*Article not assigned to an issue yet
Khachtib Youssef, Bouda Said, Ait Bella Youssef, Zinelabidine Lalla Hasna, Haddioui Abdelmajid
Keywords:
Apple trees, Genetic diversity, ISSR markers, Polymorphism
Genetic diversity of 29 apple (Malus domestica) cultivars collected from different regions in Morocco was investigated using inter-simple sequence repeat (ISSR) markers. 15 ISSR primers generated 177 bands of which 156 are polymorphic (86.50%). The mean value of PIC, Rp, I and H were 0.46, 4.58, 0.43 and 0.28, respectively, implying important genetic variability between the studied apple cultivars. Furthermore, the coefficient of genetic differentiation among all groups was 0.27, indicating that 27% of total genetic variability was between groups and 73% was within groups. Bayesian analysis grouped the studied cultivars into three major clusters that are not in agreement, in major part, with their geographic origin and did not show any difference between local and introduced cultivars. Also the problem of synonyms, homonyms, and/or labeling errors appears in the studied cultivars. These results showed that the ISSR markers can be a useful tool for detecting the genetic diversity in this fruit crop.
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Ait Bella Y, Bouda S, Khachtib Y, Haddioui A (2021) Genetic variability of cultivated plum (Prunus domestica L. and Prunus salicina Lindl.) In Morocco assessed by ISSR markers. Aust J Crop Sci 15(6):948–954
Ajal EA, Jbir R, Legua P, Martínez JJ, Martinez R, Salhi-Hannachi A, Haddioui A (2015) Genetic diversity of moroccan pomegranate (Punica granatum L.) cultivars using AFLP markers. Aust J Crop Sci 9(1):22–29
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Earl DA, vonHoldt BM (2012) Structure Harvester: a website and program for visualizing structure output and implementing the Evanno method. Conserv Genet Resour 4:359–361. https://doi.org/10.1007/s12686-011-9548-7
Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x
FAO (2019) FAOSTAT. Food and Agriculture Organization of the United Nations, Rome, Italy. http://fao.org/faostat (accessed August 13, 2021)
Ferreira V, Ramos-Cabrer AM, Carnide V, Pinto-Carnide O, Assunção A, Marreiros A, Rodrigues R, Pereira-Lorenzo S, Castro I (2016) Genetic pool structure of local apple cultivars from Portugal assessed by microsatellites. Tree Genet Genomes 12(3):36. https://doi.org/10.1007/s11295-016-0997-8
Gasi F, Simon S, Pojskic N, Kurtovic M, Pejic I (2010) Genetic assessment of apple germplasm in Bosnia and Herzegovina using microsatellite and morphologic markers. Sci Hort 126(2):164–171
Godwin ID, Aitken EA, Smith LW (1997) Application of inter simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18(9):1524–1528
Goulão L, Oliveira CM (2001) Molecular characterisation of cultivars of apple (Malus domestica Borkh.) Using microsatellite (SSR and ISSR) markers. Euphytica 122:81–89. https://doi.org/10.1023/A:1012691814643
Hokanson SC, Lamboy WF, Szewc-McFadden AK, McFerson JR (2001) Microsatellite (SSR) variation in a collection of Malus (apple) species and hybrids. Euphytica 118:281–294. https://doi.org/10.1023/A:1017591202215
Juniper BE, Robinson J, Harris SA, Watkins R (1999) The origin of the apple. Acta Hort 484:27–34
Kabiri G, Bouda S, Elhansali M, Haddioui A (2019) Genetic diversity and structure of walnut (Juglans regia L.) genotypes from Middle and High Atlas Mountains of Morocco as investigated by Inter-Simple sequence repeat (ISSR) markers. Aust J Crop Sci 13(2):1983–1991
