Vegetos
(An International Journal of Plant Research & Biotechnology)
GC–MS and FTIR characterization, unveiling phenolic content, antioxidant and antibacterial properties of the parasitic plant Cistanche violacea
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Research Articles | Published: 26 November, 2025
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Keywords: n Cistanche violacean , GC–MS, FTIR, Antioxidant assay, Antibacterial activity
Abstract
This study provides a comprehensive investigation to identify the phylogenetic classification of Cistanche violacea using internal transcribed spacer sequencing. Bioactive phytocompounds were screened using gas chromatography-mass spectrometry (GC–MS) and FTIR spectroscopy. Additionally, the antioxidant (ABTS, DPPH assay, and the total antioxidant capacity (TAC)) and antibacterial (MIC method) properties of the crude extract and fractions prepared from four solvents with increasing polarity were explored. Results revealed that chloroform, ethyl acetate, and dichloromethane exhibited strong antioxidant activities toward DPPH (IC50 = 15.46 ± 0.15 µg/ml), ABTS (IC50 = 2.94 ± 0.34 µg/ml), and TAC (IC50 = 1.65 ± 0.004 µg/ml), respectively. GC–MS and FTIR analysis identified several functional groups and promising bioactive molecules in the DCM, ethyl acetate, and chloroform fractions. Notably, cyclodecane (in chloroform) and cyclopropanes (in ethyl acetate). Concerning antibacterial activity, most tested extracts were particularly effective against Listeria monocytogenes ATCC 19115 and had suppressed methicillin-resistant Staphylococcus aureus ATCC 43300 more effectively than gentamicin. These findings provide concrete evidence for the biological potential of C. violacea constituents.
References
Aldughayman M, Thorogood CJ, Al-Mayah AA, Hawkins JA (2024) An account of the genus Cistanche (Orobanchaceae) in Iraq and taxonomic considerations in the Middle East. PhytoKeys 238:281–294. https://doi.org/10.3897/phytokeys.238.116470
Alia F, Chouikh A, Djahra AB, Brahim AB, Sadok N, Tliba A (2021) Comparative study of some physicochemical and biological properties of effect host species variation on the relationship Saharan parasitic plant Cistanche violacea (Desf.) Beck. Notsci Biol 13(4):11054–11054. https://doi.org/10.15835/nsb13411054
Ataei N, Schneeweiss GM, García MA, Krug M, Lehnert M, Valizadeh J, Quandt D (2020) A multilocus phylogeny of the non-photosynthetic parasitic plant Cistanche (Orobanchaceae) refutes current taxonomy and identifies four major morphologically distinct clades. Mol Phylogenet Evol 151:106898. https://doi.org/10.1016/j.ympev.2020.106898
Attia IB, Zucca P, Marincola FC, Nieddu M, Piras A, Rosa A, Rescigno A, Chaieb M (2020) Evaluation of the antioxidant and cytotoxic activities on cancer cell line of extracts of parasitic plants harvested in Tunisia. Pol J Food Nutr Sci 70(3):253–263. https://doi.org/10.31883/pjfns/122040
Baliyan S, Mukherjee R, Priyadarshini A, Vibhuti A, Gupta A, Pandey RP, Chang C-M (2022) Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules 27(4):1326. https://doi.org/10.3390/molecules27041326
Benjamaa R, Elbouny H, Errati H, Moujanni A, Kaushik N, Gupta R, Ennibi O, Nasser B, Choi EH, Kaushik NK (2024) Comparative evaluation of antioxidant activity, total phenolic content, anti-inflammatory, and antibacterial potential of Euphorbia-derived functional products. Front Pharmacol 15:1345340. https://doi.org/10.3389/fphar.2024.1345340
Bougandoura A, D’Abrosca B, Ameddah S, Scognamiglio M, Mekkiou R, Fiorentino A, Benayache S, Benayache F (2016) Chemical constituents and in vitro anti-inflammatory activity of Cistanche violacea Desf. (Orobanchaceae) extract. Fitoterapia 109:248–253. https://doi.org/10.1016/j.fitote.2016.01.010
Bouzayani B, Koubaa I, Frikha D, Samet S, Ben Younes A, Chawech R, Maalej S, Allouche N, Mezghani Jarraya R (2022) Spectrometric analysis, phytoconstituents isolation and evaluation of in vitro antioxidant and antimicrobial activities of Tunisian Cistanche violacea (Desf). Chem Pap 76(5):3031–3050. https://doi.org/10.1007/s11696-022-02082-7
Cai RL, Yang MH, Shi Y, Chen J, Li YC, Qi Y (2010) Antifatigue activity of phenylethanoid-rich extract from Cistanche deserticola. Phytother Res 24(2):313–315. https://doi.org/10.1002/ptr.2927
Chen H, Jing FC, Li CL, Tu PF, Zheng QS, Wang ZH (2007) Echinacoside prevents the striatal extracellular levels of monoamine neurotransmitters from diminution in 6-hydroxydopamine lesion rats. J Ethnopharmacol 114(3):285–289. https://doi.org/10.1016/j.jep.2007.07.035
Chirane MS (2019) Antioxydant and antimicrobial activities of essential oil and ethanol extract of Santolina chamaecyparissus L. Agrobiologia 9(2):1660–1668
Cloeckaert A, Pletzer D, Khaiboullina S (2025) Editorial: Reviews in microbial pathogenesis. Front. Microbiol. 16. https://doi.org/10.3389/fmicb.2025.1568840.
