Nagella P, Murthy HN (2010) Establishment of cell suspension cultures of Withania somnifera for the production of with anolide A. Bioresour Technol 101(17):6735–6739


Qi Y, Ye Y, Bao M (2011) Establishment of plant regeneration system from anther culture of Tagetes patula. Afr J Biotechnol 10(75):17332–17338





Rao AQ, Hussain SS, Shahzad MS, Bokhari S, Raza MH, Rakha A et al (2006) Somatic embryogenesis in wild relatives of cotton (Gossypium spp.). J Zhejiang Univ Sci B 7(4):291–298


Ruzin SE (1999) Plant microtechnique and microscopy, vol 198. Oxford University Press, New York


Singh P, Krishna A, Kumar V, Krishna S, Singh K, Gupta M, Singh S (2016) Chemistry and biology of industrial crop Tagetes species: a review. J Essent Oil Res 28(1):1–14


Stewart C, Giannini J (2016) Inexpensive, open-source epifluorescence microscopes. J Chem Educ 93:1310–1315


Teixeira da Silva JA (2012) Callus, calluses or calli: multiple plurals. Asian Australas J Plant Sci Biotechnol 6(1):125–126


US Department of Health and Human Services (1995) Agency for Toxic Substances and Disease Registry-ATSDR. Toxicological profile for fuel oils. Department of Health and Human Services, Public Health Service, Atlanta, GA. https://www.atsdr.cdc.gov/phs/phs.asp?id=514&tid=91


Vengadesan G, Pijut PM (2009) Somatic embryogenesis and plant regeneration of northern red oak (Quercus rubra L.). Plant Cell Tissue Organ Cult (PCTOC) 97(2):141–149


Wang X, Yu X, Bartha R (1990) Effect of bioremediation on polycyclic aromatic hydrocarbon residues in soil. Environ Sci Technol 24(7):1086–1089


Wang XD, Nolan KE, Irwanto RR, Sheahan MB, Rose RJ (2011) Ontogeny of embryogenic callus in Medicago truncatula: the fate of the pluripotent and totipotent stem cells. Ann Bot 107(4):599–609


Wante SP, Leung DWM (2018) Phytotoxicity testing of diesel-contaminated water using Petunia grandiflora Juss. Mix F1 and Marigold-Nemo Mix (Tagetes patula L.). Environ Monit Assess 190(7):408


Zagoskina NV, Dubravina GA, Alyavina AK, Goncharuk EA (2003) Effect of ultraviolet (UV-B) radiation on the formation and localization of phenolic compounds in tea plant callus cultures. Russ J Plant Physiol 50(2):270–275

 


Acknowledgements


S. P. Wante was supported by a Tertiary Education Trust Fund (TETFund) scholarship, Nigeria to carry out a PhD study at the University of Canterbury, New Zealand.


Author Information


Wante Solomon Peter
Department of Biological Sciences, Federal University of Kashere, Gombe, Nigeria
solomonpeterwante@yahoo.com
Leung David W. M.
Department of Biological Sciences, Federal University of Kashere, Gombe, Nigeria