Vegetative propagation of Tetrapleura tetraptera Taub. from stem cuttings


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email:
Doi: 10.1007/s42535-022-00373-8
First Page: 978
Last Page: 984
Views: 106

Keywords: Tetrapleura tetraptera , Vegetative propagation, Cutting positions, Growth regulators, Seedling source


Tetrapleura tetraptera Taub. is a highly valued multipurpose tree species among households in Tropical Africa. The fruits are traded at premium prices, limiting their production through seed germination. Hence, clonal propagation of superior planting stocks would be beneficial. This study investigated different concentrations of growth regulators, cutting positions and stock plant source on growth of two-node stem cuttings. One thousand four hundred and forty cuttings were obtained and treatments were laid in split-split plot design. Data on percentage survival and rooting, number of roots, number of leaves, root length and shoot length were subjected to analysis of variance. Growth regulators did not have a significant effect on the analysed variables except root length. Percentage of rooted cuttings (65.9%), cutting’s survival (56.3%), number of roots (2.2), leaves (2.5), root length (2.8 cm) and shoot length (4.7 cm) were significantly higher in basal cuttings than the medial and apical. Highest cuttings survival (38.2%), rooting percentage (38.8%), number of roots (1.5), number of leaves (1.5), root length (1.6 cm) and shoot length (2.6 cm) were obtained in Mamu source. Tetrapleura tetraptera is amenable to vegetative propagation where stock plant source variation influenced rooting success while basal cuttings gave the best performance.

                Tetrapleura tetraptera
              , Vegetative propagation, Cutting positions, Growth regulators, Seedling source

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Achi OK (2006) Composition and antibacterial activities of Tetrapleura tetraptera Taub. pod extracts. Res J Microbiol 1(5):416–422

Adekola OF, Akpan IG (2012) Effects of growth hormones on sprouting and rooting of Jatropha curcas stem cuttings. J Appl Sci Environ Mgt 16(1):168

Adelaja BA, Fasidi IO (2012) Survey and collection of indigenous spice germplasm for conservation and genetic improvement in Nigeria. PGR Newsl (FAO Bioversity) 153:67–71

Akinyele AO (2010) Effects of growth hormones, rooting media and leaf size on juvenile stem cuttings of Buchholzia coriacea Engler. Ann for Res 53(2):127–133

Aladesanmi JA (2007) Tetrapleura tetraptera: Molluscicidal activity and chemical constituents. Afr J Tradit Compl Altern Med 4(1):23–36

Beemnet MK, Solomon AM (2012) Effect of cutting position and rooting hormone on propagation ability of Stevia rebaudiana (Bertoni). Afr J Plant Sci Biotechnol 6(1):5–8

Ekwenye UN, Okorie CF (2010) Antibacterial activity of Tetrapleura tetraptera Taub pod extracts. Int J Pharma Bio Sci 1(4):734–741

Essien EU, Izunwane BC, Aremu CY, Eka OU (1994) Significance for humans of the nutrient contents of the dry fruit of Tetrapleura tetraptera. Plant Food Human Nutr 45(1):47–51

Essien EU, Izunwane BC, Aremu CY, Eka OU (2009) Investigation into the toxic constituents of the dry fruit of Tetrapleura tetraptera. Int J Food Sci Nutr 44(1):55–58

Gbadamosi AE, Oni O (2005) Macropropagation of an endangered medicinal plant Enantia chlorantha oliv. J Arboricult 31(2):78–82

Gbadamosi AE (2014) Vegetative propagation of medicinal plant Picralima nitida (Stapf). Int J Pure Appl Sci Technol 23(1):13–27

Hartmann HT, Kester DE, Davies FT, Geneve RL (2002) Plant propagation: Principles and practices (7th ed. 849 pp) Prentice Hall Inc, New Jersey (Upper Saddle river).

Hossain MA, Islam MA, Hossain MM (2004) Rooting ability of cuttings of Swietenia macrophylla King and Chukrasia velutina Wight et Arn as influenced by exogenous hormone. Int J Agric Biol 6(3):150–154

Ibiang YB, Ita EE, Ekanem BE, Edu NE (2012) Effect of different pretreatment protocols on seed germination of Tetrapleura tetraptera (Schum and Thonn). IOSR-JESTFT 2(3):25–29

Kebede M, Hulten H, Balcha G (2013) Vegetative propagation of juvenile leafy stem cuttings of P africana (Hook. F.) Kalkm and Syzygium guineense (Willd.) DC. Int J Bot 9(1):30–36

Kesari V, Das A, Rangan L (2010) Effect of genotype and auxin treatments on rooting response in stem cuttings of CPTs Pongamia pinnata, a potential biodiesel legume crop. Curr Sci 98:9

Kuate D, Kengne AP, Biapa CP, Azantsa BG, Muda W (2015) Tetrapleura tetraptera spice attenuates high carbohydrate, high-fat diet-induced obese and type 2 diabetes rats with metabolic syndrome features. Lipids Health Dis 14(50):1–13

Leakey RRB (2014) Plant cloning: Macropropagation. In: Neal Van Alfen (eds.) Encyclopaedia of Agric and Food Systems San Diego Elsevier 4: 349–359.