Debouba M, Balti R, Hwiwi S, Zouari S (2012) Antioxidant capacity and total phenols richness of Cistanche violacea hosting Zygophyllum album. Int J Phytomed 4(3):399–402
Djemouaı N, Saad S, Baali F, Meguellati H, Benzetta H, Foughalia A (2024) The parasitic plant Cistanche violacea (Desf.) Beck from Ghardaïa (Algeria): a source of biologically active compounds. J Turkish Chem Soc Sect a: Chem 11(2):433–452. https://doi.org/10.18596/jotcsa.1291696
Elferjane MR, Jovanović AA, Milutinović V, Čutović N, Jovanović Krivokuća M, Marinković A (2023) From Aloe vera leaf waste to the extracts with biological potential: optimization of the extractions, physicochemical characterization, and biological activities. Plants 12(14):2744. https://doi.org/10.3390/plants12142744
El-hashash M, Abdel-Gawad MM, El-Sayed MM, Sabry WA, Abdel-Hameed E-SS, Abdel-Lateef EE-S (2010) Antioxidant properties of methanolic extracts of the leaves of seven Egyptian Cassia species. Acta Pharm 60(3):361–367. https://doi.org/10.2478/v10007-010-0030-y
Fahmy G, El-Tantawy H, Abd El-Ghani M (1996) Distribution, host range and biomass of two species of Cistanche and Orobanche cernua parasitizing the roots of some Egyptian xerophytes. J Arid Environ 34(3):263–276. https://doi.org/10.1006/jare.1996.0108
Guo Y, Song G, Sun M, Wang J, Wang Y (2020) Prevalence and therapies of antibiotic-resistance in staphylococcus aureus. Front Cell Infect Microbiol. https://doi.org/10.3389/fcimb.2020.00107
Hammiche V, Maiza K (2006) Traditional medicine in Central Sahara: pharmacopoeia of Tassili N’ajjer. J Ethnopharmacol 105(3):358–367. https://doi.org/10.1016/j.jep.2005.11.028
Hayat J, Akodad M, Moumen A, Baghour M, Skalli A, Ezrari S, Belmalha S (2020) Phytochemical screening, polyphenols, flavonoids and tannin content, antioxidant activities and FTIR characterization of Marrubium vulgare L. from 2 different localities of Northeast of Morocco. Heliyon. https://doi.org/10.1016/j.heliyon.2020.e05609
Jin M, Zhao K, Huang Q, Xu C, Shang P (2012) Isolation, structure and bioactivities of the polysaccharides from Angelica sinensis (Oliv.) Diels: a review. Carbohyd Polym 89(3):713–722. https://doi.org/10.1016/j.carbpol.2012.04.049
Kenubih A, Belay E, Lemma K (2021) Evaluation of the antimicrobial activity of leaf extracts of Acokanthera schimperi against various disease-causing bacteria. JEP 13:889–899. https://doi.org/10.2147/JEP.S322396
Kim D-O, Chun OK, Kim YJ, Moon H-Y, Lee CY (2003) Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem 51(22):6509–6515. https://doi.org/10.1021/jf0343074
Lu M-C (1998) Studies on the sedative effect of Cistanche deserticola. J Ethnopharmacol 59(3):161–165. https://doi.org/10.1016/S0378-8741(97)00108-6
Mattana CM, Satorres SE, Sosa A, Fusco M, Alcará LE (2010) Antibacterial activity of extracts of acacia aroma against methicillin-resistant and methicillin-sensitive Staphylococcus. Braz J Microbiol 41:581–587. https://doi.org/10.1590/S1517-83822010000300007
Migabo H, Journal J (2023) Evaluation of phytochemical profile and antimicrobial activity of Tragia brevipes extracts against pathogenic bacteria. J Adv Biotechnol Exp Ther. https://doi.org/10.5455/JABET.2023.D113
Molnar M, Jakovljević Kovač M, Pavić V (2024) A comprehensive analysis of diversity, structure, biosynthesis and extraction of biologically active tannins from various plant-based materials using deep eutectic solvents. Molecules 29(11):2615. https://doi.org/10.3390/molecules29112615
