Efficacy of mutagenic treatment with gamma-rays, EMS and combinations in producing superior mutants in okra (Abelmoschus esculentus L.)

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00372-9
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Keywords: n Abelmoschus esculentus, EMS, Gamma rays, Mutagenic effectiveness, Mutagenic efficiency


Abstract


Okra or lady’s finger (Abelmoschus esculentus (L.) Moench), also known as bhindi in India, belongs to the family Malvaceae. Broadening the genetic base through induced mutations is a supplementary tool that can lead to the development of genetic variability. The present experiment was undertaken to generate a broad genetic variability by determining mutagenic effectiveness and efficiency of gamma rays, EMS and their combinations in 526 lines of okra. The research was conducted in two generations namely M1 and M2 during 2014–2016 kharif season (May–September) at Experimental Farm, Department of Vegetable Science, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur (Himachal Pradesh). The parent material, selfed seeds of P-8variety were irradiated with 65, 75 and 85 kR doses of gamma rays and 1.2, 1.4 and 1.6% concentrations of EMS. The seeds along with control were space planted for raising M1 generation. Each M1 plant was harvested separately and desirable M1 individual plant progeny rows were laid in augmented design for raising M2 generation. The effectiveness and efficiency of the mutagen used was assessed from the data on biological damage in M1 generation. In M1 generation, results showed a dose dependent retardation in biological parameters like seed germination and plant survival; 75kR and 1.4% was depicted as LD50 values for both gamma rays and EMS, respectively. In M2 generation, 1.2% EMS had highest effectiveness (0.0431) and efficiency (2.02). The mutants with short internodal length, more number of nodes per plant, dwarf plant height, branched and high yield were isolated in M2 generation.


n                     Abelmoschus esculentus, EMS, Gamma rays, Mutagenic effectiveness, Mutagenic efficiency


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Acknowledgements


We extend our sincere thanks to the Department of Vegetable Science and Floriculture for providing laboratory as well as field facilities to carry out the research work. The authors also thank BARC, Trombay for giving gamma rays irradiation treatment to okra seeds.


Author Information


Gupta Nivedita
Department of Vegetable Science and Floriculture, College of Agriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India
guptanavi38@gmail.com
Sood Sonia
Department of Vegetable Science and Floriculture, College of Agriculture, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, Palampur, India