Leakey RRB, Mese´n JF, Tchoundjeu Z, Longman KA, Dick JM, Newton A, Matin A, Grace J, Munro RC, Muthoka PN, (1990) Low technology techniques for the vegetative propagation of tropical trees. Commonw Rev 69(3):247–257

Ofori D, Newton AC, Leakey RRB, Grace J (1996) Vegetative propagation of Milicia excelsa by leafy stem cuttings: effects of auxin concentration, leaf area and rooting medium. Forest Ecol Mgt 84:39–48

Ogbimi ER, Sakpere AM, Akinropo SM (2020) Vegetative propagation of Afzelia Africana Sm.Ex Pers.: a multipurpose and threatened tree. Int J Biol Chem Sci 14(1):204–212

Ojewole JAO (2005) Analgesic and anticonvulsant properties of Tetrapleura tetraptera Taub (Fabaceae) fruit aqueous extract in mice. Phytotherapy Res 19(12):1023–1029

Ojewole JAO, Adewunmi CO (2004) Anti-inflammatory and hypoglycaemic effects of Tetrapleura tetraptera Taub. fruit aqueous extract in rats. J Ethnopharmacol 95 (2–3):177–182.

Okereke CN, Nnabude PC, Mbaekwe EI, Ekwealor KU, Uhuo CA, Nwanchor KC (2014) The use of ecological method in vegetative studies of plant species and abundance south-eastern Nigeria. Afr J Plant Sci 8(9):441–449

Okokon JE, Udokpoh AE, Antia BS (2007) Antimalaria activity of ethanolic extract of Tetrapleura tetraptera fruit. J Ethnopharmacol 111(3):537–540

Okunlola AI (2016) Effects of rooting hormones on the propagation of bougainvillea from cuttings. Int J Res Agric for 3(1):57–62

Okwu DE (2003) The potentials of Ocimum gratissimum, Pergularia extensa and Tetrapleura tetraptera fruit as spice and flavouring agents. Nig Agric J 35:143–148

Opabode JT, Akinyemiju OA, Ayeni OO (2011) Plant regeneration via somatic embryogenesis from immature leaves in Tetrapleura tetraptera (Schum. and Thonn.) Taub. Arch Biol Sci Belgrade 63 (4):1135–1145.

Saifuddin M, Osman N, Rahman MM (2013) Influence of different cutting positions and rooting hormones on root initiation and root-soil matrix of two tree species. Int J Agric Biol 15:427–434

Shekhawat MS, Manokari M (2016) Impact of auxins on vegetative propagation through stem cuttings of Couroupita guianensis Aubl. A conservation approach. Scientifica 7.

Shiembo PN, Newton AC, Leakey RRB, Grace J (1996) Vegetative propagation of Irvingia gabonensis, a West African fruit tree. Forest Ecol Mgt 87:185–192

Tate HT, Page T (2018) Cutting propagation of Santalum austrocaledoniicum: the effect of genotype, cutting source, cutting size, propagation medium, IBA and irradiance. New Forests.

Tchoundjeu Z, Leakey RRB (2000) Vegetative propagation of Khaya ivorensis (African mahogany): Effects of stockplant flushing cycle, auxin and leaf area on carbohydrate and nutrient dynamics of cuttings. J Trop for Sci 12(1):77–91

Tchoundjeu Z, Leakey RRB (2001) Vegetative propagation of Lovoa trichilioides: effects of provenance, substrate, auxins and leaf area. J Trop for Sci 13(1):116–129

Tsobeng A, Asaah E, Makueti J, Tchoundjeu Z, Van-Damme P (2013) Propagation of Pentaclethra macrophylla Benth (Fabaceae) through seed and rooting of leafy stem cuttings. Int J Agron Agric Res 3(12):10–20

Leakey RRB, Akinnifesi FK (2008) Towards a domestication strategy for indigenous fruit trees in the tropics. In: Akinnifesi FK, Leakey RRB, Ajayi OC, Sileshi G, Tchoundjeu Z, Matakala P, Kwesiga FR (eds) Indigenous fruit trees in the tropics: domestication, utilization and commercialization. CABI, UK, pp 28–49



Author Information

Olaniyi Aishat Adeola
Multipurpose Tree Improvement and Multiplication Unit, Forestry Research Institute of Nigeria, Ibadan, Nigeria
Adegeye Adebola Omodele
Department of Forest Production and Products, Faculty of Renewable Natural Resources, University of Ibadan, Ibadan, Nigeria