Khachtib Y, Bouda S, Ait Bella Y, Haddioui A (2020) Pomological evaluation of apple (Malus x domestica Borkh) germplasm in Morocco. Phytomorphology 70:1–9
Lassois L, Denancé C, Ravon E, Guyader A, Guisnel R, Hibrand-Saint-Oyant L, Poncet C, Lasserre-Zuber P, Feugey L, Durel CE (2016) Genetic diversity, population structure, parentage analysis, and construction of core collections in the french apple germplasm based on SSR markers. Plant Mol Biology Report 34(4):827–844. https://doi.org/10.1007/s11105-015-0966-7
Liang W, Dondini L, De Franceschi P, Paris R, Sansavini S, Tartarini S (2015) Genetic diversity, population structure and construction of a core collection of apple cultivars from italian germplasm. Plant Mol Biology Report 33(3):458–473. https://doi.org/10.1007/s11105-014-0754-9
Mariano LC, Zchonski FL, Da-Silva CM, Da-Silva PR (2019) Genetic variability in a brazilian apple germplasm collection with low chilling requirements. PeerJ 6:e6265. https://doi.org/10.7717/peerj.6265
Pathak H, Dhawan V (2012) ISSR assay for ascertaining genetic fidelity of micropropagated plants of apple rootstock Merton 793. Vitro Cell Dev Biology-Plant 48:137–143. https://doi.org/10.1007/s11627-011-9385-0
Pereira-Lorenzo S, Ramos-Cabrer AM, Ferreira V, Díaz-Hernández MB, Carnide V, Pinto-Carnide O, Rodrigues R, Velázquez-Barrera ME, Rios-Mesa D, Ascasíbar-Errasti J, Castro I (2018) Genetic diversity and core collection of Malus × domestica in northwestern Spain, Portugal and the Canary Islands by SSRs. Sci Hort 240:49–56. https://doi.org/10.1016/j.scienta.2018.05.053
Pereira-Lorenzo S, Ramos-Cabrer AM, Gonzalez-Diaz AJ, Diaz-Hernandez MB (2008) Genetic assessment of local apple cultivars from La Palma, Spain, using simple sequence repeats (SSRs). Sci Hort 117(2):160–166
Ping H, Li L, Li H, Wang H, Yang J, Wang Y (2011) Genetic analysis of wild apple resourcesin Shandong province based on inter-simple sequence repeats (ISSR) and sequencespecific amplification polymorphism (S-SAP) markers. Afr J Biotechnol 10:95019508. https://doi.org/10.5897/AJB11.1111
Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR fngerprinting of potato cultivars. Theor Appl Genet 98(1):107–112
Pritchard JK, Wen W, Falush D (2010) Documentation for STRUCTURE software: Version 2. University of Chicago, Chicago, IL
Roldàn-Ruiz I, Dendauw J, Van Bockstaele E, Depicker A, De Loose M (2000) AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp) molecular breeding 6. 125–134. https://doi.org/10.1023/A:1009680614564
Smolik M, Krzysztoszek O (2010) Evaluation of genetic variability in choosen apple (Malus × domestica Borkh.) Cultivars by ISSR-PCR analysis. Russian J Genet 46:819–827. https://doi.org/10.1134/S1022795410070069
Smolik M, Rzepka-Plevneš D, Stankiewicz I, Chelpiński P, Kowalczys K (2004) Analysis of genetic similarity of apple tree cultivars. Folia Horticulturae 16:87–94
Uzun A, Ozongun S, Gulsen O, Yılmaz KU, Kaymak S, Ercisli S (2016) Determination of genetic relatedness among turkish apple germplasm based on ISSR markers. J Appl Bot Food Qual 89:82–88. https://doi.org/10.5073/JABFQ.2016.089.010
Yeh FC, Yang RC, Boyle T (1999) POPGENE version 1.32: Microsoft Windows–based freeware for population genetic analysis, quick user guide. Center for International Forestry Research, University of Alberta, Edmonton, Alberta, Canada, pp 1–29
Zhang C, Chen X, He T, Liu X, Feng T, Yuan Z (2007) Genetic structure of Malus sieversii population from Xinjiang, China, revealed by SSR markers. J Genet Genomics 34(10):947–955