Keywords: Chromosomes aberration, Chemical doubling agent, Growth parameters, Meiosis, Pollen‐fertility, Polyploidy
We have prepared various colchicine concentrations and applied them on seedling of Chrysanthemum carinatum (2n = 18) and seeds of Vicia faba (2n = 12). Colchicine-induced polyploidy was reported in Chrysanthemum carinatum (2n = 4x = 36) and Vicia faba (2n = 4x = 24) plants. This research explains the polyploidy in respective plants which successfully results in apparent morphological and cytological changes in polyploid plants compared to the control plants. Phenotypic characters exhibited that polyploid plants initially had slightly slower growth, stronger stems, thicker and larger leaves, larger flowers, and seeds and then increases abruptly. There was an increment in cytological characters such as cell size, chiasma frequency in polyploid plants as compared to the control plants. Growth patterns, morphology, pollen staining, and seed sets were recorded in this work. However, the main emphasis was laid on the behavior of chromosomes of isolated polyploids.
The authors are grateful to the Head of the Department, School of Studies in Botany, Jiwaji University, Gwalior for providing support.