Musa A, El-Massry KF, El-Ghorab AH, Farouk A, Ali HM, Abdelgawad MA, Naguib IA, Mostafa EM (2021) Volatile constituents of Cistanche tubulosa and their antioxidant and antimicrobial potentials. Recnatprod 15:301–312. https://doi.org/10.25135/rnp.220.20.11.1859
Nandiyanto ABD, Oktiani R, Ragadhita R (2019) How to read and interpret FTIR spectroscope of organic material. Indones J Sci Technol 4(1):97–118. https://doi.org/10.17509/ijost.v4i1.15806
Noreen H, Semmar N, Farman M, McCullagh JS (2017) Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus. Asian Pac J Trop Med 10(8):792–801. https://doi.org/10.1016/j.apjtm.2017.07.024
Piwowarczyk R, Ochmian I, Lachowicz S, Kapusta I, Sotek Z, Błaszak M (2020) Phytochemical parasite-host relations and interactions: a Cistanche armena case study. Sci Total Environ 716:137071. https://doi.org/10.1016/j.scitotenv.2020.137071
Priyanthi C, Sivakanesan R (2021) The total antioxidant capacity and the total phenolic content of rice using water as a solvent. Int J Food Sci 2021(1):5268584. https://doi.org/10.1155/2021/5268584
Rouis-Soussi LS, El Ayeb-Zakhama A, Mahjoub A, Flamini G, Jannet HB, Harzallah-Skhiri F (2014) Chemical composition and antibacterial activity of essential oils from the Tunisian Allium nigrum L. EXCLI J 13:526
Šamec D, Karalija E, Šola I, Vujčić Bok V, Salopek-Sondi B (2021) The role of polyphenols in abiotic stress response: the influence of molecular structure. Plants 10(1):118. https://doi.org/10.3390/plants10010118
Sharma P, Kalita MC, Thakur D (2016) Broad spectrum antimicrobial activity of forest-derived soil actinomycete, Nocardia sp. PB-52. Front Microbiol 7:347. https://doi.org/10.3389/fmicb.2016.00347
Simorangkir M, Hutabarat W, Nainggolan B, Silaban S (2019) Antioxidant and antibacterial activities of nonpolar to polar solvent extracts of Sarang Banua (Clerodenrum fragrans Vent Willd) leaves. RJC 12:959–965. https://doi.org/10.31788/RJC.2019.1225095
Smith JD, Woldemariam MG, Mescher MC, Jander G, Moraes CMD (2016) Glucosinolates from host plants influence growth of the parasitic plant Cuscuta gronovii and its susceptibility to aphid feeding. Plant Physiol 172:181. https://doi.org/10.1104/pp.16.00613
Sreepian A, Popruk S, Nutalai D, Phutthanu C, Sreepian PM (2022) Antibacterial activities and synergistic interaction of Citrus essential oils and Limonene with Gentamicin against clinically isolated Methicillin-Resistant Staphylococcus aureus. Sci World J 2022:8418287. https://doi.org/10.1155/2022/8418287
Sun CQ, O’Connor CJ, Roberton AM (2003) Antibacterial actions of fatty acids and monoglycerides against Helicobacter pylori. FEMS Immunol Med Microbiol 36(1–2):9–17. https://doi.org/10.1016/S0928-8244(03)00008-7
Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees evolution. Mol Biol 10(3):512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729. https://doi.org/10.1093/molbev/mst197
Tan LT-H, Ser H-L, Yin W-F, Chan K-G, Lee L-H, Goh B-H (2015) Investigation of antioxidative and anticancer potentials of Streptomyces sp. MUM256 isolated from Malaysia mangrove soil. Front Microbiol 6:1316. https://doi.org/10.3389/fmicb.2015.01316
