Calcium-alginate coated synthetic seed production, storage and assessment of genetic stability in Alpinia galanga (L.) Willd

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-021-00314-x
First Page: 527
Last Page: 533
Views: 1017


Keywords: Alpinia galanga , Synthetic seed, Calcium-alginate, RAPD


Abstract


An efficient encapsulation method was developed for artificial seed production in Alpinia galanga. Shoot buds obtained from in vitro proliferated plantlets on Murashige and Skoog (MS) medium containing 2.5 mg/l 6-benzyladenine (BA) was used for encapsulation. Calcium-alginate matrix composed of 3% sodium (Na) alginate and 100 mM calcium chloride (CaCl2.2H2O) was optimum for uniform, firm synseed preparation. Highest germination and plantlet conversion (87%) was obtained on MS medium containing 2.0 mg/l BA and 0.5 mg/l α-naphthalene acetic acid (NAA) under light/dark (16/8 h) photoperiod. Synseeds stored at 4 °C exhibited 85% and 23% germination after 30 days and 120 days respectively. In vitro grown plantlets were hardened successfully to field condition with 76% survival rate. 8 random amplified polymorphic DNA (RAPD) markers were used to check the genetic stability of regenerated plants. A monomorphic banding profile confirmed the genetic similarity of plants obtained from synseeds.



                Alpinia galanga
              , Synthetic seed, Calcium-alginate, RAPD


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Acknowledgements



Author Information


Shamsudheen Kizhakke Modongal
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, India
shamsukm81@gmail.com
Mehaboob Valiyaparambath Musfir
Department of Botany, PSMO College, Tirurangadi, India


Faizal Kunnampalli
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, India


Raja Palusamy
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, India


Thiagu Ganesan
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, India

Raju Chellappan Soundar
Department of Botany, Vivekananda College, Madurai, India

Shajahan Appakan
Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli, India