Assessment of sex specific relationship among nine Dioscorea species found in Tripura by using morphometric and micro morphometric markers

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-019-00033-4
First Page: 254
Last Page: 268
Views: 1757


Keywords: Dioscorea , Morphometric and micro-morphometric traits, Principal component analysis, Cluster analysis, Conservation


Abstract


Present study deals the assessment of inter and intra specific relationship among nine Dioscorea species by using morphological and micro-morphological markers. To fabricate the relationship among the Dioscorea species 37 micro-morphometric traits were adopted. Wild Dioscorea tuber is a potential substitute food supplement offering the seasonal food security to the indigenous forest dwellers of Tripura. In nature, Dioscorea species exhibit high morphological variation. In spite of enormous information available regarding the interspecific relationship in dioecious Dioscorea species meager information are existing with respect to sexes. PCA analysis revealed that two vital components account for 58.21% of total variation among the traits. PCA I was strongly associated (r > 0.5) with fourteen morphological traits. The first seven PCs have Eigen value greater than 1 together explain 95.17% of the total variance. The results of cluster analysis and dendrogram developed initially produce four main groups at level 7.31. The results obtained may contribute to effective conservation and improvement practices of Dioscorea with good agronomical traits which may be attributable with the utilization of Dioscorea genetic resources.



                Dioscorea
              , Morphometric and micro-morphometric traits, Principal component analysis, Cluster analysis, Conservation


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References


  1. Aina OD, Atumeyi S (2011) Foliar epidermal anatomy of four species of Dioscorea. Adv Appl Sci Res 2(4):21–24

  2. Asha KI (2004) Characterization and micro-propagation of selected species of Dioscorea in Southern Western Ghats. Ph.D. thesis, University of Kerala, Kerala, India

  3. Asiedu R, Ng SYC, Bai KV, Ekanayake IJ, Wanyera NMW (1998) Genetic Improvement. In: Orkwor GC, Asiedu R, Ekanayake IJ (eds) Food yams: advances in research. IITA and NRCRI, Ibadan, pp 63–104

  4. Ayensu ES, Coursey DG (1972) Guinea yams: the botany, ethnobotany, use and possible future of yams in West. Africa Econ Bot 26:301–318

  5. Bhandari MR, Kasai T, Kawabata J (2003) Nutritional evaluation of wild yam (Dioscorea spp.) tubers of Nepal. Food Chem 82(4):619–623

  6. Burkill (1960) Organography and evolution of Dioscoreaceae, the family of yams. Bot J Linean Soc 56(367):319–412

  7. Caddick LR, Rudall PJ, Wilkin P, Hedderson TAJ, Chase MW (2002) Phylogenetics of Dioscoreales based on combined analyses of morphological and molecular data. J Linn Soc (Bot.) 138(2):123–144

  8. Coursey DG (1967) Yams: an account of the nature, origins, cultivation and utilisation of the useful member of the Dioscoreaceae. Longmans, London

  9. Dansi A, Mignouna HD, Zoundjihekpon J, Sangare A, Asiedu R, Quin FM (1999) Morphological diversity, cultivar groups and possible descent in the cultivated yams (Dioscorea cayenensis/D. rotundata) complex in Benin Republic. Genet Resour Crop Evol 46(4):371–388

  10. Dickison WC (2000) Integrative Plant Anatomy, 1st edn. Academic Press, San Diego

  11. Edeoga HO, Okoli BE (2001) Midrib anatomy and systematics in Dioscorea L (Dioscoreaceae). J Econ Tax Bot 19:191–195

  12. Garg A (2010) Micro-morphological key of the leaf epidermal features of Berberis L. taxa. J Econ Tax Bot 34(2):415–418

  13. Goswami N (2015) Taxonomic study of the genus Dioscorea L. occurring in Assam, India. Ph.D. thesis, Gauhati University, Guwahati, Assam, India

  14. Hodeba D, Mathew M, Abang M, Asiedu R, Geeta R (2013) True Yams (Dioscorea): a biological and evolutionary Link between Eudicots and Grasses. Cold Spring Harb protoc 3:46–55