Tew WY, Ying C, Wujun Z, Baocai L, Yoon TL, Yam MF, Jingying C (2022) Application of FT-IR spectroscopy and chemometric technique for the identification of three different parts of Camellia nitidissima and discrimination of its authenticated product. Front Pharmacol 13:931203. https://doi.org/10.3389/fphar.2022.931203
Timizar Z, Boutemak K, Hadj Ziane-Zafour A, Touzout N, Tahraoui H, Jaouadi B, Zamouche M, Kebir M, Zhang J, Amrane A (2024) Comprehensive analysis of phytochemical composition, antioxidant potential, and antibacterial activity of T. polium. Separations 11(4):90. https://doi.org/10.3390/separations11040090
Tourabi M, Metouekel A, Ghouizi AEL, Jeddi M, Nouioura G, Laaroussi H, Hosen ME, Benbrahim KF, Bourhia M, Salamatullah AM, Nafidi H-A, Wondmie GF, Lyoussi B, Derwich E (2023) Efficacy of various extracting solvents on phytochemical composition, and biological properties of Mentha longifolia L. leaf extracts. Sci Rep 13:18028. https://doi.org/10.1038/s41598-023-45030-5
Truong D-H, Nguyen DH, Ta NTA, Bui AV, Do TH, Nguyen HC (2019) Evaluation of the use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. J Food Qual 2019:8178294. https://doi.org/10.1155/2019/8178294
Vollmer W, Blanot D, de Pedro MA (2008) Peptidoglycan structure and architecture. FEMS Microbiol Rev 32:149–167. https://doi.org/10.1111/j.1574-6976.2007.00094.x
Waltrich KK, Hoscheid J, Prochnau IS (2015) ntimicrobial activity of crude extracts and fractions of Vernonia polyanthes Less (assa-peixe) flowers. A Rev Bras Plantas Med 17:909–914. https://doi.org/10.1590/1983-084X/14_110
Wang N, Ji S, Zhang H, Mei S, Qiao L, Jin X (2017) Herba Cistanches: anti-aging. Aging Dis 8(6):740. https://doi.org/10.14336/AD.2017.0720
Wasihun Y, Alekaw Habteweld H, Dires Ayenew K (2023) Antibacterial activity and phytochemical components of leaf extract of Calpurnia aurea. Sci Rep 13:9767. https://doi.org/10.1038/s41598-023-36837-3
Wu L, Xiang T, Chen C, Isah MB, Zhang X (2023) Studies on Cistanches herba: a bibliometric analysis. Plants 12(5):1098. https://doi.org/10.3390/plants12051098
Xue Z, Yang B (2016) Phenylethanoid glycosides: research advances in their phytochemistry, pharmacological activity and pharmacokinetics. Molecules 21(8):991. https://doi.org/10.3390/molecules21080991
Yamada P, Iijima R, Han J, Shigemori H, Yokota S, Isoda H (2010) Inhibitory effect of acteoside isolated from Cistanche tubulosa on chemical mediator release and inflammatory cytokine production by RBL-2H3 and KU812 cells. Planta Med 76(14):1512–1518. https://doi.org/10.1055/s-0030-1249775
Zara S, Petretto GL, Mannu A, Zara G, Budroni M, Mannazzu I, Multineddu C, Pintore G, Fancello F (2021) Antimicrobial activity and chemical characterization of a non-polar extract of saffron stamens in food matrix. Foods 10:703. https://doi.org/10.3390/foods10040703
Zothanpuia, Passari AK, Chandra P, Leo VV, Mishra VK, Kumar B, Singh BP (2017) Production of potent antimicrobial compounds from Streptomyces cyaneofuscatus associated with fresh water sediment. Front Microbiol 8:68. https://doi.org/10.3389/fmicb.2016.00347
Zou T-B, He T-P, Li H-B, Tang H-W, Xia E-Q (2016) The structure-activity relationship of the antioxidant peptides from natural proteins. Molecules 21(1):72. https://doi.org/10.3390/molecules21010072
Author Information
Laboratory of Sustainable Management of Natural Resources in Arid and Semi-Arid Areas, Faculty of Natural and Life Sciences, University of Naama, Naama, Algeria