Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Untargeted GC–MS profiling of compounds in Taxus wallichiana Zucc. bark and needles from western Himalaya
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Research Articles | Published: 15 June, 2026
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Keywords: Metabolite profiling, GC–MS, n Taxus wallichianan
Abstract
Taxus wallichiana Zucc. is a medicinally important plant valued in ethnomedicine and pharmacology for its diverse phytochemical repertoire and therapeutic potential, particularly in cancer treatment. In this study, untargeted gas chromatography–mass spectrometry (GC–MS) was employed to comprehensively profile the metabolites present in the bark and needles of T. wallichiana. Sequential extraction with n-hexane and ethyl acetate enabled comparative analysis of metabolite classes across tissues. Distinct chemical compounds were observed: needles were enriched in phenolics, alcohols, and fatty acids, whereas bark extracts contained higher proportions of hydrocarbons, alkanes, and heterocyclic compounds. These results highlight tissue- and solvent-dependent variations in metabolite distribution and expand current understanding of the phytochemical diversity of T. wallichiana. Such insights not only reinforce its significance in traditional medicine but also strengthen its potential as a reservoir of bioactive molecules for modern drug discovery.
References
Adhikari P, Joshi K, Singh M, Pandey A (2020) Influence of altitude on secondary metabolites, antioxidants, and antimicrobial activities of Himalayan yew (Taxus wallichiana). Plant Biosys 0:000–000. https://doi.org/10.1080/11263504.2020.1845845
de Alencar MVOB, Islam MT, de Lima RMT, Paz MFCJ, Dos Reis AC, da Mata AMOF et al (2019) Phytol as an anticarcinogenic and antitumoral agent: an in vivo study in Swiss mice with DMBA-induced breast cancer. IUBMB Life 71:200–212. https://doi.org/10.1002/iub.1952
Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M (2012) Anti-inflammatory property of n-hexadecanoic acid: structural evidence and kinetic assessment. Chem Biol Drug des 80:434–439. https://doi.org/10.1111/j.1747-0285.2012.01418.x
Chattopadhyay SK, Pal A, Maulik PR, Kaur T, Garg A, Khanuja SPS (2006) Taxoid from the needles of the Himalayan yew Taxus wallichiana with cytotoxic and immunomodulatory activities. Bioorg Med Chem Lett 16:2446–2449. https://doi.org/10.1016/j.bmcl.2006.01.077
Dang PH, Nguyen HX, Duong TTT, Tran TKT, Nguyen PT, Vu TKT et al (2017) α-Glucosidase inhibitory and cytotoxic taxane diterpenoids from the stem bark of Taxus wallichiana. J Nat Prod 80:1087–1095. https://doi.org/10.1021/acs.jnatprod.7b00006
Ganesan T, Subban M, Christopher Leslee DB, Kuppannan SB, Seedevi P (2022) Structural characterization of n-hexadecanoic acid from the leaves of Ipomoea eriocarpa and its antioxidant and antibacterial activities. Biomass Convers Biorefin 12:1–12. https://doi.org/10.1007/s13399-022-03576-w
Kaari M, Joseph J, Manikkam R, Kalyanasundaram R, Sivaraj A, Anbalmani S et al (2023) A novel finding: 2,4-di-tert-butylphenol from Streptomyces bacillaris ANS2 effective against Mycobacterium tuberculosis and cancer cell lines. Appl Biochem Biotechnol 195:1–14. https://doi.org/10.1007/s12010-023-04403-2
Khan I, Nisar M, Zarrelli A, Di Fabio G, Gul F, Gilani SN et al (2013) Molecular insights to explore abietane diterpenes as new LOX inhibitors. Med Chem Res 22:5809–5813. https://doi.org/10.1007/s00044-013-0559-7
Khan M, Verma SC, Srivastava SK, Shawl AS, Syamsundar KV, Khanuja SPS et al (2006) Essential oil composition of Taxus wallichiana Zucc. from the northern Himalayan region of India. Flavour Fragr J 21:772–775. https://doi.org/10.1002/ffj.1682
Nisar M, Khan I, Ahmad B, Ali I, Ahmad W, Choudhary MI (2008a) Antifungal and antibacterial activities of Taxus wallichiana Zucc. J Enzyme Inhib Med Chem 23:256–260. https://doi.org/10.1080/14756360701505336
Nisar M, Khan I, Simjee SU, Gilani AH, Obaidullah PH (2008b) Anticonvulsant, analgesic and antipyretic activities of Taxus wallichiana Zucc. J Ethnopharm 116:490–494. https://doi.org/10.1016/j.jep.2007.12.021
Adhikari P, Pandey A, Agnihotri V, Pande V (2018) Selection of solvent and extraction method for determination of antimicrobial potential of Taxus wallichiana Zucc. Res Pharm Sci 8:1–9. https://doi.org/10.25081/rip.2018.v8.3487
Sansenya S, Payaka A, Mansalai P (2023) Biological activity and inhibition potential against α-glucosidase and α-amylase of 2,4-di-tert-butylphenol from bamboo shoot extract by in vitro and in silico studies. Process Biochem 126:15–22. https://doi.org/10.1016/j.procbio.2022.12.033
Shariare MH, Noor HB, Khan JH, Uddin J, Ahamad SR, Altamimi MA et al (2021) Liposomal drug delivery of Corchorus olitorius leaf extract containing phytol using design of experiment (DoE): in vitro anticancer and in vivo anti-inflammatory studies. Colloids Surf B Biointerfaces 199:111543. https://doi.org/10.1016/j.colsurfb.2020.111543
Uddin SJ, Grice D, Tiralongo E (2012) Evaluation of cytotoxic activity of patriscabratine, tetracosane and various flavonoids isolated from the Bangladeshi medicinal plant Acrostichum aureum. Pharm Bio 50:1276–1280. https://doi.org/10.3109/13880209.2012.673628
Yousaf A, Waseem M, Javed A, Baig S, Ismail B, Baig A et al (2022) Augmented anticancer effect and antibacterial activity of silver nanoparticles synthesized using Taxus wallichiana leaf extract. PeerJ 10:e14391. https://doi.org/10.7717/peerj.14391
Author Information
University Centre for Research and Development, Chandigarh University, Mohali, India