  15. International Plant Genetic Resources Institute (IPGRI/IITA) (1997) Descriptors for yam (Dioscorea spp.) IPGRI, Rome, Italy. International Plant Genetic Resources Institute (IPGRI)/International Institute of Tropical Agriculture (IITA), Ibadan, p 61

  16. Kumar G, Subba RMV, Manga V (2007) Assessment of morphological variation in Dioscorea L. using numerical analysis. Phytomorphology 57(1):95–102

  17. Kumar S, Das G, Shin HS, Patra JK (2017) Dioscorea spp. (a wild edible tuber): a study on its ethnopharmacological potential and traditional use by the local people of Similipal Biosphere Reserve, India. Front pharmacol 8(52):1–17

  18. Lavania S (1990) Trichome morphology in Indian Solanum. J Indian Bot Soc 69:143–148

  19. Manly BFJ (1994) Multivariate statistical methods a primer, 2nd edn. Chapman and Hall, London, p 214

  20. Metcalfe CR (1961) The anatomical approach to systematics: general introduction with special reference to recent work on monocotyledons. Recent Adv Bot 1:146–150

  21. Metcalfe CR, Chalk L (1950) Anatomy of the Dicotyledons, vol 2. Clarendon Press, Oxford, p 840

  22. Metcalfe CR, Chalk L (1957) Anatomy of the dicotyledons, 1st edn. Clarendon Press, Oxford, pp 502–535

  23. Norman PE, Tongoona P, Shanahan PE (2011) Diversity of the morphological traits of yam (Dioscorea spp.) genotypes from Sierra Leone. J appl biosci 45:3045–3058

  24. Padmini S, Rao SRS (1995) Structure, distribution and taxonomic importance of foliar stomata in some Indian Amaranthaceae. Bot J Linn Soc 118(2):149–161

  25. Paul C, Debnath A, Debnath B (2018) A documentation on Dioscorea spp. (a neglected wild tuber) with special reference to its domestication and seasonal food security to the indigenous forest dwellers in Tripura, India. Amb Sci 5(2):51–53

  26. Saikia B, Rawat JS, Tag H, Das AK (2011) An investigation on the taxonomy and ecology of the genus Dioscorea in Arunachal Pradesh, India. JFR Arts Sci 1:44–53

  27. Seikh N, Kumar Y, Misra AK, Pinokiyo A (2009) Status documentation of Dioscorea L (Dioscoreaceae) in Meghalaya: an approach towards food security. Pleione 3(1):74–82

  28. Shah GL, Kothari MJ (1975) Observations on stomata and hairs on vegetative and floral organs in the Tribe Trifolieae (Family Papilionaceae). Aust J Bot 23(1):111

  29. Sheikh N, Kumar Y (2017) Morphological characterization of Meghalayan Dioscorea spp. (yam), North East India. J Agr Sci Tech 19(2):487–497

  30. Sokal RR, Michener CD (1958) A statistical method for evaluating systematic relationships. Univ Kansas Sci Bull 38(22):1409–1438

  31. Strgulc-Krajsek S, Dermastia M, Jogan N (2006) Determination key for Central European Epilobium species based on trichome morphology. Bot Helv 116(2):169–178

  32. Tamiru M, Maass BL, Pawelzik E (2008) Characterizing diversity in composition and pasting properties of tuber flour in yam germplasm (Dioscorea spp.) from Southern Ethiopia. J Sci Food Agric 88(10):1675–1685

  33. Uphof JC (1962) Plant Hairs, encyclopedia of plant anatomy IV. Borntraeger, Berlin

  34. Yang ZR, Lin Q (2005) Comparative morphology of the leaf epidermis in Schisandra (Schisandraceae). Bot J Lin Soc 148(1):39–56


  35.  


Acknowledgements


We are highly indebted to the CSIR, New Delhi, for their financial support [Ref. No. 38(1371)/13/EMR-II].


Author Information


Paul Chiranjit
Plant Diversity and Forest Biotechnology Laboratory, Department of Forestry and Biodiversity, Tripura University, Suryamaninagar, India
Chiranjit.paul869@gmail.com
Debnath Bimal
Plant Diversity and Forest Biotechnology Laboratory, Department of Forestry and Biodiversity, Tripura University, Suryamaninagar, India
bimalbc@rediffmail.com