Vegetos
(An International Journal of Plant Research & Biotechnology)
α-Glucosidase inhibitors from an endophytic fungus Edenia gomezpompae from Costus speciosus
*Article not assigned to an issue yet
Nilmini B. M. S., Samarakoon Kavindya, Yakandawala Deepthi, Kumar Savitri, Adikaram Nimal, Jayasinghe Lalith, Araya Hiroshi, Fujimoto Yoshinori
Research Articles | Published: 23 July, 2025
First Page: 0
Last Page: 0
Views: 3
Keywords: n Edenia gomezpompaen , n Costus speciosusn , Endophyte, Leptosphaeridione, Preussomerin EG2n
Abstract
Costus speciosus is a plant used in traditional medicine. It is widely used as a treatment for diabetes in Sri Lanka, India and neighboring countries. An endophytic fungus, Edenia gomezpompae, isolated from the leaves of Costus speciosus, was cultured in potato dextrose broth media. Chromatographic separation of the EtOAc extract of the broth and mycelium led to the isolation of rare compounds leptosphaeridione (1) and preussomerin EG2 (2). This is the first report of the isolation of the endophytic fungus E. gomezpompae from C. speciosus. Both compounds were screened for their enzyme inhibitory activity toward α-glucosidase, α-amylase, lipase and acetylcholinesterase. Compounds 1 and 2 exhibited significant α-glucosidase inhibitory activity with the IC50 values of 2.39 μg/mL and 27.9 μg/mL, respectively. This study contributes to our knowledge on the diversity and chemical potential of fungal endophytes associated with medicinal plants.
References
Ahonsi MO, Maurhofer M, Boss D, Défago G (2005) Relationship between aggressiveness of Stagonospora sp. isolates on field and hedge bindweeds, and in vitro production of fungal metabolites cercosporin, elsinochrome A and leptosphaerodione. Eur J Plant Pathol 111(3):203–215
Alakolanga AGAW, Kumar NS, Jayasinghe L, Fujimoto Y (2015) Antioxidant property and α-glucosidase, α-amylase and lipase inhibiting activities of Flacourtia inermis fruits: characterization of malic acid as an inhibitor of the enzymes. J Food Sci Technol 52(12):8383–8388
Bandara HMSKH, Kumar NS, Jayasinghe L, Masubuti H, Fujimoto Y (2015) A 3-vinyl cephem derivative, useful intermediate in the synthesis of cephem antibiotics, from Aspergillus awamori associated with banana fruit. Nat Prod Commun 10(10):1663–1666
Berg A, Reiber K, Dorfelt H, Walther G, Schlegel B, Gräfe U (2000) Laccaridiones A and B, new protease inhibitors from Laccaria amethystea. J Antibiotics 53(11):1313–1316
Dissanayake D, Kumar NS, Adikaram NKB, Jayasinghe L, Araya H, Fujimoto Y (2020) Production of small molecules by an endophytic fungus, Neofusicoccum parvum from the fruits of Elaeocarpus serratus. Asian J Tradit Med 15(2):20–27
Fernando WIT, Attanayake AMKC, Perera HKI, Sivakanesan R, Jayasinghe L, Araya H, Fujimoto Y (2019) Isolation, identification and characterization of pancreatic lipase inhibitors from Trigonella foenum-graecum seeds. S Afr J Bot 121:418–421
Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 2(2):113–118
Guerriero A, D’Ambrosio M, Cuomo V, Pietra F (1991) A novel degraded polyketidic lactone, leptosphaerolide, and its likely diketone precursor, leptosphaerodione. Isolation from cultures of the marine ascomycete Leptosphaeria oraemaris (Linder). Helv Chim Acta 74(7):1445–1450
Kehelpannala CL, Kumar NS, Jayasinghe L, Araya H, Fujimoto Y (2018) Naphthoquinone metabolites produced by Monacrosporium ambrosium, the ectosymbiotic fungus of tea shot-hole borer, Euwallacea fornicatus, in stems of tea. Camellia Sinensis J Chem Ecol 44(1):95–101
Macías-Rubalcava ML, Hernández-Bautista BE, Jiménez-Estrada M, González MC, Glenn AE, Hanlin RT, Hernández-Ortega S, Saucedo-García A, Muria-González JM, Anaya AL (2008) Naphthoquinone spiroketal with allelochemical activity from the newly discovered endophytic fungus Edenia gomezpompae. Phytochemistry 69(5):1185–1196
Macías-Rubalcava M, Ruiz-Velesco Sobrino ME, Meléndez-González C, Hernandez-Ortega S (2014) Naphthquinone spiroketals and organic extracts from the endophytic fungus Edenia gomezpompae as potentiall herbicides. J Agric Food Chem 62(16):3553–3562
Munasinghe MVK, Kumar NS, Jayasinghe L, Fujimoto Y (2017) Indole-3-acetic acid production by Colletotrichum siamense, An endophytic fungus from Piper nigrum leaves. J Biol Act Prod Nat 7(6):475–479
Munasinghe MVK, Kumar NS, Adikaram NKB, Jayasinghe L, Araya H, Fujimoto Y (2021) Secondary metabolites from an endophytic fungus Aspergillus fumigatus from Solanum insanum L. Asian J Tradit Med 16(2):53–59
Nicolet B. 1999. Isolement et identification de métabolites secondaires de deux souches de champignon Stagonospora sp., agent pathogène du liseron des haies (Calystegia sepium) et du liseron des champs (Convolvulus arvensis). Travail de thèse, Faculté des Sciences, Université de Neuchâtel.
