Usage of biochar to ameliorate the toxicity induced by antibiotics for seedlings at the germination stage


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00395-2
First Page: 1096
Last Page: 1103
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Keywords: Soil amendment, Biochar, Pharmaceuticals, Tetracycline, Sulfamethazine, Plant germination, Growth parameters


Rice husk biochar (RB) was applied to the antibiotic-contaminated soil in a ratio of 5% (w/w) to investigate its ability to ameliorate the toxicity induced by antibiotics for seedlings at the germination stage. Aqueous solutions of tetracycline (TC) and sulfamethazine (SMT) with concentrations of 0, 1, 10, 20, 50 and 100 mg L−1 were applied to the soils (with/without RB). Plastic Petri dishes containing 75 g of the tested soil were sown by 30 seeds of each of tomato, lettuce, and Chinese cabbage. Petri dishes were incubated in the growth chamber for 7 days at 24 ± 2 °C, 80% of humidity and with 16 h light/8 h dark rotation. After 7 days, measurements of the germination rate and growth parameters of seedlings were recorded. Results revealed that the addition of RB to the soil decreased the toxicity effect of TC and SMT to the plants. Where, the RB/soil mixture maintained the germination rates over 80% and inhanced the total dry weight, shoot length, and root length of seedlings at levels of 0, 1, 10, and 20 mg L−1 of TC and SMT compared to the non-RB/soil. Therefore, RB can be applied to the soil as a fertilizer and as adsorbent material for the organic pollutants like antibiotics.

Soil amendment, Biochar, Pharmaceuticals, Tetracycline, Sulfamethazine, Plant germination, Growth parameters

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Author Information

Vu Ngoc-Thang
Faculty of Agronomy, Vietnam National University of Agriculture, Hanoi, Vietnam

Ahmed Mohamed Bedair M.
Department of Food Toxicology and Contaminants, National Research Centre, Cairo, Egypt