Vegetos
(An International Journal of Plant Research & Biotechnology)
The biofabrication of silver nanoparticles from Artemisia maritima Linn. and the analysis of their bioactive properties
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Research Articles | Published: 27 May, 2025
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Keywords: Himalaya, GCMS, Silver-nanoparticles, Antioxidant, Disk-diffusion, MIC
Abstract
The presented research work encompasses outcomes from different methods performed to assess the bioactive properties of aerial parts of Artemisia maritima and the biogenic synthesis of silver nanoparticles. Bioactive properties of plant parts were screened in terms of phytochemical components (total phenols, flavonoids, tannin contents (TPC, TFC, and TTC), and GCMS analysis), enzyme inhibitory potential (α-amylase), FTIR (Fourier Transform Infrared), antioxidant, and antimicrobial activities. Biofabricated silver nanoparticles were characterized by UV-Vis spectroscopy, FTIR, XRD, FESEM, EDX, and HRTEM, their antioxidant and antimicrobial potentials were also assessed. Plant material after extraction demonstrated a higher percentage yield in methanol solvent than in acetone. Both extracts revealed the presence of phenols, flavonoids, and tannins. However, between both extracts, the acetone extract revealed a higher TPC and TTC. On the other hand, methanol extract disclosed higher TFC. GCMS analysis indicated α-santonin, 1,3,6,10-cyclotetradecatetraene, 3,7,11-trimethyl-14-(1-methylethyl)-, [S-(E, Z,E, E)] and tetrapentacontane as major compounds with the highest percentage area in both solvents. Plant extracts also reported α-amylase inhibition potential with IC50 values of 5.67 mg/mL (acetone) and 13.32 mg/mL (methanol). After characterization, the obtained nanoparticles displayed the presence of different functional groups (FTIR), almost spherical shapes (FESEM, HRTEM), and crystalline nature (XRD, SAED). Nanoparticles also exhibited antioxidant and antimicrobial activities, along with plant extracts.
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Author Information
Department of Biosciences, Himachal Pradesh University, Summer Hill, Shimla, India