Padmathilake KGE, Bandara HMSKH, Qader MM, Kumar NS, Jayasinghe L, Masubuti H, Fujimoto Y (2017) Talarofuranone, a new talaroconvolutin analog from the endophytic fungus Talaromyces purpurogenus from Pouteria campechiana seeds. Nat Prod Commun 12(4):489–490
Piyasena KGNP, Wickramarachchi WART, Kumar NS, Jayasinghe L, Fujimoto Y (2015) Two phytotoxic azaphilone derivatives from Chaetomium globosum, a fungal endophyte isolated from Amaranthus viridis leaves. Mycology 6(3–4):158–160
Poch GK, Gloer JB (1989) Obionin A: A new polyketide metabolite from the marine fungus Leptosphaeria obiones. Tetrahedron Lett 30(27):3483–3486
Qader MM, Kumar NS, Jayasinghe L, Fujimoto Y (2016) Production of antitumor antibiotic GKK1032B by Penicillium citrinum, an endophytic fungus isolated from Garcinia mangostana fruits. Med Aromatic Plant 5(1):225
Qader MM, Kumar NS, Jayasinghe L, ArayaFujimoto HY (2017) Bioactive sesquiterpenes from an endophytic fungus Bipolaris sorokiniana isolated from a popular medicinal plant Costus speciosus. Mycology 8(1):17–20
Qader MM, Kumar NS, Jayasinghe L, Fujimoto Y (2018) Shikimic acid production by Fusarium decemcellulare, an endophytic fungus isolated from Flacourtia inermis fruits. J Biol Active Prod Nature 8(1):43–50
Rathnayake GRN, Kumar NS, Jayasinghe L, Araya H, Fujimoto Y (2018) Chemical investigation of metabolites produced by an endophytic fungi Phialemonium curvatum from the leaves of Passiflora edulis. Nat Prod Res 32(20):2483–2486
Rathnayake GRN, Kumar NS, Jayasinghe L, Araya H, Fujimoto Y (2019) Secondary metabolites produced by an endophytic fungus Pestalotiopsis microspora. Natural Products and Bioprospecting 9(6):411–417
Sathya S, Amarasinghe NR, Jayasinghe L, Araya H, Fujimoto Y (2020) Enzyme inhibitors from the aril of Myristica fragrans. South Afr J Botany 130(1):172–176
Siriwardane AMDA, Kumar NS, Jayasinghe L, Fujimoto Y (2015) Chemical investigation of metabolites produced by an endophytic Aspergillus sp. isolated from Limonia acidissima. Nat Prod Res 29(14):1384–1387
Sritharan T, Kumar NS, Jayasinghe L, Araya H, Fujimoto Y (2019) Isocoumarins and dihydroisocoumarins from the endophytic fungus Biscogniauxia capnodes isolated from the fruits of Averrhoa carambola. Nat Prod Commun 14(5):1934578X19851969
Strobel G, Daisy B, Castillo U, Harper J (2004) Natural products from endophytic microorganisms. J Nat Prod 67(2):257–268
Tan Y, Guo Z, Zhu M, Shi J, Li W, Jiao R, Tan R, Ge H (2020) Anti-inflammatory spirobisnaphthalene natural products from a plant-derived endophytic fungus Edenia gomezpompae. Chin Chem Lett 31(6):1406–1409
Thanabalasingam D, Kumar NS, Jayasinghe L, Fujimoto Y (2015) Endophytic fungus Nigrospora oryzae from a medicinal plant Coccinia grandis, a high yielding new source of phenazine-1-carboxamide. Nat Prod Commun 10(10):1659–1660
Thanabalasingam D, Dissanayake D, Samarakoon K, Kumar NS, Adikaram N, Jayasinghe L, Araya H, Fujimoto Y (2024) Oryzanigral: a new polyketide from an endophytic fungus Nigrospora oryzae isolated from Coccinia grandis. Nat Prod Res 38(15):2703–2710
White TJ, Bruns T, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, pp 315–322
Author Information
National Institute of Fundamental Studies, Kandy, Sri Lanka